BMRB: 5283

Proton Chemical Shift Assignments for Vpr(34-51) Peptide

Authors

Engler, A., Stangler, T., Willbold, D.

Assembly

HIV-1 Vpr (34-51)

Entity

1. HIV-1 Vpr (34-51) (polymer, Thiol state: not present), 20 monomers, 2268.553 Da Detail

XFPRIWLHNL GQHIYETYGX

Formula weight

2268.553 Da

Source organism

Human immunodeficiency virus 1

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 90.0 %, Completeness: 99.2 %, Completeness (bb): 100.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	99.2 % (120 of 121)	99.2 % (120 of 121)
Backbone	100.0 % (37 of 37)	100.0 % (37 of 37)
Sidechain	98.8 % (83 of 84)	98.8 % (83 of 84)
Aromatic	95.7 % (22 of 23)	95.7 % (22 of 23)
Methyl	100.0 % (9 of 9)	100.0 % (9 of 9)

1. Vpr(34-51)

XFPRIWLHNL GQHIYETYGX

Show sample condition

Sample

Temperature 298 (±1) K, pH 4.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Vpr(34-51)			2.0 mM
2	DPC-d38			100 mM
3	D2O			10 % v/v
4	H2O			90 % v/v

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Chem. Shift Complete2

Sequence coverage: 90.0 %, Completeness: 98.3 %, Completeness (bb): 100.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	98.3 % (238 of 242)	98.3 % (238 of 242)
Backbone	100.0 % (74 of 74)	100.0 % (74 of 74)
Sidechain	97.6 % (164 of 168)	97.6 % (164 of 168)
Aromatic	91.3 % (42 of 46)	91.3 % (42 of 46)
Methyl	100.0 % (18 of 18)	100.0 % (18 of 18)

1. Vpr(34-51)

XFPRIWLHNL GQHIYETYGX

Show sample condition

Sample

Temperature 298 (±1) K

#	Name	Isotope labeling	Type	Concentration
5	Vpr(34-51)			2 mM
6	Trifluoroethanol-d2			50 % v/v
7	H2O			50 % v/v

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Chem. Shift Complete3

Sequence coverage: 90.0 %, Completeness: 98.6 %, Completeness (bb): 100.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	98.6 % (358 of 363)	98.6 % (358 of 363)
Backbone	100.0 % (111 of 111)	100.0 % (111 of 111)
Sidechain	98.0 % (247 of 252)	98.0 % (247 of 252)
Aromatic	92.8 % (64 of 69)	92.8 % (64 of 69)
Methyl	100.0 % (27 of 27)	100.0 % (27 of 27)

1. Vpr(34-51)

XFPRIWLHNL GQHIYETYGX

Show sample condition

Sample

Temperature 298 (±1) K

#	Name	Isotope labeling	Type	Concentration
8	Vpr(34-51)			2 mM
9	Methanol-d2			50 % v/v
10	Chloroform-d			50 % v/v

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Protein Blocks Logo

Calculated from 20 models in PDB: 1KZV, Strand ID: A Detail

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Release date

2015-08-04

Citation

Structure of human immunodeficiency virus type 1 Vpr(34-51) peptide in micelle containing aqueous solution
Engler, A., Stangler, T., Willbold, D.
Eur. J. Biochem. (2002), 269, 3264-3269, PubMed 12084067 , DOI 10.2210/pdb1kzt/pdb , Abstract

Related entities 1. HIV-1 Vpr (34-51), : 1 : 10 : 35 entities Detail

Interaction partners 1. HIV-1 Vpr (34-51), : 11 interactors Detail

Experiments performed 3 experiments Detail