BMRB: 5278

PEMV-1 P1-P2 Frameshifting Pseudoknot Regularized Average Structure

Authors

Nixon, P.L., Giedroc, D.P.

Assembly

Pea Enation Mosaic Virus P1-P2 frameshifting pseudoknot

Entity

1. Pea Enation Mosaic Virus P1-P2 frameshifting pseudoknot (polymer, Thiol state: not present), 33 monomers, 10703.42 Da Detail

GAAUUCCGGU XGACUCCGGA GAAACAAAGU CAA

Formula weight

10703.42 Da

Source organism

Pea enation mosaic virus 1

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.9 %, Completeness: 40.5 %, Completeness (bb): 30.4 % Detail

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Polymer type: polyribonucleotide

	Total	1H	13C	15N
All	40.5 % (204 of 504)	56.0 % (154 of 275)	19.4 % (42 of 216)	61.5 % (8 of 13)
Suger, PO4	30.4 % (107 of 352)	42.2 % (81 of 192)	16.2 % (26 of 160)
Nucleobase	63.8 % (97 of 152)	88.0 % (73 of 83)	28.6 % (16 of 56)	61.5 % (8 of 13)
Aromatic	61.6 % (85 of 138)	88.4 % (61 of 69)	28.6 % (16 of 56)	61.5 % (8 of 13)

1. Pea Enation Mosaic Virus Pseudoknot

GAAUUCCGGU XGACUCCGGA GAAACAAAGU CAA

Show sample condition

Sample

Pressure 1 atm, Temperature 283 (±1) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Pea Enation Mosaic Virus Pseudoknot			2 mM
2	KPO4			10 mM
3	KCl			100 mM
4	MgCl2			5 mM

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Release date

2003-02-19

Citation

Solution structure of a luteoviral P1-P2 frameshifting mRNA pseudoknot
Nixon, P.L., Rangan, A., Kim, Y., Rich, A., Hoffman, D.W., Hennig, M., Giedroc, D.P.
J. Mol. Biol. (2002), 322, 621-633, PubMed 12225754 , DOI: , Abstract

Related entities 1. Pea Enation Mosaic Virus P1-P2 frameshifting pseudoknot, : 1 : 1 : 2 entities Detail

Experiments performed 1 experiments Detail