BMRBj - BMREntryMaster

Found 1 Document (1.12 seconds)

BMRB: 5217

1KFT

Solution Structure and DNA Binding Properties of the C-Terminal Domain of UvrC from E. coli

Authors

Singh, S., Folkers, G.E., Bonvin, A.M. J. J., Wechselberger, R., Niztayev, A., Boelens, R., Kaptein, R.

Assembly

UvrC C-terminal domain

Entity

1. UvrC C-terminal domain (polymer, Thiol state: not present), 78 monomers, 8562.722 Da Detail

MGSSHHHHHH SSGLVPRGSH MNTSSLETIE GVGPKRRQML LKYMGGLQGL RNASVEEIAK VPGISQGLAE KIFWSLKH

Formula weight

8562.722 Da

Source organism

Escherichia coli

Exptl. method

NMR

Refine. method

A simulated annealing protocol in Cartesian space was used.

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 88.5 %, Completeness: 81.4 %, Completeness (bb): 83.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	81.4 % (727 of 893)	82.9 % (387 of 467)	78.8 % (272 of 345)	84.0 % (68 of 81)
Backbone	83.3 % (385 of 462)	82.2 % (134 of 163)	83.9 % (188 of 224)	84.0 % (63 of 75)
Sidechain	80.4 % (401 of 499)	83.2 % (253 of 304)	75.7 % (143 of 189)	83.3 % (5 of 6)
Aromatic	38.7 % (24 of 62)	64.5 % (20 of 31)	10.0 % (3 of 30)	100.0 % (1 of 1)
Methyl	95.9 % (71 of 74)	100.0 % (37 of 37)	91.9 % (34 of 37)

1. UV Repair protein C

MGSSHHHHHH SSGLVPRGSH MNTSSLETIE GVGPKRRQML LKYMGGLQGL RNASVEEIAK VPGISQGLAE KIFWSLKH

Show sample condition

Sample #1

Temperature 300 (±1.0) K, pH 6.8 (±0.2), Details A complete protease inhibitor was added to the sample.

#	Name	Isotope labeling	Concentration
1	UV Repair protein C	[U-15N]	1.0 mM
2	NaCl		300 mM
3	sodium phosphate buffer		50 mM

Sample #2

Temperature 300 (±1.0) K, pH 6.8 (±0.2), Details A complete protease inhibitor was added to the sample.

#	Name	Isotope labeling	Concentration
4	UV Repair protein C	[U-15N; U-13C]	1.0 mM
5	NaCl		300 mM
6	sodium phosphate buffer		50 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
¹H	H2O	protons	4.75 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Referencing offset: 0.02 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
¹H	H2O	protons	4.75 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Referencing offset: 0.02 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
¹H	H2O	protons	4.75 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Referencing offset: 0.08 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
¹H	H2O	protons	4.75 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Referencing offset: -0.16 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 22 models in PDB: 1KFT, Strand ID: A Detail

Release date

2003-01-13

Citation

Solution structure and DNA-binding properties of the C-terminal domain of UvrC from E.coli
Singh, S., Folkers, G.E., Bonvin, A.M., Boelens, R., Wechselberger, R., Niztayev, A., Kaptein, R.
EMBO J. (2002), 21, 6257-6266, PubMed 12426397 , DOI 10.2210/pdb1kft/pdb , Abstract

Related entities 1. UvrC C-terminal domain, : 1 : 91 entities Detail

Experiments performed 10 experiments Detail

1 HNCA

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker AMX (600 MHz)

#3 Model Varian INOVA (750 MHz)

Sample #1

Temperature 300 (±1.0) K, pH 6.8 (±0.2), Details A complete protease inhibitor was added to the sample.

#	Name	Isotope labeling	Concentration
1	UV Repair protein C	[U-15N]	1.0 mM
2	NaCl		300 mM
3	sodium phosphate buffer		50 mM

Sample #2

Temperature 300 (±1.0) K, pH 6.8 (±0.2), Details A complete protease inhibitor was added to the sample.

#	Name	Isotope labeling	Concentration
4	UV Repair protein C	[U-15N; U-13C]	1.0 mM
5	NaCl		300 mM
6	sodium phosphate buffer		50 mM

2 HNCO

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker AMX (600 MHz)

#3 Model Varian INOVA (750 MHz)

Sample #1

Temperature 300 (±1.0) K, pH 6.8 (±0.2), Details A complete protease inhibitor was added to the sample.

#	Name	Isotope labeling	Concentration
1	UV Repair protein C	[U-15N]	1.0 mM
2	NaCl		300 mM
3	sodium phosphate buffer		50 mM

Sample #2

Temperature 300 (±1.0) K, pH 6.8 (±0.2), Details A complete protease inhibitor was added to the sample.

#	Name	Isotope labeling	Concentration
4	UV Repair protein C	[U-15N; U-13C]	1.0 mM
5	NaCl		300 mM
6	sodium phosphate buffer		50 mM

3 HN(CO)CA

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker AMX (600 MHz)

#3 Model Varian INOVA (750 MHz)

Sample #1

Temperature 300 (±1.0) K, pH 6.8 (±0.2), Details A complete protease inhibitor was added to the sample.

#	Name	Isotope labeling	Concentration
1	UV Repair protein C	[U-15N]	1.0 mM
2	NaCl		300 mM
3	sodium phosphate buffer		50 mM

Sample #2

Temperature 300 (±1.0) K, pH 6.8 (±0.2), Details A complete protease inhibitor was added to the sample.

#	Name	Isotope labeling	Concentration
4	UV Repair protein C	[U-15N; U-13C]	1.0 mM
5	NaCl		300 mM
6	sodium phosphate buffer		50 mM

4 CBCANH

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker AMX (600 MHz)

#3 Model Varian INOVA (750 MHz)

Sample #1

Temperature 300 (±1.0) K, pH 6.8 (±0.2), Details A complete protease inhibitor was added to the sample.

#	Name	Isotope labeling	Concentration
1	UV Repair protein C	[U-15N]	1.0 mM
2	NaCl		300 mM
3	sodium phosphate buffer		50 mM

Sample #2

Temperature 300 (±1.0) K, pH 6.8 (±0.2), Details A complete protease inhibitor was added to the sample.

#	Name	Isotope labeling	Concentration
4	UV Repair protein C	[U-15N; U-13C]	1.0 mM
5	NaCl		300 mM
6	sodium phosphate buffer		50 mM

5 1H-15N TOCSY HSQC

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker AMX (600 MHz)

#3 Model Varian INOVA (750 MHz)

Sample #1

Temperature 300 (±1.0) K, pH 6.8 (±0.2), Details A complete protease inhibitor was added to the sample.

#	Name	Isotope labeling	Concentration
1	UV Repair protein C	[U-15N]	1.0 mM
2	NaCl		300 mM
3	sodium phosphate buffer		50 mM

Sample #2

Temperature 300 (±1.0) K, pH 6.8 (±0.2), Details A complete protease inhibitor was added to the sample.

#	Name	Isotope labeling	Concentration
4	UV Repair protein C	[U-15N; U-13C]	1.0 mM
5	NaCl		300 mM
6	sodium phosphate buffer		50 mM

6 1H-15N NOESY HSQC

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker AMX (600 MHz)

#3 Model Varian INOVA (750 MHz)

Sample #1

Temperature 300 (±1.0) K, pH 6.8 (±0.2), Details A complete protease inhibitor was added to the sample.

#	Name	Isotope labeling	Concentration
1	UV Repair protein C	[U-15N]	1.0 mM
2	NaCl		300 mM
3	sodium phosphate buffer		50 mM

Sample #2

Temperature 300 (±1.0) K, pH 6.8 (±0.2), Details A complete protease inhibitor was added to the sample.

#	Name	Isotope labeling	Concentration
4	UV Repair protein C	[U-15N; U-13C]	1.0 mM
5	NaCl		300 mM
6	sodium phosphate buffer		50 mM

7 1H-13C-H(C)CH TOCSY

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker AMX (600 MHz)

#3 Model Varian INOVA (750 MHz)

Sample #1

Temperature 300 (±1.0) K, pH 6.8 (±0.2), Details A complete protease inhibitor was added to the sample.

#	Name	Isotope labeling	Concentration
1	UV Repair protein C	[U-15N]	1.0 mM
2	NaCl		300 mM
3	sodium phosphate buffer		50 mM

Sample #2

Temperature 300 (±1.0) K, pH 6.8 (±0.2), Details A complete protease inhibitor was added to the sample.

#	Name	Isotope labeling	Concentration
4	UV Repair protein C	[U-15N; U-13C]	1.0 mM
5	NaCl		300 mM
6	sodium phosphate buffer		50 mM

8 1H-13C-(H)CCH TOCSY

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker AMX (600 MHz)

#3 Model Varian INOVA (750 MHz)

Sample #1

Temperature 300 (±1.0) K, pH 6.8 (±0.2), Details A complete protease inhibitor was added to the sample.

#	Name	Isotope labeling	Concentration
1	UV Repair protein C	[U-15N]	1.0 mM
2	NaCl		300 mM
3	sodium phosphate buffer		50 mM

Sample #2

Temperature 300 (±1.0) K, pH 6.8 (±0.2), Details A complete protease inhibitor was added to the sample.

#	Name	Isotope labeling	Concentration
4	UV Repair protein C	[U-15N; U-13C]	1.0 mM
5	NaCl		300 mM
6	sodium phosphate buffer		50 mM

9 1H-15N NOESY HSQC

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker AMX (600 MHz)

#3 Model Varian INOVA (750 MHz)

Sample #1

Temperature 300 (±1.0) K, pH 6.8 (±0.2), Details A complete protease inhibitor was added to the sample.

#	Name	Isotope labeling	Concentration
1	UV Repair protein C	[U-15N]	1.0 mM
2	NaCl		300 mM
3	sodium phosphate buffer		50 mM

Sample #2

Temperature 300 (±1.0) K, pH 6.8 (±0.2), Details A complete protease inhibitor was added to the sample.

#	Name	Isotope labeling	Concentration
4	UV Repair protein C	[U-15N; U-13C]	1.0 mM
5	NaCl		300 mM
6	sodium phosphate buffer		50 mM

10 2D-NOESY

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker AMX (600 MHz)

#3 Model Varian INOVA (750 MHz)

Sample #1

Temperature 300 (±1.0) K, pH 6.8 (±0.2), Details A complete protease inhibitor was added to the sample.

#	Name	Isotope labeling	Concentration
1	UV Repair protein C	[U-15N]	1.0 mM
2	NaCl		300 mM
3	sodium phosphate buffer		50 mM

Sample #2

Temperature 300 (±1.0) K, pH 6.8 (±0.2), Details A complete protease inhibitor was added to the sample.

#	Name	Isotope labeling	Concentration
4	UV Repair protein C	[U-15N; U-13C]	1.0 mM
5	NaCl		300 mM
6	sodium phosphate buffer		50 mM

nullKeywords DNA binding domain, Helix-hairpin-helix motif, NER Protein, UvrC CTD

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Found 1 Document (1.12 seconds)

BMRB: 5217

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Chemical shift reference

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Chemical shift reference

Related entities 1. UvrC C-terminal domain, : 1 : 91 entities Detail

Experiments performed 10 experiments Detail

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