BMRB: 5242

Solution Structure of the Prohormone Convertase 1 (PC1) Pro-Domain From Mus Musculus

Authors

Tangrea, M.A., Bryan, P.N., Orban, J.

Assembly

prohormone convertase 1 pro-domain

Entity

1. prohormone convertase 1 pro-domain (polymer, Thiol state: not present), 90 monomers, 10645.67 Da Detail

MHHHHHHKRQ FVNEWAAEIP GGQEAASAIA EELGYDLLGQ IGSLENHYLF KHKSHPRRSR RSALHITKRL SDDDRVTWAE QQYEKERSKR

Formula weight

10645.67 Da

Source organism

Mus musculus

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 91.1 %, Completeness: 68.6 %, Completeness (bb): 85.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	68.6 % (739 of 1077)	69.5 % (395 of 568)	65.0 % (268 of 412)	78.4 % (76 of 97)
Backbone	85.8 % (460 of 536)	88.5 % (162 of 183)	83.8 % (222 of 265)	86.4 % (76 of 88)
Sidechain	55.4 % (347 of 626)	60.5 % (233 of 385)	49.1 % (114 of 232)	0.0 % (0 of 9)
Aromatic	20.4 % (22 of 108)	40.7 % (22 of 54)	0.0 % (0 of 52)	0.0 % (0 of 2)
Methyl	94.4 % (68 of 72)	100.0 % (36 of 36)	88.9 % (32 of 36)

1. PC1 pro-domain

MHHHHHHKRQ FVNEWAAEIP GGQEAASAIA EELGYDLLGQ IGSLENHYLF KHKSHPRRSR RSALHITKRL SDDDRVTWAE QQYEKERSKR

Show sample condition

Sample

Temperature 298 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	PC1 pro-domain	[U-13C; U-15N]		2.0 mM

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LACS Plot; CA

Referencing offset: -0.64 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.64 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.02 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.16 ppm, Outliers: 4 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1KN6, Strand ID: A Detail

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Release date

2002-09-09

Citation

Solution structure of the pro-hormone convertase 1 pro-domain from Mus musculus
Tangrea, M.A., Bryan, P.N., Sari, N., Orban, J.
J. Mol. Biol. (2002), 320, 801-812, PubMed 12095256 , DOI: , Abstract

Related entities 1. prohormone convertase 1 pro-domain, : 1 : 3 : 52 entities Detail

Interaction partners 1. prohormone convertase 1 pro-domain, : 2 interactors Detail

Experiments performed 6 experiments Detail