BMRB: 5236

1H and 15N Chemical Shift Assignments for the tandem inactivation domain of Kv1.4 (RCK4(1-75))

Authors

Wissmann, R., Bildl, W., Oliver, D., Jonas, P., Bentrop, D., Fakler, B.

Assembly

RCK4

Entity

1. RCK4 (polymer, Thiol state: all free), 78 monomers, 7845.387 Da Detail

GSTMEVAMVS AESSGCNSHM PYGYAAQARA RERERLAHSR AAAAAAVAAA TAAVEGTGGS GGGPHHHHQT RGAYSSHD

Formula weight

7845.387 Da

Source organism

Rattus norvegicus

Exptl. method

NMR

Refine. method

simulated annealing, torsion angle dynamics

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete

Sequence coverage: 98.7 %, Completeness: 88.5 %, Completeness (bb): 95.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	88.5 % (407 of 460)	87.1 % (332 of 381)	94.9 % (75 of 79)
Backbone	95.8 % (230 of 240)	95.7 % (157 of 164)	96.1 % (73 of 76)
Sidechain	80.5 % (177 of 220)	80.6 % (175 of 217)	66.7 % (2 of 3)
Aromatic	23.1 % (6 of 26)	23.1 % (6 of 26)
Methyl	97.0 % (32 of 33)	97.0 % (32 of 33)

1. RCK4

GSTMEVAMVS AESSGCNSHM PYGYAAQARA RERERLAHSR AAAAAAVAAA TAAVEGTGGS GGGPHHHHQT RGAYSSHD

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Sample

Temperature 283 (±0.5) K, pH 4.4 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	RCK4	[U-98% 15N]		0.8 ~ 1.0 mM
2	sodium phosphate			50 mM
3	DTT			10 mM
4	pefablock SC			1 mM

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Protein Blocks Logo

Calculated from 25 models in PDB: 1KN7, Strand ID: A Detail

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Coupling constant

62 J values in 1 lists
Temperature 283 (±0.5) K, pH 4.4 (±0.2) Detail

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Temperature 283 (±0.5) K, pH 4.4 (±0.2)

List #1 J_values_set_1

Release date

2003-04-29

Citation

Solution structure and function of the "tandem inactivation domain" of the neuronal A-type potassium channel Kv1.4
Wissmann, R., Bildl, W., Oliver, D., Beyermann, M., Kalbitzer, H.R., Bentrop, D., Fakler, B.
J. Biol. Chem. (2003), 278, 16142-16150, PubMed 12590144 , DOI 10.1074/jbc.M210191200 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. RCK4, : 1 : 4 : 6 entities Detail

Interaction partners 1. RCK4, : 4 interactors Detail

Experiments performed 4 experiments Detail