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BMRB: 5232

1KXL

1H, 13C, and 15N resonance assignments of the DNA-binding domain of the essential protein Cdc13 complexed with single-stranded telomeric DNA

Authors

Mitton-Fry, R.M., Wuttke, D.S.

Assembly

Cdc13 DNA-binding domain /telomeric ssDNA complex

Entity

1. Cdc13 DNA-binding domain (polymer, Thiol state: all free), 199 monomers, 23541.82 Da Detail

MRMSKMARKD PTIEFCQLGL DTFETKYITM FGMLVSCSFD KPAFISFVFS DFTKNDIVQN YLYDRYLIDY ENKLELNEGF KAIMYKNQFE TFDSKLRKIF NNGLRDLQNG RDENLSQYGI VCKMNIKVKM YNGKLNAIVR ECEPVPHSQI SSIASPSQCE HLRLFYQRAF KRIGESAISR YFEEYRRFFP IHRNGSHLA

2. telomeric single-stranded DNA 11mer (polymer, Thiol state: not present), 11 monomers, 3539.226 Da Detail

GTGTGGGTGT G

Total weight

27081.047 Da

Max. entity weight

23541.82 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

NMR

Refine. method

distance geometry, simulated annealing, molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 89.0 %, Completeness: 81.7 %, Completeness (bb): 87.5 % Detail

Polymer type: polypeptide(L) polydeoxyribonucleotide

	Total	¹H	¹³C	¹⁵N
All	81.7 % (2103 of 2573)	76.9 % (1078 of 1402)	87.3 % (835 of 957)	88.8 % (190 of 214)
Backbone	87.5 % (1101 of 1259)	77.4 % (370 of 478)	93.5 % (550 of 588)	93.8 % (181 of 193)
Sidechain	78.5 % (1180 of 1504)	76.6 % (708 of 924)	83.0 % (464 of 559)	38.1 % (8 of 21)
Aromatic	53.4 % (158 of 296)	50.9 % (81 of 159)	56.2 % (77 of 137)
Methyl	95.0 % (171 of 180)	92.4 % (85 of 92)	97.7 % (86 of 88)

1. Cdc13 DNA-binding domain

2. telomeric single-stranded DNA 11mer

GTGTGGGTGT G

Show sample condition

Sample #1

Temperature 303 (±1.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	Cdc13 DNA-binding domain	[U-13C; U-15N]	0.7 ~ 1.1 mM
2	telomeric single-stranded DNA 11mer		0.8 ~ 1.2 mM
3	imidazole	[U-2H]	50 mM
4	sodium chloride		150 mM
5	sodium sulfate		100 mM
6	sodium azide		0.02 %
7	DTT	[U-2H]	2 mM

Sample #2

Temperature 303 (±1.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
8	Cdc13 DNA-binding domain	[U-15N]	1.5 mM
9	telomeric single-stranded DNA 11mer		1.7 mM
10	imidazole	[U-2H]	50 mM
11	sodium chloride		150 mM
12	sodium sulfate		100 mM
13	sodium azide		0.02 %
14	DTT	[U-2H]	2 mM

Sample #3

Temperature 303 (±1.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
15	Cdc13 DNA-binding domain		1.1 mM
16	telomeric single-stranded DNA 11mer		1.2 mM
17	imidazole	[U-2H]	50 mM
18	sodium chloride		150 mM
19	sodium sulfate		100 mM
20	sodium azide		0.02 %
21	DTT	[U-2H]	2 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	H2O	protons	4.72 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

Referencing offset: -0.19 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	H2O	protons	4.72 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

Referencing offset: -0.19 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	H2O	protons	4.72 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

Referencing offset: -0.03 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	H2O	protons	4.72 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

Referencing offset: 0.02 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 1KXL, Strand ID: A Detail

Release date

2002-08-21

Citation

1H, 13C and 15N resonance assignments of the DNA-binding domain of the essential protein Cdc13 complexed with single-stranded telomeric DNA
Mitton-Fry, R.M., Wuttke, D.S.
J. Biomol. NMR (2002), 22, 379-380, PubMed 12018489 , DOI: , Abstract

Related entities 1. Cdc13 DNA-binding domain, : 1 : 1 : 2 entities Detail

Experiments performed 20 experiments Detail

1 2D 1H-1H NOESY

Instrument

#1 Model Varian UnityInova (500 MHz)

#2 Model Varian UnityInova (600 MHz)

#3 Model Bruker DRX (800 MHz)

Sample #1

Temperature 303 (±1.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	Cdc13 DNA-binding domain	[U-13C; U-15N]	0.7 ~ 1.1 mM
2	telomeric single-stranded DNA 11mer		0.8 ~ 1.2 mM
3	imidazole	[U-2H]	50 mM
4	sodium chloride		150 mM
5	sodium sulfate		100 mM
6	sodium azide		0.02 %
7	DTT	[U-2H]	2 mM

Sample #2

Temperature 303 (±1.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
8	Cdc13 DNA-binding domain	[U-15N]	1.5 mM
9	telomeric single-stranded DNA 11mer		1.7 mM
10	imidazole	[U-2H]	50 mM
11	sodium chloride		150 mM
12	sodium sulfate		100 mM
13	sodium azide		0.02 %
14	DTT	[U-2H]	2 mM

Sample #3

Temperature 303 (±1.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
15	Cdc13 DNA-binding domain		1.1 mM
16	telomeric single-stranded DNA 11mer		1.2 mM
17	imidazole	[U-2H]	50 mM
18	sodium chloride		150 mM
19	sodium sulfate		100 mM
20	sodium azide		0.02 %
21	DTT	[U-2H]	2 mM

2 2D 1H-13C CT-HSQC

Instrument

#1 Model Varian UnityInova (500 MHz)

#2 Model Varian UnityInova (600 MHz)

#3 Model Bruker DRX (800 MHz)

Sample #1

Temperature 303 (±1.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	Cdc13 DNA-binding domain	[U-13C; U-15N]	0.7 ~ 1.1 mM
2	telomeric single-stranded DNA 11mer		0.8 ~ 1.2 mM
3	imidazole	[U-2H]	50 mM
4	sodium chloride		150 mM
5	sodium sulfate		100 mM
6	sodium azide		0.02 %
7	DTT	[U-2H]	2 mM

Sample #2

Temperature 303 (±1.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
8	Cdc13 DNA-binding domain	[U-15N]	1.5 mM
9	telomeric single-stranded DNA 11mer		1.7 mM
10	imidazole	[U-2H]	50 mM
11	sodium chloride		150 mM
12	sodium sulfate		100 mM
13	sodium azide		0.02 %
14	DTT	[U-2H]	2 mM

Sample #3

Temperature 303 (±1.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
15	Cdc13 DNA-binding domain		1.1 mM
16	telomeric single-stranded DNA 11mer		1.2 mM
17	imidazole	[U-2H]	50 mM
18	sodium chloride		150 mM
19	sodium sulfate		100 mM
20	sodium azide		0.02 %
21	DTT	[U-2H]	2 mM

3 2D 1H-15N HSQC

Instrument

#1 Model Varian UnityInova (500 MHz)

#2 Model Varian UnityInova (600 MHz)

#3 Model Bruker DRX (800 MHz)

Sample #1

Temperature 303 (±1.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	Cdc13 DNA-binding domain	[U-13C; U-15N]	0.7 ~ 1.1 mM
2	telomeric single-stranded DNA 11mer		0.8 ~ 1.2 mM
3	imidazole	[U-2H]	50 mM
4	sodium chloride		150 mM
5	sodium sulfate		100 mM
6	sodium azide		0.02 %
7	DTT	[U-2H]	2 mM

Sample #2

Temperature 303 (±1.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
8	Cdc13 DNA-binding domain	[U-15N]	1.5 mM
9	telomeric single-stranded DNA 11mer		1.7 mM
10	imidazole	[U-2H]	50 mM
11	sodium chloride		150 mM
12	sodium sulfate		100 mM
13	sodium azide		0.02 %
14	DTT	[U-2H]	2 mM

Sample #3

Temperature 303 (±1.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
15	Cdc13 DNA-binding domain		1.1 mM
16	telomeric single-stranded DNA 11mer		1.2 mM
17	imidazole	[U-2H]	50 mM
18	sodium chloride		150 mM
19	sodium sulfate		100 mM
20	sodium azide		0.02 %
21	DTT	[U-2H]	2 mM

4 2D CBHD

Instrument

#1 Model Varian UnityInova (500 MHz)

#2 Model Varian UnityInova (600 MHz)

#3 Model Bruker DRX (800 MHz)

Sample #1

Temperature 303 (±1.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	Cdc13 DNA-binding domain	[U-13C; U-15N]	0.7 ~ 1.1 mM
2	telomeric single-stranded DNA 11mer		0.8 ~ 1.2 mM
3	imidazole	[U-2H]	50 mM
4	sodium chloride		150 mM
5	sodium sulfate		100 mM
6	sodium azide		0.02 %
7	DTT	[U-2H]	2 mM

Sample #2

Temperature 303 (±1.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
8	Cdc13 DNA-binding domain	[U-15N]	1.5 mM
9	telomeric single-stranded DNA 11mer		1.7 mM
10	imidazole	[U-2H]	50 mM
11	sodium chloride		150 mM
12	sodium sulfate		100 mM
13	sodium azide		0.02 %
14	DTT	[U-2H]	2 mM

Sample #3

Temperature 303 (±1.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
15	Cdc13 DNA-binding domain		1.1 mM
16	telomeric single-stranded DNA 11mer		1.2 mM
17	imidazole	[U-2H]	50 mM
18	sodium chloride		150 mM
19	sodium sulfate		100 mM
20	sodium azide		0.02 %
21	DTT	[U-2H]	2 mM

5 2D CBHE

Instrument

#1 Model Varian UnityInova (500 MHz)

#2 Model Varian UnityInova (600 MHz)

#3 Model Bruker DRX (800 MHz)

Sample #1

Temperature 303 (±1.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	Cdc13 DNA-binding domain	[U-13C; U-15N]	0.7 ~ 1.1 mM
2	telomeric single-stranded DNA 11mer		0.8 ~ 1.2 mM
3	imidazole	[U-2H]	50 mM
4	sodium chloride		150 mM
5	sodium sulfate		100 mM
6	sodium azide		0.02 %
7	DTT	[U-2H]	2 mM

Sample #2

Temperature 303 (±1.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
8	Cdc13 DNA-binding domain	[U-15N]	1.5 mM
9	telomeric single-stranded DNA 11mer		1.7 mM
10	imidazole	[U-2H]	50 mM
11	sodium chloride		150 mM
12	sodium sulfate		100 mM
13	sodium azide		0.02 %
14	DTT	[U-2H]	2 mM

Sample #3

Temperature 303 (±1.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
15	Cdc13 DNA-binding domain		1.1 mM
16	telomeric single-stranded DNA 11mer		1.2 mM
17	imidazole	[U-2H]	50 mM
18	sodium chloride		150 mM
19	sodium sulfate		100 mM
20	sodium azide		0.02 %
21	DTT	[U-2H]	2 mM

6 3D 1H-13C-1H HCCH-COSY

Instrument

#1 Model Varian UnityInova (500 MHz)

#2 Model Varian UnityInova (600 MHz)

#3 Model Bruker DRX (800 MHz)

Sample #1

Temperature 303 (±1.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	Cdc13 DNA-binding domain	[U-13C; U-15N]	0.7 ~ 1.1 mM
2	telomeric single-stranded DNA 11mer		0.8 ~ 1.2 mM
3	imidazole	[U-2H]	50 mM
4	sodium chloride		150 mM
5	sodium sulfate		100 mM
6	sodium azide		0.02 %
7	DTT	[U-2H]	2 mM

Sample #2

Temperature 303 (±1.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
8	Cdc13 DNA-binding domain	[U-15N]	1.5 mM
9	telomeric single-stranded DNA 11mer		1.7 mM
10	imidazole	[U-2H]	50 mM
11	sodium chloride		150 mM
12	sodium sulfate		100 mM
13	sodium azide		0.02 %
14	DTT	[U-2H]	2 mM

Sample #3

Temperature 303 (±1.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
15	Cdc13 DNA-binding domain		1.1 mM
16	telomeric single-stranded DNA 11mer		1.2 mM
17	imidazole	[U-2H]	50 mM
18	sodium chloride		150 mM
19	sodium sulfate		100 mM
20	sodium azide		0.02 %
21	DTT	[U-2H]	2 mM

7 3D 1H-1H-15N NOESY

Instrument

#1 Model Varian UnityInova (500 MHz)

#2 Model Varian UnityInova (600 MHz)

#3 Model Bruker DRX (800 MHz)

Sample #1

Temperature 303 (±1.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	Cdc13 DNA-binding domain	[U-13C; U-15N]	0.7 ~ 1.1 mM
2	telomeric single-stranded DNA 11mer		0.8 ~ 1.2 mM
3	imidazole	[U-2H]	50 mM
4	sodium chloride		150 mM
5	sodium sulfate		100 mM
6	sodium azide		0.02 %
7	DTT	[U-2H]	2 mM

Sample #2

Temperature 303 (±1.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
8	Cdc13 DNA-binding domain	[U-15N]	1.5 mM
9	telomeric single-stranded DNA 11mer		1.7 mM
10	imidazole	[U-2H]	50 mM
11	sodium chloride		150 mM
12	sodium sulfate		100 mM
13	sodium azide		0.02 %
14	DTT	[U-2H]	2 mM

Sample #3

Temperature 303 (±1.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
15	Cdc13 DNA-binding domain		1.1 mM
16	telomeric single-stranded DNA 11mer		1.2 mM
17	imidazole	[U-2H]	50 mM
18	sodium chloride		150 mM
19	sodium sulfate		100 mM
20	sodium azide		0.02 %
21	DTT	[U-2H]	2 mM

8 3D 13C-1H-1H NOESY

Instrument

#1 Model Varian UnityInova (500 MHz)

#2 Model Varian UnityInova (600 MHz)

#3 Model Bruker DRX (800 MHz)

Sample #1

Temperature 303 (±1.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	Cdc13 DNA-binding domain	[U-13C; U-15N]	0.7 ~ 1.1 mM
2	telomeric single-stranded DNA 11mer		0.8 ~ 1.2 mM
3	imidazole	[U-2H]	50 mM
4	sodium chloride		150 mM
5	sodium sulfate		100 mM
6	sodium azide		0.02 %
7	DTT	[U-2H]	2 mM

Sample #2

Temperature 303 (±1.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
8	Cdc13 DNA-binding domain	[U-15N]	1.5 mM
9	telomeric single-stranded DNA 11mer		1.7 mM
10	imidazole	[U-2H]	50 mM
11	sodium chloride		150 mM
12	sodium sulfate		100 mM
13	sodium azide		0.02 %
14	DTT	[U-2H]	2 mM

Sample #3

Temperature 303 (±1.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
15	Cdc13 DNA-binding domain		1.1 mM
16	telomeric single-stranded DNA 11mer		1.2 mM
17	imidazole	[U-2H]	50 mM
18	sodium chloride		150 mM
19	sodium sulfate		100 mM
20	sodium azide		0.02 %
21	DTT	[U-2H]	2 mM

9 3D 1H-13C-1H HCCH-TOCSY

Instrument

#1 Model Varian UnityInova (500 MHz)

#2 Model Varian UnityInova (600 MHz)

#3 Model Bruker DRX (800 MHz)

Sample #1

Temperature 303 (±1.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	Cdc13 DNA-binding domain	[U-13C; U-15N]	0.7 ~ 1.1 mM
2	telomeric single-stranded DNA 11mer		0.8 ~ 1.2 mM
3	imidazole	[U-2H]	50 mM
4	sodium chloride		150 mM
5	sodium sulfate		100 mM
6	sodium azide		0.02 %
7	DTT	[U-2H]	2 mM

Sample #2

Temperature 303 (±1.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
8	Cdc13 DNA-binding domain	[U-15N]	1.5 mM
9	telomeric single-stranded DNA 11mer		1.7 mM
10	imidazole	[U-2H]	50 mM
11	sodium chloride		150 mM
12	sodium sulfate		100 mM
13	sodium azide		0.02 %
14	DTT	[U-2H]	2 mM

Sample #3

Temperature 303 (±1.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
15	Cdc13 DNA-binding domain		1.1 mM
16	telomeric single-stranded DNA 11mer		1.2 mM
17	imidazole	[U-2H]	50 mM
18	sodium chloride		150 mM
19	sodium sulfate		100 mM
20	sodium azide		0.02 %
21	DTT	[U-2H]	2 mM

10 3D CBCA(CO)NH

Instrument

#1 Model Varian UnityInova (500 MHz)

#2 Model Varian UnityInova (600 MHz)

#3 Model Bruker DRX (800 MHz)

Sample #1

Temperature 303 (±1.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	Cdc13 DNA-binding domain	[U-13C; U-15N]	0.7 ~ 1.1 mM
2	telomeric single-stranded DNA 11mer		0.8 ~ 1.2 mM
3	imidazole	[U-2H]	50 mM
4	sodium chloride		150 mM
5	sodium sulfate		100 mM
6	sodium azide		0.02 %
7	DTT	[U-2H]	2 mM

Sample #2

Temperature 303 (±1.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
8	Cdc13 DNA-binding domain	[U-15N]	1.5 mM
9	telomeric single-stranded DNA 11mer		1.7 mM
10	imidazole	[U-2H]	50 mM
11	sodium chloride		150 mM
12	sodium sulfate		100 mM
13	sodium azide		0.02 %
14	DTT	[U-2H]	2 mM

Sample #3

Temperature 303 (±1.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
15	Cdc13 DNA-binding domain		1.1 mM
16	telomeric single-stranded DNA 11mer		1.2 mM
17	imidazole	[U-2H]	50 mM
18	sodium chloride		150 mM
19	sodium sulfate		100 mM
20	sodium azide		0.02 %
21	DTT	[U-2H]	2 mM

11 3D HNCACB

Instrument

#1 Model Varian UnityInova (500 MHz)

#2 Model Varian UnityInova (600 MHz)

#3 Model Bruker DRX (800 MHz)

Sample #1

Temperature 303 (±1.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	Cdc13 DNA-binding domain	[U-13C; U-15N]	0.7 ~ 1.1 mM
2	telomeric single-stranded DNA 11mer		0.8 ~ 1.2 mM
3	imidazole	[U-2H]	50 mM
4	sodium chloride		150 mM
5	sodium sulfate		100 mM
6	sodium azide		0.02 %
7	DTT	[U-2H]	2 mM

Sample #2

Temperature 303 (±1.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
8	Cdc13 DNA-binding domain	[U-15N]	1.5 mM
9	telomeric single-stranded DNA 11mer		1.7 mM
10	imidazole	[U-2H]	50 mM
11	sodium chloride		150 mM
12	sodium sulfate		100 mM
13	sodium azide		0.02 %
14	DTT	[U-2H]	2 mM

Sample #3

Temperature 303 (±1.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
15	Cdc13 DNA-binding domain		1.1 mM
16	telomeric single-stranded DNA 11mer		1.2 mM
17	imidazole	[U-2H]	50 mM
18	sodium chloride		150 mM
19	sodium sulfate		100 mM
20	sodium azide		0.02 %
21	DTT	[U-2H]	2 mM

12 3D HCACO

Instrument

#1 Model Varian UnityInova (500 MHz)

#2 Model Varian UnityInova (600 MHz)

#3 Model Bruker DRX (800 MHz)

Sample #1

Temperature 303 (±1.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	Cdc13 DNA-binding domain	[U-13C; U-15N]	0.7 ~ 1.1 mM
2	telomeric single-stranded DNA 11mer		0.8 ~ 1.2 mM
3	imidazole	[U-2H]	50 mM
4	sodium chloride		150 mM
5	sodium sulfate		100 mM
6	sodium azide		0.02 %
7	DTT	[U-2H]	2 mM

Sample #2

Temperature 303 (±1.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
8	Cdc13 DNA-binding domain	[U-15N]	1.5 mM
9	telomeric single-stranded DNA 11mer		1.7 mM
10	imidazole	[U-2H]	50 mM
11	sodium chloride		150 mM
12	sodium sulfate		100 mM
13	sodium azide		0.02 %
14	DTT	[U-2H]	2 mM

Sample #3

Temperature 303 (±1.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
15	Cdc13 DNA-binding domain		1.1 mM
16	telomeric single-stranded DNA 11mer		1.2 mM
17	imidazole	[U-2H]	50 mM
18	sodium chloride		150 mM
19	sodium sulfate		100 mM
20	sodium azide		0.02 %
21	DTT	[U-2H]	2 mM

13 3D HNCA

Instrument

#1 Model Varian UnityInova (500 MHz)

#2 Model Varian UnityInova (600 MHz)

#3 Model Bruker DRX (800 MHz)

Sample #1

Temperature 303 (±1.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	Cdc13 DNA-binding domain	[U-13C; U-15N]	0.7 ~ 1.1 mM
2	telomeric single-stranded DNA 11mer		0.8 ~ 1.2 mM
3	imidazole	[U-2H]	50 mM
4	sodium chloride		150 mM
5	sodium sulfate		100 mM
6	sodium azide		0.02 %
7	DTT	[U-2H]	2 mM

Sample #2

Temperature 303 (±1.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
8	Cdc13 DNA-binding domain	[U-15N]	1.5 mM
9	telomeric single-stranded DNA 11mer		1.7 mM
10	imidazole	[U-2H]	50 mM
11	sodium chloride		150 mM
12	sodium sulfate		100 mM
13	sodium azide		0.02 %
14	DTT	[U-2H]	2 mM

Sample #3

Temperature 303 (±1.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
15	Cdc13 DNA-binding domain		1.1 mM
16	telomeric single-stranded DNA 11mer		1.2 mM
17	imidazole	[U-2H]	50 mM
18	sodium chloride		150 mM
19	sodium sulfate		100 mM
20	sodium azide		0.02 %
21	DTT	[U-2H]	2 mM

14 3D HNCO

Instrument

#1 Model Varian UnityInova (500 MHz)

#2 Model Varian UnityInova (600 MHz)

#3 Model Bruker DRX (800 MHz)

Sample #1

Temperature 303 (±1.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	Cdc13 DNA-binding domain	[U-13C; U-15N]	0.7 ~ 1.1 mM
2	telomeric single-stranded DNA 11mer		0.8 ~ 1.2 mM
3	imidazole	[U-2H]	50 mM
4	sodium chloride		150 mM
5	sodium sulfate		100 mM
6	sodium azide		0.02 %
7	DTT	[U-2H]	2 mM

Sample #2

Temperature 303 (±1.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
8	Cdc13 DNA-binding domain	[U-15N]	1.5 mM
9	telomeric single-stranded DNA 11mer		1.7 mM
10	imidazole	[U-2H]	50 mM
11	sodium chloride		150 mM
12	sodium sulfate		100 mM
13	sodium azide		0.02 %
14	DTT	[U-2H]	2 mM

Sample #3

Temperature 303 (±1.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
15	Cdc13 DNA-binding domain		1.1 mM
16	telomeric single-stranded DNA 11mer		1.2 mM
17	imidazole	[U-2H]	50 mM
18	sodium chloride		150 mM
19	sodium sulfate		100 mM
20	sodium azide		0.02 %
21	DTT	[U-2H]	2 mM

15 3D HN(CO)CA

Instrument

#1 Model Varian UnityInova (500 MHz)

#2 Model Varian UnityInova (600 MHz)

#3 Model Bruker DRX (800 MHz)

Sample #1

Temperature 303 (±1.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	Cdc13 DNA-binding domain	[U-13C; U-15N]	0.7 ~ 1.1 mM
2	telomeric single-stranded DNA 11mer		0.8 ~ 1.2 mM
3	imidazole	[U-2H]	50 mM
4	sodium chloride		150 mM
5	sodium sulfate		100 mM
6	sodium azide		0.02 %
7	DTT	[U-2H]	2 mM

Sample #2

Temperature 303 (±1.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
8	Cdc13 DNA-binding domain	[U-15N]	1.5 mM
9	telomeric single-stranded DNA 11mer		1.7 mM
10	imidazole	[U-2H]	50 mM
11	sodium chloride		150 mM
12	sodium sulfate		100 mM
13	sodium azide		0.02 %
14	DTT	[U-2H]	2 mM

Sample #3

Temperature 303 (±1.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
15	Cdc13 DNA-binding domain		1.1 mM
16	telomeric single-stranded DNA 11mer		1.2 mM
17	imidazole	[U-2H]	50 mM
18	sodium chloride		150 mM
19	sodium sulfate		100 mM
20	sodium azide		0.02 %
21	DTT	[U-2H]	2 mM

16 3D HN(CA)CO

Instrument

#1 Model Varian UnityInova (500 MHz)

#2 Model Varian UnityInova (600 MHz)

#3 Model Bruker DRX (800 MHz)

Sample #1

Temperature 303 (±1.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	Cdc13 DNA-binding domain	[U-13C; U-15N]	0.7 ~ 1.1 mM
2	telomeric single-stranded DNA 11mer		0.8 ~ 1.2 mM
3	imidazole	[U-2H]	50 mM
4	sodium chloride		150 mM
5	sodium sulfate		100 mM
6	sodium azide		0.02 %
7	DTT	[U-2H]	2 mM

Sample #2

Temperature 303 (±1.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
8	Cdc13 DNA-binding domain	[U-15N]	1.5 mM
9	telomeric single-stranded DNA 11mer		1.7 mM
10	imidazole	[U-2H]	50 mM
11	sodium chloride		150 mM
12	sodium sulfate		100 mM
13	sodium azide		0.02 %
14	DTT	[U-2H]	2 mM

Sample #3

Temperature 303 (±1.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
15	Cdc13 DNA-binding domain		1.1 mM
16	telomeric single-stranded DNA 11mer		1.2 mM
17	imidazole	[U-2H]	50 mM
18	sodium chloride		150 mM
19	sodium sulfate		100 mM
20	sodium azide		0.02 %
21	DTT	[U-2H]	2 mM

17 3D C(CO)NH

Instrument

#1 Model Varian UnityInova (500 MHz)

#2 Model Varian UnityInova (600 MHz)

#3 Model Bruker DRX (800 MHz)

Sample #1

Temperature 303 (±1.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	Cdc13 DNA-binding domain	[U-13C; U-15N]	0.7 ~ 1.1 mM
2	telomeric single-stranded DNA 11mer		0.8 ~ 1.2 mM
3	imidazole	[U-2H]	50 mM
4	sodium chloride		150 mM
5	sodium sulfate		100 mM
6	sodium azide		0.02 %
7	DTT	[U-2H]	2 mM

Sample #2

Temperature 303 (±1.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
8	Cdc13 DNA-binding domain	[U-15N]	1.5 mM
9	telomeric single-stranded DNA 11mer		1.7 mM
10	imidazole	[U-2H]	50 mM
11	sodium chloride		150 mM
12	sodium sulfate		100 mM
13	sodium azide		0.02 %
14	DTT	[U-2H]	2 mM

Sample #3

Temperature 303 (±1.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
15	Cdc13 DNA-binding domain		1.1 mM
16	telomeric single-stranded DNA 11mer		1.2 mM
17	imidazole	[U-2H]	50 mM
18	sodium chloride		150 mM
19	sodium sulfate		100 mM
20	sodium azide		0.02 %
21	DTT	[U-2H]	2 mM

18 3D HC(CO)NH

Instrument

#1 Model Varian UnityInova (500 MHz)

#2 Model Varian UnityInova (600 MHz)

#3 Model Bruker DRX (800 MHz)

Sample #1

Temperature 303 (±1.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	Cdc13 DNA-binding domain	[U-13C; U-15N]	0.7 ~ 1.1 mM
2	telomeric single-stranded DNA 11mer		0.8 ~ 1.2 mM
3	imidazole	[U-2H]	50 mM
4	sodium chloride		150 mM
5	sodium sulfate		100 mM
6	sodium azide		0.02 %
7	DTT	[U-2H]	2 mM

Sample #2

Temperature 303 (±1.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
8	Cdc13 DNA-binding domain	[U-15N]	1.5 mM
9	telomeric single-stranded DNA 11mer		1.7 mM
10	imidazole	[U-2H]	50 mM
11	sodium chloride		150 mM
12	sodium sulfate		100 mM
13	sodium azide		0.02 %
14	DTT	[U-2H]	2 mM

Sample #3

Temperature 303 (±1.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
15	Cdc13 DNA-binding domain		1.1 mM
16	telomeric single-stranded DNA 11mer		1.2 mM
17	imidazole	[U-2H]	50 mM
18	sodium chloride		150 mM
19	sodium sulfate		100 mM
20	sodium azide		0.02 %
21	DTT	[U-2H]	2 mM

19 4D 1H-15N-1H-13C NOESY

Instrument

#1 Model Varian UnityInova (500 MHz)

#2 Model Varian UnityInova (600 MHz)

#3 Model Bruker DRX (800 MHz)

Sample #1

Temperature 303 (±1.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	Cdc13 DNA-binding domain	[U-13C; U-15N]	0.7 ~ 1.1 mM
2	telomeric single-stranded DNA 11mer		0.8 ~ 1.2 mM
3	imidazole	[U-2H]	50 mM
4	sodium chloride		150 mM
5	sodium sulfate		100 mM
6	sodium azide		0.02 %
7	DTT	[U-2H]	2 mM

Sample #2

Temperature 303 (±1.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
8	Cdc13 DNA-binding domain	[U-15N]	1.5 mM
9	telomeric single-stranded DNA 11mer		1.7 mM
10	imidazole	[U-2H]	50 mM
11	sodium chloride		150 mM
12	sodium sulfate		100 mM
13	sodium azide		0.02 %
14	DTT	[U-2H]	2 mM

Sample #3

Temperature 303 (±1.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
15	Cdc13 DNA-binding domain		1.1 mM
16	telomeric single-stranded DNA 11mer		1.2 mM
17	imidazole	[U-2H]	50 mM
18	sodium chloride		150 mM
19	sodium sulfate		100 mM
20	sodium azide		0.02 %
21	DTT	[U-2H]	2 mM

20 4D 1H-13C-13C-1H NOESY

Instrument

#1 Model Varian UnityInova (500 MHz)

#2 Model Varian UnityInova (600 MHz)

#3 Model Bruker DRX (800 MHz)

Sample #1

Temperature 303 (±1.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	Cdc13 DNA-binding domain	[U-13C; U-15N]	0.7 ~ 1.1 mM
2	telomeric single-stranded DNA 11mer		0.8 ~ 1.2 mM
3	imidazole	[U-2H]	50 mM
4	sodium chloride		150 mM
5	sodium sulfate		100 mM
6	sodium azide		0.02 %
7	DTT	[U-2H]	2 mM

Sample #2

Temperature 303 (±1.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
8	Cdc13 DNA-binding domain	[U-15N]	1.5 mM
9	telomeric single-stranded DNA 11mer		1.7 mM
10	imidazole	[U-2H]	50 mM
11	sodium chloride		150 mM
12	sodium sulfate		100 mM
13	sodium azide		0.02 %
14	DTT	[U-2H]	2 mM

Sample #3

Temperature 303 (±1.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Concentration
15	Cdc13 DNA-binding domain		1.1 mM
16	telomeric single-stranded DNA 11mer		1.2 mM
17	imidazole	[U-2H]	50 mM
18	sodium chloride		150 mM
19	sodium sulfate		100 mM
20	sodium azide		0.02 %
21	DTT	[U-2H]	2 mM

nullKeywords cdc13, telomere-binding protein, single-stranded DNA binding protein

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BMRB: 5232

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Related entities 1. Cdc13 DNA-binding domain, : 1 : 1 : 2 entities Detail

Experiments performed 20 experiments Detail

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