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Solution structure and backbone dynamics of beryllofluoride-activated NTRC receiver domain
Authors
Hastings, C.A., Lee, S., Cho, H.S., Yan, D., Kustu, S., Wemmer, D.E.
Assembly
nitrogen regulation protein NR(I)
Entity
1. nitrogen regulation protein NR(I) (polymer, Thiol state: all free), 124 monomers, 13623.50 Da Detail

MQRGIVWVVD DDSSIRWVLE RALAGAGLTC TTFENGNEVL AALASKTPDV LLSDIRMPGM DGLALLKQIK QRHPMLPVII MTAHSDLDAA VSAYQQGAFD YLPKPFDIDE AVALVERAIS HYQE


Formula weight
13623.5 Da
Source organism
Salmonella enterica subsp. enterica serovar Typhimurium
Exptl. method
NMR
Refine. method
distance geometry,
Data set
assigned_chemical_shifts, coupling_constants
Chem. Shift Complete
Sequence coverage: 98.4 %, Completeness: 83.4 %, Completeness (bb): 81.8 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All83.4 % (1173 of 1407)90.9 % (656 of 722)71.6 % (399 of 557)92.2 % (118 of 128)
Backbone81.8 % (599 of 732)95.6 % (238 of 249)68.2 % (249 of 365)94.9 % (112 of 118)
Sidechain85.7 % (679 of 792)87.5 % (414 of 473)83.8 % (259 of 309)60.0 % (6 of 10)
Aromatic71.1 % (64 of 90)86.7 % (39 of 45)53.5 % (23 of 43)100.0 % (2 of 2)
Methyl98.2 % (165 of 168)100.0 % (84 of 84)96.4 % (81 of 84)

1. nitrogen regulation protein NR(I)

MQRGIVWVVD DDSSIRWVLE RALAGAGLTC TTFENGNEVL AALASKTPDV LLSDIRMPGM DGLALLKQIK QRHPMLPVII MTAHSDLDAA VSAYQQGAFD YLPKPFDIDE AVALVERAIS HYQE

Sample

Pressure 1 atm, Temperature 303 K, pH 6.75


#NameIsotope labelingTypeConcentration
9nitrogen regulation protein NR(I)[U-13C; U-15N]1.1 ~ 1.5 mM
10sodium phosphate buffer50 mM
11NaCl50 mM
12BeCl24.4 mM
13MgCl27.2 mM
14NaF29 mM
15H2O95 %
16D2O5 %

LACS Plot; CA
Referencing offset: -0.37 ppm, Outliers: 2 Detail
LACS Plot; CB
Referencing offset: -0.37 ppm, Outliers: 2 Detail
LACS Plot; HA
Referencing offset: -0.03 ppm, Outliers: 1 Detail
Protein Blocks Logo
Calculated from 26 models in PDB: 1KRX, Strand ID: A Detail


Coupling constant
76 J values in 1 lists
Pressure 1 atm, Temperature 303 K, pH 6.75 Detail
Release date
2003-08-26
Citation
High-resolution solution structure of the beryllofluoride-activated NtrC receiver domain
Hastings, C.A., Lee, S., Cho, H.S., Yan, D., Kustu, S., Wemmer, D.E.
Biochemistry (2003), 42, 9081-9090, PubMed 12885241 , DOI 10.1021/bi0273866 ,
Related entities 1. nitrogen regulation protein NR(I), : 1 : 7 : 3 : 285 entities Detail
Experiments performed 9 experiments Detail
nullKeywords two component signal transduction, receiver domain, BeF3, phosphorylation, Bacterial nitrogen regulatory protein