BMRB: 5203

1H, 13C, 15N chemical shift assignments for cysteine-rich domain of KSR

Authors

Zhou, M., Horita, D.A., Waugh, D.S., Byrd, R.A., Morrison, D.K.

Assembly

cysteine-rich domain of KSR

Entity

1. Kinase suppressor of Ras (polymer, Thiol state: free and other bound), 49 monomers, 5570.589 Da Detail

GSVTHRFSTK SWLSQVCNVC QKSMIFGVKC KHCRLKCHNK CTKEAPACR

2. ZN (non-polymer), 65.409 × 2 Da

Total weight

5701.4067 Da

Max. entity weight

5570.589 Da

Entity Connection

disulfide 8 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:HIS5:ND1	2:ZN1:ZN
2	disulfide	sing	1:CYS30:SG	2:ZN1:ZN
3	disulfide	sing	1:CYS33:SG	2:ZN1:ZN
4	disulfide	sing	1:CYS48:SG	2:ZN1:ZN
5	disulfide	sing	1:CYS17:SG	2:ZN1:ZN
6	disulfide	sing	1:CYS20:SG	2:ZN1:ZN
7	disulfide	sing	1:HIS38:ND1	2:ZN1:ZN
8	disulfide	sing	1:CYS41:SG	2:ZN1:ZN

Source organism

Mus musculus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 74.4 %, Completeness (bb): 79.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	74.4 % (429 of 577)	78.9 % (240 of 304)	66.4 % (146 of 220)	81.1 % (43 of 53)
Backbone	79.1 % (231 of 292)	89.9 % (89 of 99)	68.3 % (99 of 145)	89.6 % (43 of 48)
Sidechain	69.6 % (231 of 332)	69.3 % (142 of 205)	73.0 % (89 of 122)	0.0 % (0 of 5)
Aromatic	6.8 % (3 of 44)	13.6 % (3 of 22)	0.0 % (0 of 21)	0.0 % (0 of 1)
Methyl	97.4 % (37 of 38)	100.0 % (19 of 19)	94.7 % (18 of 19)

1. Kinase suppressor of Ras

GSVTHRFSTK SWLSQVCNVC QKSMIFGVKC KHCRLKCHNK CTKEAPACR

Show sample condition

Sample

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Kinase suppressor of Ras	[U-15N; U-13C]		1.0 mM
2	ZINC (II) ION			2.0 mM

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LACS Plot; CA

Referencing offset: -0.62 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CB

Referencing offset: -0.62 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; HA

Referencing offset: -0.1 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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Protein Blocks Logo

Calculated from 1 models in PDB: 1KBE, Strand ID: A Detail

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Release date

2002-05-06

Citation

Solution structure and functional analysis of the cysteine-rich C1 domain of kinase suppressor of Ras (KSR)
Zhou, M., Horita, D.A., Waugh, D.S., Byrd, R.A., Morrison, D.K.
J. Mol. Biol. (2002), 315, 435-446, PubMed 11786023 , DOI 10.1006/jmbi.2001.5263 , Abstract

Related entities 1. Kinase suppressor of Ras, : 1 : 1 : 2 : 173 entities Detail

Interaction partners 1. Kinase suppressor of Ras, : 15 interactors Detail

Experiments performed 7 experiments Detail