BMRB: 5255

Ancestral beta/gamma-crystallin precursor structure in a yeast killer toxin

Authors

Antuch, W., Guntert, P., Wuthrich, K.

Assembly

WmKT

Entity

1. WmKT (polymer, Thiol state: all disulfide bound), 88 monomers, 9527.675 Da Detail

GDGYLIMCKN CDPNTGSCDW KQNWNTCVGI GANVHWMVTG GSTDGKQGCA TIWEGSGCVG RSTTMCCPAN TCCNINTGFY IRSYRRVE

Formula weight

9527.675 Da

Entity Connection

disulfide 5 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	0:CYS8:SG	1:CYS18:SG
2	disulfide	sing	0:CYS11:SG	1:CYS72:SG
3	disulfide	sing	0:CYS27:SG	1:CYS58:SG
4	disulfide	sing	0:CYS49:SG	1:CYS66:SG
5	disulfide	sing	0:CYS67:SG	1:CYS73:SG

Source organism

Saccharomyces cerevisiae

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 92.5 %, Completeness (bb): 98.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	92.5 % (447 of 483)	92.5 % (447 of 483)
Backbone	98.4 % (184 of 187)	98.4 % (184 of 187)
Sidechain	88.9 % (263 of 296)	88.9 % (263 of 296)
Aromatic	79.1 % (34 of 43)	79.1 % (34 of 43)
Methyl	88.2 % (30 of 34)	88.2 % (30 of 34)

1. yeast killer toxin

GDGYLIMCKN CDPNTGSCDW KQNWNTCVGI GANVHWMVTG GSTDGKQGCA TIWEGSGCVG RSTTMCCPAN TCCNINTGFY IRSYRRVE

Show sample condition

Sample

Temperature 309 (±1) K, pH 5.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	yeast killer toxin			3.0 mM

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Protein Blocks Logo

Calculated from 20 models in PDB: 1WKT, Strand ID: A Detail

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Release date

2002-02-07

Citation

Ancestral beta gamma-crystallin precursor structure in a yeast killer toxin
Antuch, W., Guntert, P., Wuthrich, K.
Nat. Struct. Biol. (1996), 3, 662-665, PubMed 8756320 , DOI: , Abstract

Related entities 1. WmKT, : 1 : 1 : 8 entities Detail