BMRB: 5220

Solution Structure and dynamics of melanoma inhibitory activity protein

Authors

Lougheed, J.C., Domaille, P.J., Handel, T.M.

Assembly

Melanoma Inhibitory Activity Protein

Entity

1. Melanoma Inhibitory Activity Protein (polymer, Thiol state: all disulfide bound), 108 monomers, 12240.99 Da Detail

MGPMPKLADR KLCADQECSH PISMAVALQD YMAPDCRFLT IHRGQVVYVF SKLKGRGRLF WGGSVQGDYY GDLAARLGYF PSSIVREDQT LKPGKVDVKT DKWDFYCQ

Formula weight

12240.99 Da

Entity Connection

disulfide 2 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS13:SG	1:CYS18:SG
2	disulfide	sing	1:CYS36:SG	1:CYS107:SG

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

Restrained molecular dynamics-simulated annealing coupled with the ambiguous restraints for iterative assignment (ARIA) extension of Nilges (M. Nilges, J. Mol. Biol. 245, 645-660, 1995).

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 91.4 %, Completeness (bb): 85.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	91.4 % (1164 of 1273)	98.3 % (654 of 665)	80.3 % (400 of 498)	100.0 % (110 of 110)
Backbone	85.1 % (541 of 636)	99.5 % (219 of 220)	70.1 % (220 of 314)	100.0 % (102 of 102)
Sidechain	98.1 % (721 of 735)	97.8 % (435 of 445)	98.6 % (278 of 282)	100.0 % (8 of 8)
Aromatic	98.5 % (128 of 130)	98.5 % (64 of 65)	98.4 % (62 of 63)	100.0 % (2 of 2)
Methyl	100.0 % (100 of 100)	100.0 % (50 of 50)	100.0 % (50 of 50)

1. melanoma inhibitory activity protein

MGPMPKLADR KLCADQECSH PISMAVALQD YMAPDCRFLT IHRGQVVYVF SKLKGRGRLF WGGSVQGDYY GDLAARLGYF PSSIVREDQT LKPGKVDVKT DKWDFYCQ

Show sample condition

Sample

Pressure 1 atm, Temperature 298 (±0.5) K, pH 4.33 (±.03)

#	Name	Isotope labeling	Type	Concentration
1	melanoma inhibitory activity protein	[U-13C; U-15N]		0.7 ~ 0.9 mM
2	sodium acetate	[U-2H]		20 mM
3	azide			0.02 %

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LACS Plot; CA

Referencing offset: -0.36 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.36 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.06 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1K0X, Strand ID: A Detail

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Release date

2001-11-28

Citation

Solution structure and dynamics of melanoma inhibitory activity protein
Lougheed, J.C., Domaille, P.J., Handel, T.M.
J. Biomol. NMR (2002), 22, 211-223, PubMed 11991352 , DOI 10.2210/pdb1k0x/pdb , Abstract

Related entities 1. Melanoma Inhibitory Activity Protein, : 1 : 2 : 3 : 27 entities Detail

Interaction partners 1. Melanoma Inhibitory Activity Protein, : 1 interactors Detail

Experiments performed 12 experiments Detail