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Found 1 Document (1.867 seconds)

BMRB: 5202

2ILX

Assignment of the 1H, 13C and 15N resonances of the catalytic domain of the rat 2',3'-cyclic nucleotide 3'-phosphodiesterase

Authors

Kozlov, G., Lee, J., Gravel, M., Ekiel, I., Braun, P.E., Gehrin, K.

Assembly

CNP catalytic domain

Entity

1. CNP catalytic domain (polymer, Thiol state: all free), 219 monomers, 24217.51 Da Detail

GSHMFLPLYF GWFLTKKSSE TLRKAGQVFL EELGNHKAFK KELRHFISGD EPKEKLDLVS YFGKRPPGVL HCTTKFCDYG KATGAEEYAQ QDVVRRSYGK AFKLSISALF VTPKTAGAQV VLNEQELQLW PSDLDKPSSS ESLPPGSRAH VTLGCAADVQ PVQTGLDLLE ILQQVKGGSQ GEEVGELPRG KLYSLGKGRW MLSLAKKMEV KAIFTGYYG

Formula weight

24217.51 Da

Source organism

Rattus norvegicus

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.3 %, Completeness: 67.6 %, Completeness (bb): 93.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	67.6 % (1746 of 2584)	68.6 % (925 of 1349)	61.7 % (624 of 1011)	87.9 % (197 of 224)
Backbone	93.4 % (1207 of 1292)	92.4 % (416 of 450)	93.7 % (594 of 634)	94.7 % (197 of 208)
Sidechain	48.6 % (723 of 1488)	56.6 % (509 of 899)	37.3 % (214 of 573)	0.0 % (0 of 16)
Aromatic	6.5 % (15 of 230)	9.6 % (11 of 115)	3.6 % (4 of 112)	0.0 % (0 of 3)
Methyl	59.2 % (135 of 228)	94.7 % (108 of 114)	23.7 % (27 of 114)

1. 2',3'-cyclic nucleotide 3'-phosphodiesterase

Show sample condition

Sample #1

Temperature 310 (±1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	2',3'-cyclic nucleotide 3'-phosphodiesterase	[U-13C; U-15N; U-90% 2H]		1.0 mM
2	NaCl			0.15 M

Sample #2

Temperature 310 (±1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	2',3'-cyclic nucleotide 3'-phosphodiesterase	[U-15N]		1.0 ~ 2.0 mM
4	NaCl			0.15 M

Sample #3

Temperature 310 (±1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	2',3'-cyclic nucleotide 3'-phosphodiesterase	[U-13C; U-15N]		1.0 mM
6	NaCl			0.15 M

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.07 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.07 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.08 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.0 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2ILX, Strand ID: A Detail

Release date

2002-05-06

Citation

Assignment of the 1H, 13C and 15N resonances of the catalytic domain of the rat 2',3'-cyclic nucleotide 3'-phosphodiesterase
Kozlov, G., Lee, J., Gravel, M., Ekiel, I., Braun, P.E., Gehrin, K.
J. Biomol. NMR (2002), 22, 99-100, PubMed 11885989 , DOI: , Abstract

Related entities 1. CNP catalytic domain, : 1 : 2 : 43 entities Detail

Interaction partners 1. CNP catalytic domain, : 2 interactors Detail

More details for 2 interactors identified by 2 citations

Experiments performed 7 experiments Detail

1 3D HNCA

Instrument

#1 Model Varian UnityPlus (800 MHz)

#2 Model Bruker DRX (500 MHz)

Sample #1

Temperature 310 (±1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	2',3'-cyclic nucleotide 3'-phosphodiesterase	[U-13C; U-15N; U-90% 2H]		1.0 mM
2	NaCl			0.15 M

Sample #2

Temperature 310 (±1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	2',3'-cyclic nucleotide 3'-phosphodiesterase	[U-15N]		1.0 ~ 2.0 mM
4	NaCl			0.15 M

Sample #3

Temperature 310 (±1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	2',3'-cyclic nucleotide 3'-phosphodiesterase	[U-13C; U-15N]		1.0 mM
6	NaCl			0.15 M

2 3D HNCACB

Instrument

#1 Model Varian UnityPlus (800 MHz)

#2 Model Bruker DRX (500 MHz)

Sample #1

Temperature 310 (±1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	2',3'-cyclic nucleotide 3'-phosphodiesterase	[U-13C; U-15N; U-90% 2H]		1.0 mM
2	NaCl			0.15 M

Sample #2

Temperature 310 (±1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	2',3'-cyclic nucleotide 3'-phosphodiesterase	[U-15N]		1.0 ~ 2.0 mM
4	NaCl			0.15 M

Sample #3

Temperature 310 (±1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	2',3'-cyclic nucleotide 3'-phosphodiesterase	[U-13C; U-15N]		1.0 mM
6	NaCl			0.15 M

3 3D CBCA(CO)NH

Instrument

#1 Model Varian UnityPlus (800 MHz)

#2 Model Bruker DRX (500 MHz)

Sample #1

Temperature 310 (±1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	2',3'-cyclic nucleotide 3'-phosphodiesterase	[U-13C; U-15N; U-90% 2H]		1.0 mM
2	NaCl			0.15 M

Sample #2

Temperature 310 (±1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	2',3'-cyclic nucleotide 3'-phosphodiesterase	[U-15N]		1.0 ~ 2.0 mM
4	NaCl			0.15 M

Sample #3

Temperature 310 (±1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	2',3'-cyclic nucleotide 3'-phosphodiesterase	[U-13C; U-15N]		1.0 mM
6	NaCl			0.15 M

4 3D HNCO

Instrument

#1 Model Varian UnityPlus (800 MHz)

#2 Model Bruker DRX (500 MHz)

Sample #1

Temperature 310 (±1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	2',3'-cyclic nucleotide 3'-phosphodiesterase	[U-13C; U-15N; U-90% 2H]		1.0 mM
2	NaCl			0.15 M

Sample #2

Temperature 310 (±1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	2',3'-cyclic nucleotide 3'-phosphodiesterase	[U-15N]		1.0 ~ 2.0 mM
4	NaCl			0.15 M

Sample #3

Temperature 310 (±1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	2',3'-cyclic nucleotide 3'-phosphodiesterase	[U-13C; U-15N]		1.0 mM
6	NaCl			0.15 M

5 3D 1H-13C-1H HCCH-TOCSY

Instrument

#1 Model Varian UnityPlus (800 MHz)

#2 Model Bruker DRX (500 MHz)

Sample #1

Temperature 310 (±1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	2',3'-cyclic nucleotide 3'-phosphodiesterase	[U-13C; U-15N; U-90% 2H]		1.0 mM
2	NaCl			0.15 M

Sample #2

Temperature 310 (±1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	2',3'-cyclic nucleotide 3'-phosphodiesterase	[U-15N]		1.0 ~ 2.0 mM
4	NaCl			0.15 M

Sample #3

Temperature 310 (±1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	2',3'-cyclic nucleotide 3'-phosphodiesterase	[U-13C; U-15N]		1.0 mM
6	NaCl			0.15 M

6 3D 1H-13C-1H NOESY

Instrument

#1 Model Varian UnityPlus (800 MHz)

#2 Model Bruker DRX (500 MHz)

Sample #1

Temperature 310 (±1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	2',3'-cyclic nucleotide 3'-phosphodiesterase	[U-13C; U-15N; U-90% 2H]		1.0 mM
2	NaCl			0.15 M

Sample #2

Temperature 310 (±1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	2',3'-cyclic nucleotide 3'-phosphodiesterase	[U-15N]		1.0 ~ 2.0 mM
4	NaCl			0.15 M

Sample #3

Temperature 310 (±1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	2',3'-cyclic nucleotide 3'-phosphodiesterase	[U-13C; U-15N]		1.0 mM
6	NaCl			0.15 M

7 3D 1H-1H-15N NOESY

Instrument

#1 Model Varian UnityPlus (800 MHz)

#2 Model Bruker DRX (500 MHz)

Sample #1

Temperature 310 (±1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	2',3'-cyclic nucleotide 3'-phosphodiesterase	[U-13C; U-15N; U-90% 2H]		1.0 mM
2	NaCl			0.15 M

Sample #2

Temperature 310 (±1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	2',3'-cyclic nucleotide 3'-phosphodiesterase	[U-15N]		1.0 ~ 2.0 mM
4	NaCl			0.15 M

Sample #3

Temperature 310 (±1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	2',3'-cyclic nucleotide 3'-phosphodiesterase	[U-13C; U-15N]		1.0 mM
6	NaCl			0.15 M

nullKeywords CNP, phosphodiesterase, NMR resonance assignments

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Found 1 Document (1.867 seconds)

BMRB: 5202

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. CNP catalytic domain, : 1 : 2 : 43 entities Detail

Interaction partners 1. CNP catalytic domain, : 2 interactors Detail

Experiments performed 7 experiments Detail

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