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BMRB: 5297

1L5I

1H, 13C, and 15N assignment of the N-terminal, DNA-binding domain of the replication initiation protein from a geminivirus

Authors

Campos-Olivas, R., Louis, J.M., Clerot, J.M., Gronenborn, B., Gronenborn, A.M.

Assembly

N-terminal DNA-binding domain of Rep protein from a geminivirus

Entity

1. N-terminal DNA-binding domain of Rep protein from a geminivirus (polymer, Thiol state: all free), 118 monomers, 13563.13 Da Detail

SGRFSIKAKN YFLTYPKCDL TKENALSQIT NLQTPTNKLF IKICRELHEN GEPHLHILIQ FEGKYNCTNQ RFFDLVSPTR SAHFHPNIQG AKSSSDVKSY IDKDGDVLEW GTFQIDGR

Formula weight

13563.13 Da

Source organism

Tomato yellow leaf curl Sardinia virus

Exptl. method

NMR

Refine. method

torsion angle simulated anhealing

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete

Sequence coverage: 99.2 %, Completeness: 91.3 %, Completeness (bb): 95.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	91.3 % (1300 of 1424)	89.8 % (669 of 745)	92.6 % (510 of 551)	94.5 % (121 of 128)
Backbone	95.6 % (667 of 698)	95.8 % (228 of 238)	95.7 % (332 of 347)	94.7 % (107 of 113)
Sidechain	88.6 % (742 of 837)	87.0 % (441 of 507)	91.1 % (287 of 315)	93.3 % (14 of 15)
Aromatic	72.2 % (104 of 144)	75.0 % (54 of 72)	69.0 % (49 of 71)	100.0 % (1 of 1)
Methyl	100.0 % (112 of 112)	100.0 % (56 of 56)	100.0 % (56 of 56)

1. N-terminal DNA-binding domain of Rep protein from a geminivirus

SGRFSIKAKN YFLTYPKCDL TKENALSQIT NLQTPTNKLF IKICRELHEN GEPHLHILIQ FEGKYNCTNQ RFFDLVSPTR SAHFHPNIQG AKSSSDVKSY IDKDGDVLEW GTFQIDGR

Show sample condition

Sample #1

Temperature 298 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	N-terminal DNA-binding domain of Rep protein from a geminivirus	[10%-13C; U-99% 15N]	0.8 ~ 1.0 mM
2	sodium phosphate		20 mM
3	NaCl		100 mM
4	DTT		1 mM
5	D2O		8 %
6	H2O		92 %

Sample #2

Temperature 298 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
7	N-terminal DNA-binding domain of Rep protein from a geminivirus	[U-13C; U-99% 15N]	0.8 ~ 1.0 mM
8	sodium phosphate		20 mM
9	NaCl		100 mM
10	DTT		1 mM
11	D2O		8 %
12	H2O		92 %

Sample #3

Temperature 298 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
13	N-terminal DNA-binding domain of Rep protein from a geminivirus	[U-10% 13C; U-99% 15N]	0.8 ~ 1.0 mM
14	sodium phosphate		20 mM
15	NaCl		100 mM
16	DTT		1 mM
17	D2O		100 %

Sample #4

Temperature 298 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
18	N-terminal DNA-binding domain of Rep protein from a geminivirus	[U-13C; U-99% 15N]	0.8 ~ 1.0 mM
19	sodium phosphate		20 mM
20	NaCl		100 mM
21	DTT		1 mM
22	D2O		100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	H2O	water signal	4.774 ppm	internal	direct	1.0
¹⁵N	H2O	water signal	0.0 ppm	null	indirect	0.1013291
¹³C	H2O	water signal	0.0 ppm	null	indirect	0.2514495

Referencing offset: 0.14 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	H2O	water signal	4.774 ppm	internal	direct	1.0
¹⁵N	H2O	water signal	0.0 ppm	null	indirect	0.1013291
¹³C	H2O	water signal	0.0 ppm	null	indirect	0.2514495

Referencing offset: 0.14 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	H2O	water signal	4.774 ppm	internal	direct	1.0
¹⁵N	H2O	water signal	0.0 ppm	null	indirect	0.1013291
¹³C	H2O	water signal	0.0 ppm	null	indirect	0.2514495

Referencing offset: -0.04 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	H2O	water signal	4.774 ppm	internal	direct	1.0
¹⁵N	H2O	water signal	0.0 ppm	null	indirect	0.1013291
¹³C	H2O	water signal	0.0 ppm	null	indirect	0.2514495

Referencing offset: 0.33 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 1 models in PDB: 1L2M, Strand ID: A Detail

Coupling constant

84 J values in 1 lists
Temperature 298 (±0.5) K, pH 6.6 (±0.1) Detail

Release date

2002-09-10

Citation

The structure of a replication initiator unites diverse aspects of nucleic acid metabolism
Campos-Olivas, R., Louis, J.M., Clerot, J.M., Gronenborn, B., Gronenborn, A.M.
Proc. Natl. Acad. Sci. U. S. A. (2002), 99, 10310-10315, PubMed 12130667 , DOI 10.1073/pnas.152342699 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Swiss-Prot: P27260 released on 1992-08-01
Title REP_TYCSV Entity Replication-associated protein

Related entities 1. N-terminal DNA-binding domain of Rep protein from a geminivirus, : 1 : 2 : 50 entities Detail

Experiments performed 15 experiments Detail

1 2D 1H-1H NOESY

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DRX (600 MHz)

#4 Model Bruker DMX (750 MHz)

#5 Model Bruker DRX (800 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	N-terminal DNA-binding domain of Rep protein from a geminivirus	[10%-13C; U-99% 15N]	0.8 ~ 1.0 mM
2	sodium phosphate		20 mM
3	NaCl		100 mM
4	DTT		1 mM
5	D2O		8 %
6	H2O		92 %

Sample #2

Temperature 298 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
7	N-terminal DNA-binding domain of Rep protein from a geminivirus	[U-13C; U-99% 15N]	0.8 ~ 1.0 mM
8	sodium phosphate		20 mM
9	NaCl		100 mM
10	DTT		1 mM
11	D2O		8 %
12	H2O		92 %

Sample #3

Temperature 298 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
13	N-terminal DNA-binding domain of Rep protein from a geminivirus	[U-10% 13C; U-99% 15N]	0.8 ~ 1.0 mM
14	sodium phosphate		20 mM
15	NaCl		100 mM
16	DTT		1 mM
17	D2O		100 %

Sample #4

Temperature 298 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
18	N-terminal DNA-binding domain of Rep protein from a geminivirus	[U-13C; U-99% 15N]	0.8 ~ 1.0 mM
19	sodium phosphate		20 mM
20	NaCl		100 mM
21	DTT		1 mM
22	D2O		100 %

2 2D 1H-1H TOCSY

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DRX (600 MHz)

#4 Model Bruker DMX (750 MHz)

#5 Model Bruker DRX (800 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	N-terminal DNA-binding domain of Rep protein from a geminivirus	[10%-13C; U-99% 15N]	0.8 ~ 1.0 mM
2	sodium phosphate		20 mM
3	NaCl		100 mM
4	DTT		1 mM
5	D2O		8 %
6	H2O		92 %

Sample #2

Temperature 298 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
7	N-terminal DNA-binding domain of Rep protein from a geminivirus	[U-13C; U-99% 15N]	0.8 ~ 1.0 mM
8	sodium phosphate		20 mM
9	NaCl		100 mM
10	DTT		1 mM
11	D2O		8 %
12	H2O		92 %

Sample #3

Temperature 298 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
13	N-terminal DNA-binding domain of Rep protein from a geminivirus	[U-10% 13C; U-99% 15N]	0.8 ~ 1.0 mM
14	sodium phosphate		20 mM
15	NaCl		100 mM
16	DTT		1 mM
17	D2O		100 %

Sample #4

Temperature 298 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
18	N-terminal DNA-binding domain of Rep protein from a geminivirus	[U-13C; U-99% 15N]	0.8 ~ 1.0 mM
19	sodium phosphate		20 mM
20	NaCl		100 mM
21	DTT		1 mM
22	D2O		100 %

3 2D 1H-15N HSQC

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DRX (600 MHz)

#4 Model Bruker DMX (750 MHz)

#5 Model Bruker DRX (800 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	N-terminal DNA-binding domain of Rep protein from a geminivirus	[10%-13C; U-99% 15N]	0.8 ~ 1.0 mM
2	sodium phosphate		20 mM
3	NaCl		100 mM
4	DTT		1 mM
5	D2O		8 %
6	H2O		92 %

Sample #2

Temperature 298 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
7	N-terminal DNA-binding domain of Rep protein from a geminivirus	[U-13C; U-99% 15N]	0.8 ~ 1.0 mM
8	sodium phosphate		20 mM
9	NaCl		100 mM
10	DTT		1 mM
11	D2O		8 %
12	H2O		92 %

Sample #3

Temperature 298 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
13	N-terminal DNA-binding domain of Rep protein from a geminivirus	[U-10% 13C; U-99% 15N]	0.8 ~ 1.0 mM
14	sodium phosphate		20 mM
15	NaCl		100 mM
16	DTT		1 mM
17	D2O		100 %

Sample #4

Temperature 298 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
18	N-terminal DNA-binding domain of Rep protein from a geminivirus	[U-13C; U-99% 15N]	0.8 ~ 1.0 mM
19	sodium phosphate		20 mM
20	NaCl		100 mM
21	DTT		1 mM
22	D2O		100 %

4 2D 1H-15N HSQC long range

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DRX (600 MHz)

#4 Model Bruker DMX (750 MHz)

#5 Model Bruker DRX (800 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	N-terminal DNA-binding domain of Rep protein from a geminivirus	[10%-13C; U-99% 15N]	0.8 ~ 1.0 mM
2	sodium phosphate		20 mM
3	NaCl		100 mM
4	DTT		1 mM
5	D2O		8 %
6	H2O		92 %

Sample #2

Temperature 298 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
7	N-terminal DNA-binding domain of Rep protein from a geminivirus	[U-13C; U-99% 15N]	0.8 ~ 1.0 mM
8	sodium phosphate		20 mM
9	NaCl		100 mM
10	DTT		1 mM
11	D2O		8 %
12	H2O		92 %

Sample #3

Temperature 298 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
13	N-terminal DNA-binding domain of Rep protein from a geminivirus	[U-10% 13C; U-99% 15N]	0.8 ~ 1.0 mM
14	sodium phosphate		20 mM
15	NaCl		100 mM
16	DTT		1 mM
17	D2O		100 %

Sample #4

Temperature 298 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
18	N-terminal DNA-binding domain of Rep protein from a geminivirus	[U-13C; U-99% 15N]	0.8 ~ 1.0 mM
19	sodium phosphate		20 mM
20	NaCl		100 mM
21	DTT		1 mM
22	D2O		100 %

5 2D 1H-13C HSQC/HMQC

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DRX (600 MHz)

#4 Model Bruker DMX (750 MHz)

#5 Model Bruker DRX (800 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	N-terminal DNA-binding domain of Rep protein from a geminivirus	[10%-13C; U-99% 15N]	0.8 ~ 1.0 mM
2	sodium phosphate		20 mM
3	NaCl		100 mM
4	DTT		1 mM
5	D2O		8 %
6	H2O		92 %

Sample #2

Temperature 298 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
7	N-terminal DNA-binding domain of Rep protein from a geminivirus	[U-13C; U-99% 15N]	0.8 ~ 1.0 mM
8	sodium phosphate		20 mM
9	NaCl		100 mM
10	DTT		1 mM
11	D2O		8 %
12	H2O		92 %

Sample #3

Temperature 298 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
13	N-terminal DNA-binding domain of Rep protein from a geminivirus	[U-10% 13C; U-99% 15N]	0.8 ~ 1.0 mM
14	sodium phosphate		20 mM
15	NaCl		100 mM
16	DTT		1 mM
17	D2O		100 %

Sample #4

Temperature 298 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
18	N-terminal DNA-binding domain of Rep protein from a geminivirus	[U-13C; U-99% 15N]	0.8 ~ 1.0 mM
19	sodium phosphate		20 mM
20	NaCl		100 mM
21	DTT		1 mM
22	D2O		100 %

6 3D HNCO

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DRX (600 MHz)

#4 Model Bruker DMX (750 MHz)

#5 Model Bruker DRX (800 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	N-terminal DNA-binding domain of Rep protein from a geminivirus	[10%-13C; U-99% 15N]	0.8 ~ 1.0 mM
2	sodium phosphate		20 mM
3	NaCl		100 mM
4	DTT		1 mM
5	D2O		8 %
6	H2O		92 %

Sample #2

Temperature 298 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
7	N-terminal DNA-binding domain of Rep protein from a geminivirus	[U-13C; U-99% 15N]	0.8 ~ 1.0 mM
8	sodium phosphate		20 mM
9	NaCl		100 mM
10	DTT		1 mM
11	D2O		8 %
12	H2O		92 %

Sample #3

Temperature 298 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
13	N-terminal DNA-binding domain of Rep protein from a geminivirus	[U-10% 13C; U-99% 15N]	0.8 ~ 1.0 mM
14	sodium phosphate		20 mM
15	NaCl		100 mM
16	DTT		1 mM
17	D2O		100 %

Sample #4

Temperature 298 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
18	N-terminal DNA-binding domain of Rep protein from a geminivirus	[U-13C; U-99% 15N]	0.8 ~ 1.0 mM
19	sodium phosphate		20 mM
20	NaCl		100 mM
21	DTT		1 mM
22	D2O		100 %

7 3D HNCACB

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DRX (600 MHz)

#4 Model Bruker DMX (750 MHz)

#5 Model Bruker DRX (800 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	N-terminal DNA-binding domain of Rep protein from a geminivirus	[10%-13C; U-99% 15N]	0.8 ~ 1.0 mM
2	sodium phosphate		20 mM
3	NaCl		100 mM
4	DTT		1 mM
5	D2O		8 %
6	H2O		92 %

Sample #2

Temperature 298 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
7	N-terminal DNA-binding domain of Rep protein from a geminivirus	[U-13C; U-99% 15N]	0.8 ~ 1.0 mM
8	sodium phosphate		20 mM
9	NaCl		100 mM
10	DTT		1 mM
11	D2O		8 %
12	H2O		92 %

Sample #3

Temperature 298 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
13	N-terminal DNA-binding domain of Rep protein from a geminivirus	[U-10% 13C; U-99% 15N]	0.8 ~ 1.0 mM
14	sodium phosphate		20 mM
15	NaCl		100 mM
16	DTT		1 mM
17	D2O		100 %

Sample #4

Temperature 298 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
18	N-terminal DNA-binding domain of Rep protein from a geminivirus	[U-13C; U-99% 15N]	0.8 ~ 1.0 mM
19	sodium phosphate		20 mM
20	NaCl		100 mM
21	DTT		1 mM
22	D2O		100 %

8 3D CBCA(CO)NH

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DRX (600 MHz)

#4 Model Bruker DMX (750 MHz)

#5 Model Bruker DRX (800 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	N-terminal DNA-binding domain of Rep protein from a geminivirus	[10%-13C; U-99% 15N]	0.8 ~ 1.0 mM
2	sodium phosphate		20 mM
3	NaCl		100 mM
4	DTT		1 mM
5	D2O		8 %
6	H2O		92 %

Sample #2

Temperature 298 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
7	N-terminal DNA-binding domain of Rep protein from a geminivirus	[U-13C; U-99% 15N]	0.8 ~ 1.0 mM
8	sodium phosphate		20 mM
9	NaCl		100 mM
10	DTT		1 mM
11	D2O		8 %
12	H2O		92 %

Sample #3

Temperature 298 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
13	N-terminal DNA-binding domain of Rep protein from a geminivirus	[U-10% 13C; U-99% 15N]	0.8 ~ 1.0 mM
14	sodium phosphate		20 mM
15	NaCl		100 mM
16	DTT		1 mM
17	D2O		100 %

Sample #4

Temperature 298 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
18	N-terminal DNA-binding domain of Rep protein from a geminivirus	[U-13C; U-99% 15N]	0.8 ~ 1.0 mM
19	sodium phosphate		20 mM
20	NaCl		100 mM
21	DTT		1 mM
22	D2O		100 %

9 3D HNHA

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DRX (600 MHz)

#4 Model Bruker DMX (750 MHz)

#5 Model Bruker DRX (800 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	N-terminal DNA-binding domain of Rep protein from a geminivirus	[10%-13C; U-99% 15N]	0.8 ~ 1.0 mM
2	sodium phosphate		20 mM
3	NaCl		100 mM
4	DTT		1 mM
5	D2O		8 %
6	H2O		92 %

Sample #2

Temperature 298 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
7	N-terminal DNA-binding domain of Rep protein from a geminivirus	[U-13C; U-99% 15N]	0.8 ~ 1.0 mM
8	sodium phosphate		20 mM
9	NaCl		100 mM
10	DTT		1 mM
11	D2O		8 %
12	H2O		92 %

Sample #3

Temperature 298 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
13	N-terminal DNA-binding domain of Rep protein from a geminivirus	[U-10% 13C; U-99% 15N]	0.8 ~ 1.0 mM
14	sodium phosphate		20 mM
15	NaCl		100 mM
16	DTT		1 mM
17	D2O		100 %

Sample #4

Temperature 298 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
18	N-terminal DNA-binding domain of Rep protein from a geminivirus	[U-13C; U-99% 15N]	0.8 ~ 1.0 mM
19	sodium phosphate		20 mM
20	NaCl		100 mM
21	DTT		1 mM
22	D2O		100 %

10 3D HNHB

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DRX (600 MHz)

#4 Model Bruker DMX (750 MHz)

#5 Model Bruker DRX (800 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	N-terminal DNA-binding domain of Rep protein from a geminivirus	[10%-13C; U-99% 15N]	0.8 ~ 1.0 mM
2	sodium phosphate		20 mM
3	NaCl		100 mM
4	DTT		1 mM
5	D2O		8 %
6	H2O		92 %

Sample #2

Temperature 298 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
7	N-terminal DNA-binding domain of Rep protein from a geminivirus	[U-13C; U-99% 15N]	0.8 ~ 1.0 mM
8	sodium phosphate		20 mM
9	NaCl		100 mM
10	DTT		1 mM
11	D2O		8 %
12	H2O		92 %

Sample #3

Temperature 298 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
13	N-terminal DNA-binding domain of Rep protein from a geminivirus	[U-10% 13C; U-99% 15N]	0.8 ~ 1.0 mM
14	sodium phosphate		20 mM
15	NaCl		100 mM
16	DTT		1 mM
17	D2O		100 %

Sample #4

Temperature 298 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
18	N-terminal DNA-binding domain of Rep protein from a geminivirus	[U-13C; U-99% 15N]	0.8 ~ 1.0 mM
19	sodium phosphate		20 mM
20	NaCl		100 mM
21	DTT		1 mM
22	D2O		100 %

11 2D HNCOCG arom

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DRX (600 MHz)

#4 Model Bruker DMX (750 MHz)

#5 Model Bruker DRX (800 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	N-terminal DNA-binding domain of Rep protein from a geminivirus	[10%-13C; U-99% 15N]	0.8 ~ 1.0 mM
2	sodium phosphate		20 mM
3	NaCl		100 mM
4	DTT		1 mM
5	D2O		8 %
6	H2O		92 %

Sample #2

Temperature 298 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
7	N-terminal DNA-binding domain of Rep protein from a geminivirus	[U-13C; U-99% 15N]	0.8 ~ 1.0 mM
8	sodium phosphate		20 mM
9	NaCl		100 mM
10	DTT		1 mM
11	D2O		8 %
12	H2O		92 %

Sample #3

Temperature 298 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
13	N-terminal DNA-binding domain of Rep protein from a geminivirus	[U-10% 13C; U-99% 15N]	0.8 ~ 1.0 mM
14	sodium phosphate		20 mM
15	NaCl		100 mM
16	DTT		1 mM
17	D2O		100 %

Sample #4

Temperature 298 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
18	N-terminal DNA-binding domain of Rep protein from a geminivirus	[U-13C; U-99% 15N]	0.8 ~ 1.0 mM
19	sodium phosphate		20 mM
20	NaCl		100 mM
21	DTT		1 mM
22	D2O		100 %

12 2D HNCO arom

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DRX (600 MHz)

#4 Model Bruker DMX (750 MHz)

#5 Model Bruker DRX (800 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	N-terminal DNA-binding domain of Rep protein from a geminivirus	[10%-13C; U-99% 15N]	0.8 ~ 1.0 mM
2	sodium phosphate		20 mM
3	NaCl		100 mM
4	DTT		1 mM
5	D2O		8 %
6	H2O		92 %

Sample #2

Temperature 298 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
7	N-terminal DNA-binding domain of Rep protein from a geminivirus	[U-13C; U-99% 15N]	0.8 ~ 1.0 mM
8	sodium phosphate		20 mM
9	NaCl		100 mM
10	DTT		1 mM
11	D2O		8 %
12	H2O		92 %

Sample #3

Temperature 298 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
13	N-terminal DNA-binding domain of Rep protein from a geminivirus	[U-10% 13C; U-99% 15N]	0.8 ~ 1.0 mM
14	sodium phosphate		20 mM
15	NaCl		100 mM
16	DTT		1 mM
17	D2O		100 %

Sample #4

Temperature 298 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
18	N-terminal DNA-binding domain of Rep protein from a geminivirus	[U-13C; U-99% 15N]	0.8 ~ 1.0 mM
19	sodium phosphate		20 mM
20	NaCl		100 mM
21	DTT		1 mM
22	D2O		100 %

13 3D 15N-edited NOESY

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DRX (600 MHz)

#4 Model Bruker DMX (750 MHz)

#5 Model Bruker DRX (800 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	N-terminal DNA-binding domain of Rep protein from a geminivirus	[10%-13C; U-99% 15N]	0.8 ~ 1.0 mM
2	sodium phosphate		20 mM
3	NaCl		100 mM
4	DTT		1 mM
5	D2O		8 %
6	H2O		92 %

Sample #2

Temperature 298 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
7	N-terminal DNA-binding domain of Rep protein from a geminivirus	[U-13C; U-99% 15N]	0.8 ~ 1.0 mM
8	sodium phosphate		20 mM
9	NaCl		100 mM
10	DTT		1 mM
11	D2O		8 %
12	H2O		92 %

Sample #3

Temperature 298 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
13	N-terminal DNA-binding domain of Rep protein from a geminivirus	[U-10% 13C; U-99% 15N]	0.8 ~ 1.0 mM
14	sodium phosphate		20 mM
15	NaCl		100 mM
16	DTT		1 mM
17	D2O		100 %

Sample #4

Temperature 298 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
18	N-terminal DNA-binding domain of Rep protein from a geminivirus	[U-13C; U-99% 15N]	0.8 ~ 1.0 mM
19	sodium phosphate		20 mM
20	NaCl		100 mM
21	DTT		1 mM
22	D2O		100 %

14 4D 15N,13C NOESY

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DRX (600 MHz)

#4 Model Bruker DMX (750 MHz)

#5 Model Bruker DRX (800 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	N-terminal DNA-binding domain of Rep protein from a geminivirus	[10%-13C; U-99% 15N]	0.8 ~ 1.0 mM
2	sodium phosphate		20 mM
3	NaCl		100 mM
4	DTT		1 mM
5	D2O		8 %
6	H2O		92 %

Sample #2

Temperature 298 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
7	N-terminal DNA-binding domain of Rep protein from a geminivirus	[U-13C; U-99% 15N]	0.8 ~ 1.0 mM
8	sodium phosphate		20 mM
9	NaCl		100 mM
10	DTT		1 mM
11	D2O		8 %
12	H2O		92 %

Sample #3

Temperature 298 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
13	N-terminal DNA-binding domain of Rep protein from a geminivirus	[U-10% 13C; U-99% 15N]	0.8 ~ 1.0 mM
14	sodium phosphate		20 mM
15	NaCl		100 mM
16	DTT		1 mM
17	D2O		100 %

Sample #4

Temperature 298 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
18	N-terminal DNA-binding domain of Rep protein from a geminivirus	[U-13C; U-99% 15N]	0.8 ~ 1.0 mM
19	sodium phosphate		20 mM
20	NaCl		100 mM
21	DTT		1 mM
22	D2O		100 %

15 4D 13C,13C NOESY

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DRX (600 MHz)

#4 Model Bruker DMX (750 MHz)

#5 Model Bruker DRX (800 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	N-terminal DNA-binding domain of Rep protein from a geminivirus	[10%-13C; U-99% 15N]	0.8 ~ 1.0 mM
2	sodium phosphate		20 mM
3	NaCl		100 mM
4	DTT		1 mM
5	D2O		8 %
6	H2O		92 %

Sample #2

Temperature 298 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
7	N-terminal DNA-binding domain of Rep protein from a geminivirus	[U-13C; U-99% 15N]	0.8 ~ 1.0 mM
8	sodium phosphate		20 mM
9	NaCl		100 mM
10	DTT		1 mM
11	D2O		8 %
12	H2O		92 %

Sample #3

Temperature 298 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
13	N-terminal DNA-binding domain of Rep protein from a geminivirus	[U-10% 13C; U-99% 15N]	0.8 ~ 1.0 mM
14	sodium phosphate		20 mM
15	NaCl		100 mM
16	DTT		1 mM
17	D2O		100 %

Sample #4

Temperature 298 (±0.5) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
18	N-terminal DNA-binding domain of Rep protein from a geminivirus	[U-13C; U-99% 15N]	0.8 ~ 1.0 mM
19	sodium phosphate		20 mM
20	NaCl		100 mM
21	DTT		1 mM
22	D2O		100 %

null

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BMRB: 5297

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Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. N-terminal DNA-binding domain of Rep protein from a geminivirus, : 1 : 2 : 50 entities Detail

Experiments performed 15 experiments Detail

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