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1H and 15N Chemical Shift Assignments for Human T54 I-FABP
Authors
Zhang, F., Luecke, C., Baier, L.J., Sacchettini, J.C., Hamilton, J.A.
Assembly
T54 I-FABP
Entity
1. T54 I-FABP (polymer, Thiol state: not present), 131 monomers, 15105.88 Da Detail

AFDSTWKVDR SENYDKFMEK MGVNIVKRKL AAHDNLKLTI TQEGNKFTVK ESSTFRNIEV VFELGVTFNY NLADGTELRG TWSLEGNKLI GKFKRTDNGN ELNTVREIIG DELVQTYVYE GVEAKRIFKK D


Formula weight
15105.88 Da
Source organism
Homo sapiens
Exptl. method
NMR
Refine. method
simulated annealing combined with torsion angle dynamics and followed by energy minimization
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 99.2 %, Completeness: 98.2 %, Completeness (bb): 98.5 % Detail

Polymer type: polypeptide(L)

Total1H15N
All98.2 % (951 of 968)98.5 % (810 of 822)96.6 % (141 of 146)
Backbone98.5 % (397 of 403)98.9 % (269 of 272)97.7 % (128 of 131)
Sidechain98.1 % (554 of 565)98.4 % (541 of 550)86.7 % (13 of 15)
Aromatic100.0 % (72 of 72)100.0 % (70 of 70)100.0 % (2 of 2)
Methyl98.6 % (71 of 72)98.6 % (71 of 72)

1. intestinal fatty acid binding protein

AFDSTWKVDR SENYDKFMEK MGVNIVKRKL AAHDNLKLTI TQEGNKFTVK ESSTFRNIEV VFELGVTFNY NLADGTELRG TWSLEGNKLI GKFKRTDNGN ELNTVREIIG DELVQTYVYE GVEAKRIFKK D

Sample

Temperature 310 (±0.1) K, pH 6.5 (±0.1), Details Contains a mixture of endogenous fatty acids that are bound to the protein.


#NameIsotope labelingTypeConcentration
1intestinal fatty acid binding protein3 mM

Protein Blocks Logo
Calculated from 20 models in PDB: 1KZX, Strand ID: A Detail


Release date
2003-06-24
Citation
Solution structure of human intestinal fatty acid binding protein with a naturally-occurring single amino acid substitution (A54T) that is associated with altered lipid metabolism
Zhang, F., Luecke, C., Baier, L.J., Sacchettini, J.C., Hamilton, J.A.
Biochemistry (2003), 42, 7339-7347, PubMed 12809489 , DOI 10.1021/bi0273617 ,
Related entities 1. T54 I-FABP, : 1 : 8 : 249 entities Detail
Interaction partners 1. T54 I-FABP, : 3 interactors Detail
Experiments performed 6 experiments Detail
nullKeywords fatty acid binding, type 2 diabetes, single base polymorphism, holo-form