BMRB: 5261

Nuclease A Inhibitor (NuiA) Assignments

Authors

Kirby, T.W., Mueller, G.A., DeRose, E.F., Lebetkin, M.S., Meiss, G., Pingoud, A., London, R.E.

Assembly

Nuclease A Inhibitor

Entity

1. Nuclease A Inhibitor (polymer, Thiol state: not present), 143 monomers, 16075.73 Da Detail

MHHHHHHGST KTNSEILEQL KQASDGLLFM SESEYPFEVF LWEGSAPPVT HEIVLQQTGH GQDAPFKVVD IDSFFSRATT PQDWYEDEEN AVVAKFQKLL EVIKSNLKNP QVYRLGEVEL DVYVIGETPA GNLAGISTKV VET

Formula weight

16075.73 Da

Source organism

Nostoc sp. PCC 7120 = FACHB-418

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.7 %, Completeness: 89.7 %, Completeness (bb): 90.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	89.7 % (1496 of 1667)	90.0 % (773 of 859)	89.0 % (585 of 657)	91.4 % (138 of 151)
Backbone	90.9 % (767 of 844)	91.3 % (263 of 288)	90.7 % (381 of 420)	90.4 % (123 of 136)
Sidechain	89.0 % (852 of 957)	89.3 % (510 of 571)	88.1 % (327 of 371)	100.0 % (15 of 15)
Aromatic	67.7 % (107 of 158)	67.1 % (53 of 79)	67.5 % (52 of 77)	100.0 % (2 of 2)
Methyl	96.3 % (156 of 162)	96.3 % (78 of 81)	96.3 % (78 of 81)

1. Nuclease A Inhibitor

MHHHHHHGST KTNSEILEQL KQASDGLLFM SESEYPFEVF LWEGSAPPVT HEIVLQQTGH GQDAPFKVVD IDSFFSRATT PQDWYEDEEN AVVAKFQKLL EVIKSNLKNP QVYRLGEVEL DVYVIGETPA GNLAGISTKV VET

Show sample condition

Sample

Temperature 303 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Nuclease A Inhibitor	[U-13C; U-15N]		3.3 mM
2	Tris	[U-2H]		90 mM

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LACS Plot; CA

Referencing offset: 0.06 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CB

Referencing offset: 0.06 ppm, Outliers: 2 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; HA

Referencing offset: -0.07 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CO

Referencing offset: 0.04 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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Protein Blocks Logo

Calculated from 7 models in PDB: 1KTU, Strand ID: A Detail

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Release date

2002-09-09

Citation

The nuclease A inhibitor represents a new variation of the rare PR-1 fold
Kirby, T.W., Mueller, G.A., DeRose, E.F., Lebetkin, M.S., Meiss, G., Pingoud, A., London, R.E.
J. Mol. Biol. (2002), 320, 771-782, PubMed 12095254 , DOI 10.1016/s0022-2836(02)00460-6 , Abstract

Related entities 1. Nuclease A Inhibitor, : 1 : 1 : 1 entities Detail

Experiments performed 1 experiments Detail