BMRB: 5243

Solution Structure of the 17mer TF1 Binding Site

Authors

Liu, W., Vu, H., Kearns, D.R.

Assembly

5'-D(*CP*AP*CP*TP*AP*CP*TP*CP*TP*TP*TP*GP*TP*AP*GP*TP*G)-3'

Entity

1. 5'-D(*CP*AP*CP*TP*AP*CP*TP*CP*TP*TP*TP*GP*TP*AP*GP*TP*G)-3' (polymer, Thiol state: not present), 34 monomers, 10520.72 Da Detail

CACTACTCTT TGTAGTGCAC TACAAAGAGT AGTG

Formula weight

10520.72 Da

Source organism

Bacillus subtilis

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 97.9 %, Completeness (bb): 99.2 % Detail

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Polymer type: polydeoxyribonucleotide

	Total	1H
All	97.9 % (323 of 330)	97.9 % (323 of 330)
Suger, PO4	99.2 % (236 of 238)	99.2 % (236 of 238)
Nucleobase	94.6 % (87 of 92)	94.6 % (87 of 92)
Aromatic	98.5 % (67 of 68)	98.5 % (67 of 68)
Methyl	100.0 % (10 of 10)	100.0 % (10 of 10)

1. 17mer TF1 Binding Site

CACTACTCTT TGTAGTGCAC TACAAAGAGT AGTG

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	17mer TF1 Binding Site			2 mM
2	phosphate buffer			100 mM
3	H2O			90 %
4	D2O			10 %

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Release date

2002-06-12

Citation

1H NMR studies of a 17-mer DNA duplex
Liu, W., Vu, H.M., Kearns, D.R.
Biochim. Biophys. Acta (2002), 1574, 93-99, PubMed 11955617 , DOI: , Abstract

Experiments performed 3 experiments Detail