BMRB: 5210

NMR Solution Structure of sTva47, the Viral-Binding Domain of Tva

Authors

Tonelli, M., Peters, R.J., James, T.L., Agard, D.A.

Assembly

subgroup A Rous Sarcoma virus receptors PG800 and PG950

Entity

1. subgroup A Rous Sarcoma virus receptors PG800 and PG950 (polymer, Thiol state: all disulfide bound), 47 monomers, 5114.428 Da Detail

ISEFGSCPPG QFRCSEPPGA HGECYPQDWL CDGHPDCDDG RDEWGCG

Formula weight

5114.428 Da

Entity Connection

disulfide 3 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	0:CYS7:SG	1:CYS24:SG
2	disulfide	sing	0:CYS14:SG	1:CYS37:SG
3	disulfide	sing	0:CYS31:SG	1:CYS46:SG

Source organism

Callipepla

Exptl. method

NMR

Refine. method

simulated annealing with torsion angle dynamics (DYANA) followed by molecular dynamics (AMBER)

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 84.4 %, Completeness (bb): 81.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	84.4 % (416 of 493)	90.7 % (235 of 259)	73.5 % (139 of 189)	93.3 % (42 of 45)
Backbone	81.9 % (221 of 270)	96.9 % (93 of 96)	67.7 % (90 of 133)	92.7 % (38 of 41)
Sidechain	89.3 % (234 of 262)	87.1 % (142 of 163)	92.6 % (88 of 95)	100.0 % (4 of 4)
Aromatic	76.7 % (46 of 60)	76.7 % (23 of 30)	75.0 % (21 of 28)	100.0 % (2 of 2)
Methyl	100.0 % (10 of 10)	100.0 % (5 of 5)	100.0 % (5 of 5)

1. subgroup A Rous Sarcoma virus receptors

ISEFGSCPPG QFRCSEPPGA HGECYPQDWL CDGHPDCDDG RDEWGCG

Show sample condition

Sample

Pressure 1 atm, Temperature 313 K, pH 5.5

#	Name	Isotope labeling	Type	Concentration
1	subgroup A Rous Sarcoma virus receptors	[U-15N; U-13C]		2 mM
2	sodium acetate	[U-2H]		50 mM
3	calcium cloride			5 mM
4	D2O			10 %
5	H2O			90 %

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Calculated from 20 models in PDB: 1K7B, Strand ID: A Detail

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Release date

2002-02-04

Citation

The solution structure of the viral binding domain of Tva, the cellular receptor for subgroup A avian leukosis and sarcoma virus
Tonelli, M., Peters, R.J., James, T.L., Agard, D.A.
FEBS Lett. (2001), 509, 161-168, PubMed 11768384 , DOI: , Abstract

Related entities 1. subgroup A Rous Sarcoma virus receptors PG800 and PG950, : 1 : 2 : 750 entities Detail

Experiments performed 2 experiments Detail