BMRBj - BMREntryMaster

Found 1 Document (1.586 seconds)

BMRB: 5282

1PQT

Refinement of d(GCGAAGC) Hairpin Structure Using One- and Two-Bonds Residual Dipolar Couplings

Authors

Padrta, P., Stefl, R., Zidek, L., Sklenar, V.

Assembly

5'-D(*GP*CP*GP*AP*AP*GP*C)-3'

Entity

1. 5'-D(*GP*CP*GP*AP*AP*GP*C)-3' (polymer, Thiol state: not present), 7 monomers, 2210.409 Da Detail

GCGAAGC

Formula weight

2210.409 Da

Source organism

Simplexvirus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 97.3 %, Completeness (bb): 96.4 % Detail

Polymer type: polydeoxyribonucleotide

	Total	¹H	³¹P
All	97.3 % (72 of 74)	98.5 % (66 of 67)	85.7 % (6 of 7)
Suger, PO4	96.4 % (54 of 56)	98.0 % (48 of 49)	85.7 % (6 of 7)
Nucleobase	100.0 % (18 of 18)	100.0 % (18 of 18)
Aromatic	100.0 % (14 of 14)	100.0 % (14 of 14)

1. GCGAAGC hairpin

GCGAAGC

Show sample condition

Sample #1

Temperature 303 K, pH 6.7

#	Name	Concentration
1	GCGAAGC hairpin	6 mM
2	Na phosphate buffer	10 mM
3	D2O	100 %

Sample #2

Temperature 303 K, pH 6.7

#	Name	Concentration
4	GCGAAGC hairpin	6 mM
5	Na phosphate buffer	10 mM
6	H2O	90 %
7	D2O	10 %

Sample #3

Temperature 303 K, pH 6.7

#	Name	Isotope labeling	Concentration
8	GCGAAGC hairpin	[U-13C; U-15N]	0.5 mM
9	Na phosphate buffer		10 mM
10	H2O		90 %
11	D2O		10 %

Release date

2003-02-19

Citation

Refinement of d(GCGAAGC) hairpin structure using one- and two-bond residual dipolar couplings
Padrta, P., Stefl, R., Kralik, L., Zidek, L., Sklenar, V.
J. Biomol. NMR (2002), 24, 1-14, PubMed 12449414 , DOI: , Abstract

Experiments performed 5 experiments Detail

1 2D NOESY

Instrument

Model Bruker AVANCE-DRX (500 MHz)

Sample #1

Temperature 303 K, pH 6.7

#	Name	Concentration
1	GCGAAGC hairpin	6 mM
2	Na phosphate buffer	10 mM
3	D2O	100 %

Sample #2

Temperature 303 K, pH 6.7

#	Name	Concentration
4	GCGAAGC hairpin	6 mM
5	Na phosphate buffer	10 mM
6	H2O	90 %
7	D2O	10 %

Sample #3

Temperature 303 K, pH 6.7

#	Name	Isotope labeling	Concentration
8	GCGAAGC hairpin	[U-13C; U-15N]	0.5 mM
9	Na phosphate buffer		10 mM
10	H2O		90 %
11	D2O		10 %

2 E-COSY

Instrument

Model Bruker AVANCE-DRX (500 MHz)

Sample #1

Temperature 303 K, pH 6.7

#	Name	Concentration
1	GCGAAGC hairpin	6 mM
2	Na phosphate buffer	10 mM
3	D2O	100 %

Sample #2

Temperature 303 K, pH 6.7

#	Name	Concentration
4	GCGAAGC hairpin	6 mM
5	Na phosphate buffer	10 mM
6	H2O	90 %
7	D2O	10 %

Sample #3

Temperature 303 K, pH 6.7

#	Name	Isotope labeling	Concentration
8	GCGAAGC hairpin	[U-13C; U-15N]	0.5 mM
9	Na phosphate buffer		10 mM
10	H2O		90 %
11	D2O		10 %

3 DQF-COSY

Instrument

Model Bruker AVANCE-DRX (500 MHz)

Sample #1

Temperature 303 K, pH 6.7

#	Name	Concentration
1	GCGAAGC hairpin	6 mM
2	Na phosphate buffer	10 mM
3	D2O	100 %

Sample #2

Temperature 303 K, pH 6.7

#	Name	Concentration
4	GCGAAGC hairpin	6 mM
5	Na phosphate buffer	10 mM
6	H2O	90 %
7	D2O	10 %

Sample #3

Temperature 303 K, pH 6.7

#	Name	Isotope labeling	Concentration
8	GCGAAGC hairpin	[U-13C; U-15N]	0.5 mM
9	Na phosphate buffer		10 mM
10	H2O		90 %
11	D2O		10 %

4 COUPLED HSQC

Instrument

Model Bruker AVANCE-DRX (500 MHz)

Sample #1

Temperature 303 K, pH 6.7

#	Name	Concentration
1	GCGAAGC hairpin	6 mM
2	Na phosphate buffer	10 mM
3	D2O	100 %

Sample #2

Temperature 303 K, pH 6.7

#	Name	Concentration
4	GCGAAGC hairpin	6 mM
5	Na phosphate buffer	10 mM
6	H2O	90 %
7	D2O	10 %

Sample #3

Temperature 303 K, pH 6.7

#	Name	Isotope labeling	Concentration
8	GCGAAGC hairpin	[U-13C; U-15N]	0.5 mM
9	Na phosphate buffer		10 mM
10	H2O		90 %
11	D2O		10 %

5 S3E E-COSY

Instrument

Model Bruker AVANCE-DRX (500 MHz)

Sample #1

Temperature 303 K, pH 6.7

#	Name	Concentration
1	GCGAAGC hairpin	6 mM
2	Na phosphate buffer	10 mM
3	D2O	100 %

Sample #2

Temperature 303 K, pH 6.7

#	Name	Concentration
4	GCGAAGC hairpin	6 mM
5	Na phosphate buffer	10 mM
6	H2O	90 %
7	D2O	10 %

Sample #3

Temperature 303 K, pH 6.7

#	Name	Isotope labeling	Concentration
8	GCGAAGC hairpin	[U-13C; U-15N]	0.5 mM
9	Na phosphate buffer		10 mM
10	H2O		90 %
11	D2O		10 %

nullKeywords DNA, GA mismatch, hairpin

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Found 1 Document (1.586 seconds)

BMRB: 5282

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Experiments performed 5 experiments Detail

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