BMRBj - BMREntryMaster

	Total	¹H	¹⁵N
All	98.2 % (951 of 968)	98.5 % (810 of 822)	96.6 % (141 of 146)
Backbone	98.5 % (397 of 403)	98.9 % (269 of 272)	97.7 % (128 of 131)
Sidechain	98.1 % (554 of 565)	98.4 % (541 of 550)	86.7 % (13 of 15)
Aromatic	100.0 % (72 of 72)	100.0 % (70 of 70)	100.0 % (2 of 2)
Methyl	98.6 % (71 of 72)	98.6 % (71 of 72)

1. intestinal fatty acid binding protein

AFDSTWKVDR SENYDKFMEK MGVNIVKRKL AAHDNLKLTI TQEGNKFTVK ESSTFRNIEV VFELGVTFNY NLADGTELRG TWSLEGNKLI GKFKRTDNGN ELNTVREIIG DELVQTYVYE GVEAKRIFKK D

Show sample condition

Sample

Temperature 310 (±0.1) K, pH 6.5 (±0.1), Details Contains a mixture of endogenous fatty acids that are bound to the protein.

#	Name	Isotope labeling	Type	Concentration
1	intestinal fatty acid binding protein			3 mM

Protein Blocks Logo

Calculated from 20 models in PDB: 1KZX, Strand ID: A Detail

Release date

2003-06-24

Citation

Solution structure of human intestinal fatty acid binding protein with a naturally-occurring single amino acid substitution (A54T) that is associated with altered lipid metabolism
Zhang, F., Luecke, C., Baier, L.J., Sacchettini, J.C., Hamilton, J.A.
Biochemistry (2003), 42, 7339-7347, PubMed 12809489 , DOI 10.1021/bi0273617 , Abstract

Related entities 1. T54 I-FABP, : 1 : 8 : 249 entities Detail

Interaction partners 1. T54 I-FABP, : 3 interactors Detail

More details for 3 interactors identified by 3 citations

Experiments performed 6 experiments Detail

1 1H-1H TOCSY

Instrument

Model Bruker DMX (500 MHz)

Sample #1

Temperature 310 (±0.1) K, pH 6.5 (±0.1), Details Contains a mixture of endogenous fatty acids that are bound to the protein.

#	Name	Isotope labeling	Type	Concentration
1	intestinal fatty acid binding protein			3 mM

Sample #2

Temperature 310 (±0.1) K, pH 6.5 (±0.1), Details Contains a mixture of endogenous fatty acids that are bound to the protein.

#	Name	Isotope labeling	Type	Concentration
2	intestinal fatty acid binding protein	[U-15N]		3 mM

2 1H-1H NOESY

Instrument

Model Bruker DMX (500 MHz)

Sample #1

Temperature 310 (±0.1) K, pH 6.5 (±0.1), Details Contains a mixture of endogenous fatty acids that are bound to the protein.

#	Name	Isotope labeling	Type	Concentration
1	intestinal fatty acid binding protein			3 mM

Sample #2

Temperature 310 (±0.1) K, pH 6.5 (±0.1), Details Contains a mixture of endogenous fatty acids that are bound to the protein.

#	Name	Isotope labeling	Type	Concentration
2	intestinal fatty acid binding protein	[U-15N]		3 mM

3 1H-15N HSQC

Instrument

Model Bruker DMX (500 MHz)

Sample #1

Temperature 310 (±0.1) K, pH 6.5 (±0.1), Details Contains a mixture of endogenous fatty acids that are bound to the protein.

#	Name	Isotope labeling	Type	Concentration
1	intestinal fatty acid binding protein			3 mM

Sample #2

Temperature 310 (±0.1) K, pH 6.5 (±0.1), Details Contains a mixture of endogenous fatty acids that are bound to the protein.

#	Name	Isotope labeling	Type	Concentration
2	intestinal fatty acid binding protein	[U-15N]		3 mM

4 1H-15N HTQC

Instrument

Model Bruker DMX (500 MHz)

Sample #1

Temperature 310 (±0.1) K, pH 6.5 (±0.1), Details Contains a mixture of endogenous fatty acids that are bound to the protein.

#	Name	Isotope labeling	Type	Concentration
1	intestinal fatty acid binding protein			3 mM

Sample #2

Temperature 310 (±0.1) K, pH 6.5 (±0.1), Details Contains a mixture of endogenous fatty acids that are bound to the protein.

#	Name	Isotope labeling	Type	Concentration
2	intestinal fatty acid binding protein	[U-15N]		3 mM

5 3D 1H-15N TOCSY-HMQC

Instrument

Model Bruker DMX (500 MHz)

Sample #1

Temperature 310 (±0.1) K, pH 6.5 (±0.1), Details Contains a mixture of endogenous fatty acids that are bound to the protein.

#	Name	Isotope labeling	Type	Concentration
1	intestinal fatty acid binding protein			3 mM

Sample #2

Temperature 310 (±0.1) K, pH 6.5 (±0.1), Details Contains a mixture of endogenous fatty acids that are bound to the protein.

#	Name	Isotope labeling	Type	Concentration
2	intestinal fatty acid binding protein	[U-15N]		3 mM

6 3D 1H-15N NOESY-HMQC

Instrument

Model Bruker DMX (500 MHz)

Sample #1

Temperature 310 (±0.1) K, pH 6.5 (±0.1), Details Contains a mixture of endogenous fatty acids that are bound to the protein.

#	Name	Isotope labeling	Type	Concentration
1	intestinal fatty acid binding protein			3 mM

Sample #2

Temperature 310 (±0.1) K, pH 6.5 (±0.1), Details Contains a mixture of endogenous fatty acids that are bound to the protein.

#	Name	Isotope labeling	Type	Concentration
2	intestinal fatty acid binding protein	[U-15N]		3 mM

nullKeywords fatty acid binding, type 2 diabetes, single base polymorphism, holo-form

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BMRB: 5285

Sample

Related entities 1. T54 I-FABP, : 1 : 8 : 249 entities Detail

Interaction partners 1. T54 I-FABP, : 3 interactors Detail

Experiments performed 6 experiments Detail

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