BMRB: 52208

Backbone chemical shift assignments for HMG-D Y12F mutant free protein

Authors

Yang, J., Hill, G.R., Neuhaus, D.

Assembly

HMG-D Y12F free protein

Entity

1. HMG-D Y12F free protein (polymer, Thiol state: not present), 112 monomers, 12399.72 Da Detail

MSDKPKRPLS AFMLWLNSAR ESIKRENPGI KVTEVAKRGG ELWRAMKDKS EWEAKAAKAK DDYDRAVKEF EANGGSSAAN GGGAKKRAKP AKKVAKKSKK EESDEDDDDE SE

Formula weight

12399.72 Da

Source organism

Drosophila melanogaster

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chemcal Shifts

¹H: 71, ¹³C: 144, ¹⁵N: 71 assignments Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

ID	Entity ID	Seq ID	Comp ID	Atom ID	Atom type	Val	Val err	Ambig. code
1	1	6	LYS	H	H	8.467	0.02	1
2	1	6	LYS	N	N	122.693	0.10	1
3	1	8	PRO	CA	C	62.471	0.10	1
4	1	8	PRO	CB	C	32.254	0.10	1
5	1	9	LEU	H	H	8.022	0.02	1
6	1	9	LEU	CA	C	54.877	0.10	1
7	1	9	LEU	CB	C	43.791	0.10	1
8	1	9	LEU	N	N	121.775	0.10	1
9	1	10	SER	H	H	7.966	0.02	1
10	1	10	SER	CA	C	57.145	0.10	1
11	1	10	SER	CB	C	66.003	0.10	1
12	1	10	SER	N	N	114.871	0.10	1
13	1	11	ALA	H	H	8.948	0.02	1
14	1	11	ALA	CA	C	56.652	0.10	1
15	1	11	ALA	CB	C	18.913	0.10	1
16	1	11	ALA	N	N	122.298	0.10	1
17	1	12	PHE	H	H	8.150	0.02	1
18	1	12	PHE	CA	C	59.622	0.10	1
19	1	12	PHE	CB	C	39.330	0.10	1
20	1	12	PHE	N	N	114.859	0.10	1
21	1	13	MET	H	H	7.438	0.02	1
22	1	13	MET	CA	C	57.037	0.10	1
23	1	13	MET	CB	C	31.864	0.10	1
24	1	13	MET	N	N	116.857	0.10	1
25	1	14	LEU	H	H	8.520	0.02	1
26	1	14	LEU	CA	C	58.523	0.10	1
27	1	14	LEU	CB	C	42.908	0.10	1
28	1	14	LEU	N	N	120.818	0.10	1
29	1	15	TRP	H	H	8.000	0.02	1
30	1	15	TRP	CA	C	61.640	0.10	1
31	1	15	TRP	N	N	122.297	0.10	1
32	1	16	LEU	H	H	8.981	0.02	1
33	1	16	LEU	CA	C	57.714	0.10	1
34	1	16	LEU	CB	C	40.506	0.10	1
35	1	16	LEU	N	N	123.081	0.10	1
36	1	17	ASN	H	H	7.812	0.02	1
37	1	17	ASN	CA	C	55.845	0.10	1
38	1	17	ASN	CB	C	37.672	0.10	1
39	1	17	ASN	N	N	114.827	0.10	1
40	1	18	SER	H	H	7.171	0.02	1
41	1	18	SER	CA	C	60.714	0.10	1
42	1	18	SER	CB	C	64.029	0.10	1
43	1	18	SER	N	N	116.227	0.10	1
44	1	19	ALA	H	H	7.400	0.02	1
45	1	19	ALA	CA	C	52.940	0.10	1
46	1	19	ALA	CB	C	20.067	0.10	1
47	1	19	ALA	N	N	124.261	0.10	1
48	1	20	ARG	H	H	7.705	0.02	1
49	1	20	ARG	CA	C	61.654	0.10	1
50	1	20	ARG	CB	C	30.593	0.10	1
51	1	20	ARG	N	N	119.749	0.10	1
52	1	21	GLU	H	H	8.392	0.02	1
53	1	21	GLU	CA	C	59.958	0.10	1
54	1	21	GLU	CB	C	28.973	0.10	1
55	1	21	GLU	N	N	116.778	0.10	1
56	1	22	SER	H	H	8.050	0.02	1
57	1	22	SER	CA	C	62.163	0.10	1
58	1	22	SER	CB	C	62.986	0.10	1
59	1	22	SER	N	N	117.219	0.10	1
60	1	23	ILE	H	H	8.096	0.02	1
61	1	23	ILE	CA	C	65.978	0.10	1
62	1	23	ILE	CB	C	38.767	0.10	1
63	1	23	ILE	N	N	122.945	0.10	1
64	1	24	LYS	H	H	7.978	0.02	1
65	1	24	LYS	CA	C	60.059	0.10	1
66	1	24	LYS	CB	C	32.577	0.10	1
67	1	24	LYS	N	N	118.576	0.10	1
68	1	25	ARG	H	H	7.932	0.02	1
69	1	25	ARG	CA	C	59.336	0.10	1
70	1	25	ARG	CB	C	30.726	0.10	1
71	1	25	ARG	N	N	118.364	0.10	1
72	1	26	GLU	H	H	7.805	0.02	1
73	1	26	GLU	CA	C	57.552	0.10	1
74	1	26	GLU	CB	C	30.741	0.10	1
75	1	26	GLU	N	N	114.914	0.10	1
76	1	27	ASN	H	H	7.273	0.02	1
77	1	27	ASN	CA	C	51.296	0.10	1
78	1	27	ASN	CB	C	40.969	0.10	1
79	1	27	ASN	N	N	116.492	0.10	1
80	1	28	PRO	CA	C	64.562	0.10	1
81	1	28	PRO	CB	C	31.937	0.10	1
82	1	29	GLY	H	H	8.702	0.02	1
83	1	29	GLY	CA	C	45.657	0.10	1
84	1	29	GLY	N	N	110.975	0.10	1
85	1	30	ILE	H	H	7.783	0.02	1
86	1	30	ILE	CA	C	62.473	0.10	1
87	1	30	ILE	CB	C	38.774	0.10	1
88	1	30	ILE	N	N	120.800	0.10	1
89	1	31	LYS	H	H	8.571	0.02	1
90	1	31	LYS	CA	C	56.272	0.10	1
91	1	31	LYS	CB	C	34.239	0.10	1
92	1	31	LYS	N	N	125.693	0.10	1
93	1	32	VAL	H	H	8.758	0.02	1
94	1	32	VAL	CA	C	66.823	0.10	1
95	1	32	VAL	CB	C	31.969	0.10	1
96	1	32	VAL	N	N	121.076	0.10	1
97	1	33	THR	H	H	7.549	0.02	1
98	1	33	THR	CA	C	64.055	0.10	1
99	1	33	THR	CB	C	68.645	0.10	1
100	1	33	THR	N	N	108.867	0.10	1
101	1	34	GLU	H	H	7.584	0.02	1
102	1	34	GLU	CA	C	57.374	0.10	1
103	1	34	GLU	CB	C	30.733	0.10	1
104	1	34	GLU	N	N	120.573	0.10	1
105	1	35	VAL	H	H	7.623	0.02	1
106	1	35	VAL	CA	C	67.613	0.10	1
107	1	35	VAL	CB	C	31.921	0.10	1
108	1	35	VAL	N	N	122.264	0.10	1
109	1	38	ARG	CA	C	58.089	0.10	1
110	1	38	ARG	CB	C	29.680	0.10	1
111	1	39	GLY	H	H	9.294	0.02	1
112	1	39	GLY	CA	C	48.060	0.10	1
113	1	39	GLY	N	N	106.286	0.10	1
114	1	40	GLY	H	H	8.628	0.02	1
115	1	40	GLY	CA	C	48.309	0.10	1
116	1	40	GLY	N	N	109.427	0.10	1
117	1	41	GLU	H	H	7.533	0.02	1
118	1	41	GLU	CA	C	59.619	0.10	1
119	1	41	GLU	CB	C	30.062	0.10	1
120	1	41	GLU	N	N	121.030	0.10	1
121	1	42	LEU	H	H	8.017	0.02	1
122	1	42	LEU	CA	C	58.128	0.10	1
123	1	42	LEU	CB	C	42.912	0.10	1
124	1	42	LEU	N	N	120.982	0.10	1
125	1	43	TRP	H	H	8.906	0.02	1
126	1	43	TRP	CA	C	59.721	0.10	1
127	1	43	TRP	CB	C	30.927	0.10	1
128	1	43	TRP	N	N	121.355	0.10	1
129	1	44	ARG	H	H	7.926	0.02	1
130	1	44	ARG	CA	C	59.653	0.10	1
131	1	44	ARG	CB	C	30.463	0.10	1
132	1	44	ARG	N	N	116.350	0.10	1
133	1	45	ALA	H	H	7.018	0.02	1
134	1	45	ALA	CA	C	52.514	0.10	1
135	1	45	ALA	CB	C	19.699	0.10	1
136	1	45	ALA	N	N	117.872	0.10	1
137	1	46	MET	H	H	7.071	0.02	1
138	1	46	MET	CA	C	56.825	0.10	1
139	1	46	MET	CB	C	33.366	0.10	1
140	1	46	MET	N	N	120.792	0.10	1
141	1	47	LYS	H	H	8.493	0.02	1
142	1	47	LYS	CA	C	58.858	0.10	1
143	1	47	LYS	CB	C	32.915	0.10	1
144	1	47	LYS	N	N	126.637	0.10	1
145	1	48	ASP	H	H	8.133	0.02	1
146	1	48	ASP	CA	C	53.177	0.10	1
147	1	48	ASP	CB	C	41.423	0.10	1
148	1	48	ASP	N	N	116.682	0.10	1
149	1	49	LYS	H	H	8.745	0.02	1
150	1	49	LYS	CA	C	56.515	0.10	1
151	1	49	LYS	CB	C	33.410	0.10	1
152	1	49	LYS	N	N	125.377	0.10	1
153	1	50	SER	H	H	8.447	0.02	1
154	1	50	SER	N	N	117.431	0.10	1
155	1	51	GLU	H	H	9.026	0.02	1
156	1	51	GLU	CA	C	59.765	0.10	1
157	1	51	GLU	CB	C	29.755	0.10	1
158	1	51	GLU	N	N	124.247	0.10	1
159	1	52	TRP	H	H	6.930	0.02	1
160	1	52	TRP	CA	C	59.429	0.10	1
161	1	52	TRP	CB	C	30.365	0.10	1
162	1	52	TRP	N	N	120.951	0.10	1
163	1	53	GLU	H	H	8.493	0.02	1
164	1	53	GLU	CA	C	60.119	0.10	1
165	1	53	GLU	CB	C	29.743	0.10	1
166	1	53	GLU	N	N	119.474	0.10	1
167	1	54	ALA	H	H	8.351	0.02	1
168	1	54	ALA	CA	C	55.631	0.10	1
169	1	54	ALA	CB	C	18.192	0.10	1
170	1	54	ALA	N	N	122.645	0.10	1
171	1	55	LYS	H	H	7.783	0.02	1
172	1	55	LYS	CA	C	60.422	0.10	1
173	1	55	LYS	CB	C	33.702	0.10	1
174	1	55	LYS	N	N	119.472	0.10	1
175	1	56	ALA	H	H	8.528	0.02	1
176	1	56	ALA	CA	C	55.442	0.10	1
177	1	56	ALA	CB	C	18.450	0.10	1
178	1	56	ALA	N	N	123.183	0.10	1
179	1	57	ALA	H	H	8.267	0.02	1
180	1	57	ALA	CA	C	55.627	0.10	1
181	1	57	ALA	CB	C	18.224	0.10	1
182	1	57	ALA	N	N	123.011	0.10	1
183	1	58	LYS	H	H	7.839	0.02	1
184	1	58	LYS	CA	C	59.249	0.10	1
185	1	58	LYS	CB	C	32.549	0.10	1
186	1	58	LYS	N	N	119.774	0.10	1
187	1	59	ALA	H	H	7.954	0.02	1
188	1	59	ALA	CA	C	55.347	0.10	1
189	1	59	ALA	CB	C	18.354	0.10	1
190	1	59	ALA	N	N	120.250	0.10	1
191	1	60	LYS	H	H	8.178	0.02	1
192	1	60	LYS	CA	C	60.017	0.10	1
193	1	60	LYS	CB	C	32.348	0.10	1
194	1	60	LYS	N	N	121.200	0.10	1
195	1	61	ASP	H	H	8.115	0.02	1
196	1	61	ASP	CA	C	58.241	0.10	1
197	1	61	ASP	CB	C	41.141	0.10	1
198	1	61	ASP	N	N	120.959	0.10	1
199	1	62	ASP	H	H	8.599	0.02	1
200	1	62	ASP	CA	C	57.774	0.10	1
201	1	62	ASP	CB	C	40.696	0.10	1
202	1	62	ASP	N	N	119.274	0.10	1
203	1	63	TYR	H	H	8.085	0.02	1
204	1	63	TYR	CA	C	61.727	0.10	1
205	1	63	TYR	CB	C	38.963	0.10	1
206	1	63	TYR	N	N	123.001	0.10	1
207	1	64	ASP	H	H	8.866	0.02	1
208	1	64	ASP	CA	C	57.756	0.10	1
209	1	64	ASP	CB	C	40.421	0.10	1
210	1	64	ASP	N	N	120.233	0.10	1
211	1	65	ARG	H	H	7.880	0.02	1
212	1	65	ARG	CA	C	59.556	0.10	1
213	1	65	ARG	CB	C	30.583	0.10	1
214	1	65	ARG	N	N	118.769	0.10	1
215	1	66	ALA	H	H	7.937	0.02	1
216	1	66	ALA	CA	C	55.066	0.10	1
217	1	66	ALA	CB	C	18.912	0.10	1
218	1	66	ALA	N	N	122.763	0.10	1
219	1	67	VAL	H	H	8.556	0.02	1
220	1	67	VAL	CA	C	66.274	0.10	1
221	1	67	VAL	CB	C	32.027	0.10	1
222	1	67	VAL	N	N	118.872	0.10	1
223	1	68	LYS	H	H	7.506	0.02	1
224	1	68	LYS	CA	C	58.971	0.10	1
225	1	68	LYS	CB	C	32.668	0.10	1
226	1	68	LYS	N	N	120.285	0.10	1
227	1	69	GLU	H	H	7.832	0.02	1
228	1	69	GLU	CA	C	58.483	0.10	1
229	1	69	GLU	CB	C	30.188	0.10	1
230	1	69	GLU	N	N	117.890	0.10	1
231	1	70	PHE	H	H	8.028	0.02	1
232	1	70	PHE	CA	C	60.991	0.10	1
233	1	70	PHE	CB	C	39.896	0.10	1
234	1	70	PHE	N	N	119.679	0.10	1
235	1	71	GLU	H	H	8.509	0.02	1
236	1	71	GLU	CA	C	58.857	0.10	1
237	1	71	GLU	CB	C	29.967	0.10	1
238	1	71	GLU	N	N	118.881	0.10	1
239	1	72	ALA	H	H	7.896	0.02	1
240	1	72	ALA	CA	C	54.420	0.10	1
241	1	72	ALA	CB	C	18.935	0.10	1
242	1	72	ALA	N	N	122.381	0.10	1
243	1	73	ASN	H	H	7.800	0.02	1
244	1	73	ASN	CA	C	53.707	0.10	1
245	1	73	ASN	CB	C	39.403	0.10	1
246	1	73	ASN	N	N	114.923	0.10	1
247	1	74	GLY	H	H	7.934	0.02	1
248	1	74	GLY	CA	C	46.005	0.10	1
249	1	74	GLY	N	N	107.878	0.10	1
250	1	75	GLY	H	H	8.046	0.02	1
251	1	75	GLY	CA	C	45.724	0.10	1
252	1	75	GLY	N	N	108.494	0.10	1
253	1	76	SER	H	H	8.236	0.02	1
254	1	76	SER	CA	C	58.787	0.10	1
255	1	76	SER	CB	C	64.444	0.10	1
256	1	76	SER	N	N	115.614	0.10	1
257	1	77	SER	H	H	8.429	0.02	1
258	1	77	SER	CA	C	58.880	0.10	1
259	1	77	SER	CB	C	64.322	0.10	1
260	1	77	SER	N	N	117.794	0.10	1
261	1	90	PRO	CA	C	63.361	0.10	1
262	1	90	PRO	CB	C	32.527	0.10	1
263	1	91	ALA	H	H	8.415	0.02	1
264	1	91	ALA	CA	C	52.661	0.10	1
265	1	91	ALA	CB	C	19.729	0.10	1
266	1	91	ALA	N	N	124.629	0.10	1
267	1	94	VAL	CA	C	62.407	0.10	1
268	1	94	VAL	CB	C	33.078	0.10	1
269	1	95	ALA	H	H	8.387	0.02	1
270	1	95	ALA	CA	C	52.687	0.10	1
271	1	95	ALA	CB	C	19.727	0.10	1
272	1	95	ALA	N	N	127.960	0.10	1
273	1	100	LYS	CA	C	57.227	0.10	1
274	1	100	LYS	CB	C	33.245	0.10	1
275	1	101	GLU	H	H	8.468	0.02	1
276	1	101	GLU	CA	C	57.255	0.10	1
277	1	101	GLU	CB	C	30.595	0.10	1
278	1	101	GLU	N	N	120.850	0.10	1
279	1	102	GLU	H	H	8.426	0.02	1
280	1	102	GLU	CA	C	57.350	0.10	1
281	1	102	GLU	CB	C	30.491	0.10	1
282	1	102	GLU	N	N	120.271	0.10	1
283	1	103	SER	H	H	8.167	0.02	1
284	1	103	SER	CA	C	58.625	0.10	1
285	1	103	SER	CB	C	64.464	0.10	1
286	1	103	SER	N	N	115.010	0.10	1

Release date

2023-11-14

Citation

A Single Interfacial Point Mutation Rescues Solution Structure Determination of the Complex of HMG-D with a DNA Bulge
Hill, G., Yang, J.C., Easton, L., Cerdan, R., McLaughlin, S., Stott, K., Travers, A., Neuhaus, D.
Chembiochem. (2024), e202400395-e202400395, PubMed 39145407 , DOI 10.1002/cbic.202400395 , Abstract

Related entities 1. HMG-D Y12F free protein, : 2 : 24 entities Detail

Interaction partners 1. HMG-D Y12F free protein, : 2 interactors Detail

Experiments performed 4 experiments Detail

Chemical shift validation 3 contents Detail