BMRBj - BMREntryMaster

	Total	¹H	¹⁵N
All	98.8 % (955 of 967)	98.8 % (811 of 821)	98.6 % (144 of 146)
Backbone	98.5 % (397 of 403)	98.5 % (268 of 272)	98.5 % (129 of 131)
Sidechain	98.9 % (558 of 564)	98.9 % (543 of 549)	100.0 % (15 of 15)
Aromatic	100.0 % (72 of 72)	100.0 % (70 of 70)	100.0 % (2 of 2)
Methyl	95.8 % (69 of 72)	95.8 % (69 of 72)

1. intestinal fatty acid binding protein

AFDSTWKVDR SENYDKFMEK MGVNIVKRKL AAHDNLKLTI TQEGNKFTVK ESSAFRNIEV VFELGVTFNY NLADGTELRG TWSLEGNKLI GKFKRTDNGN ELNTVREIIG DELVQTYVYE GVEAKRIFKK D

Show sample condition

Sample #1

Temperature 310 (±0.1) K, pH 6.5 (±0.1), Details Contains a mixture of endogenous fatty acids that are bound to the protein.

#	Name	Isotope labeling	Type	Concentration
1	intestinal fatty acid binding protein			3.0 ~ 4.0 mM

Sample #2

Temperature 310 (±0.1) K, pH 6.5 (±0.1), Details Contains a mixture of endogenous fatty acids that are bound to the protein.

#	Name	Isotope labeling	Type	Concentration
2	intestinal fatty acid binding protein	[U-15N]		3.0 ~ 4.0 mM

Protein Blocks Logo

Calculated from 20 models in PDB: 1KZW, Strand ID: A Detail

Release date

2003-06-24

Citation

Solution structure of human intestinal fatty acid binding protein with a naturally-occurring single amino acid substitution (A54T) that is associated with altered lipid metabolism
Zhang, F., Luecke, C., Baier, L.J., Sacchettini, J.C., Hamilton, J.A.
Biochemistry (2003), 42, 7339-7349, PubMed , DOI:

Related entities 1. A54 I-FABP, : 1 : 6 : 2 : 249 entities Detail

Interaction partners 1. A54 I-FABP, : 3 interactors Detail

More details for 3 interactors identified by 3 citations

Experiments performed 6 experiments Detail

1 1H-1H TOCSY

Instrument

Model Bruker DMX (500 MHz)

Sample #1

Temperature 310 (±0.1) K, pH 6.5 (±0.1), Details Contains a mixture of endogenous fatty acids that are bound to the protein.

#	Name	Isotope labeling	Type	Concentration
1	intestinal fatty acid binding protein			3.0 ~ 4.0 mM

Sample #2

Temperature 310 (±0.1) K, pH 6.5 (±0.1), Details Contains a mixture of endogenous fatty acids that are bound to the protein.

#	Name	Isotope labeling	Type	Concentration
2	intestinal fatty acid binding protein	[U-15N]		3.0 ~ 4.0 mM

2 1H-1H NOESY

Instrument

Model Bruker DMX (500 MHz)

Sample #1

Temperature 310 (±0.1) K, pH 6.5 (±0.1), Details Contains a mixture of endogenous fatty acids that are bound to the protein.

#	Name	Isotope labeling	Type	Concentration
1	intestinal fatty acid binding protein			3.0 ~ 4.0 mM

Sample #2

Temperature 310 (±0.1) K, pH 6.5 (±0.1), Details Contains a mixture of endogenous fatty acids that are bound to the protein.

#	Name	Isotope labeling	Type	Concentration
2	intestinal fatty acid binding protein	[U-15N]		3.0 ~ 4.0 mM

3 1H-15N HSQC

Instrument

Model Bruker DMX (500 MHz)

Sample #1

Temperature 310 (±0.1) K, pH 6.5 (±0.1), Details Contains a mixture of endogenous fatty acids that are bound to the protein.

#	Name	Isotope labeling	Type	Concentration
1	intestinal fatty acid binding protein			3.0 ~ 4.0 mM

Sample #2

Temperature 310 (±0.1) K, pH 6.5 (±0.1), Details Contains a mixture of endogenous fatty acids that are bound to the protein.

#	Name	Isotope labeling	Type	Concentration
2	intestinal fatty acid binding protein	[U-15N]		3.0 ~ 4.0 mM

4 1H-15N HTQC

Instrument

Model Bruker DMX (500 MHz)

Sample #1

Temperature 310 (±0.1) K, pH 6.5 (±0.1), Details Contains a mixture of endogenous fatty acids that are bound to the protein.

#	Name	Isotope labeling	Type	Concentration
1	intestinal fatty acid binding protein			3.0 ~ 4.0 mM

Sample #2

Temperature 310 (±0.1) K, pH 6.5 (±0.1), Details Contains a mixture of endogenous fatty acids that are bound to the protein.

#	Name	Isotope labeling	Type	Concentration
2	intestinal fatty acid binding protein	[U-15N]		3.0 ~ 4.0 mM

5 3D 1H-15N TOCSY-HMQC

Instrument

Model Bruker DMX (500 MHz)

Sample #1

Temperature 310 (±0.1) K, pH 6.5 (±0.1), Details Contains a mixture of endogenous fatty acids that are bound to the protein.

#	Name	Isotope labeling	Type	Concentration
1	intestinal fatty acid binding protein			3.0 ~ 4.0 mM

Sample #2

Temperature 310 (±0.1) K, pH 6.5 (±0.1), Details Contains a mixture of endogenous fatty acids that are bound to the protein.

#	Name	Isotope labeling	Type	Concentration
2	intestinal fatty acid binding protein	[U-15N]		3.0 ~ 4.0 mM

6 3D 1H-15N NOESY-HMQC

Instrument

Model Bruker DMX (500 MHz)

Sample #1

Temperature 310 (±0.1) K, pH 6.5 (±0.1), Details Contains a mixture of endogenous fatty acids that are bound to the protein.

#	Name	Isotope labeling	Type	Concentration
1	intestinal fatty acid binding protein			3.0 ~ 4.0 mM

Sample #2

Temperature 310 (±0.1) K, pH 6.5 (±0.1), Details Contains a mixture of endogenous fatty acids that are bound to the protein.

#	Name	Isotope labeling	Type	Concentration
2	intestinal fatty acid binding protein	[U-15N]		3.0 ~ 4.0 mM

nullKeywords fatty acid binding, type 2 diabetes, single base polymorphism, holo-form

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BMRB: 5284

Sample #1

Sample #2

Related entities 1. A54 I-FABP, : 1 : 6 : 2 : 249 entities Detail

Interaction partners 1. A54 I-FABP, : 3 interactors Detail

Experiments performed 6 experiments Detail

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