1H, 13C, and 15N Chemical Shift Assignments for the HIF-1alpha CTAD/p300 CH1 Complex
MGSGAHTADP EKRKLIQQQL VLLLHAHKCQ RREQANGEVR QCNLPHCRTM KNVLNHMTHC QSGKSCQVAH CASSRQIISH WKNCTRHDCP VCLPLKNAGD K
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | na | sing | 3:ZN1:ZN | 2:HIS25:NE2 |
2 | na | sing | 3:ZN1:ZN | 2:CYS29:SG |
3 | na | sing | 3:ZN1:ZN | 2:CYS42:SG |
4 | na | sing | 3:ZN1:ZN | 2:CYS47:SG |
5 | na | sing | 3:ZN1:ZN | 2:HIS56:NE2 |
6 | na | sing | 3:ZN1:ZN | 2:CYS60:SG |
7 | na | sing | 3:ZN1:ZN | 2:CYS66:SG |
8 | na | sing | 3:ZN1:ZN | 2:CYS71:SG |
9 | na | sing | 3:ZN1:ZN | 2:HIS80:NE2 |
10 | na | sing | 3:ZN1:ZN | 2:CYS84:SG |
11 | na | sing | 3:ZN1:ZN | 2:CYS89:SG |
12 | na | sing | 3:ZN1:ZN | 2:CYS92:SG |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 33.3 % (540 of 1624) | 30.0 % (257 of 856) | 36.5 % (222 of 608) | 38.1 % (61 of 160) |
Backbone | 37.6 % (318 of 846) | 35.6 % (103 of 289) | 38.3 % (161 of 420) | 39.4 % (54 of 137) |
Sidechain | 29.8 % (272 of 912) | 27.2 % (154 of 567) | 34.5 % (111 of 322) | 30.4 % (7 of 23) |
Aromatic | 10.7 % (6 of 56) | 14.3 % (4 of 28) | 7.4 % (2 of 27) | 0.0 % (0 of 1) |
Methyl | 43.4 % (59 of 136) | 42.6 % (29 of 68) | 44.1 % (30 of 68) |
1. C-terminal activation domain of hypoxia inducible factor-1alpha
GSMDESGLPQ LTSYDCEVNA PIQGSRNLLQ GEELLRALDQ VN2. p300 cysteine/histidine-rich 1 domain
MGSGAHTADP EKRKLIQQQL VLLLHAHKCQ RREQANGEVR QCNLPHCRTM KNVLNHMTHC QSGKSCQVAH CASSRQIISH WKNCTRHDCP VCLPLKNAGD KTemperature 298 (±1) K, pH 6.0 (±0.2)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | C-terminal activation domain of hypoxia inducible factor-1alpha | 1.0 mM | ||
2 | p300 cysteine/histidine-rich 1 domain | 1.0 mM | ||
3 | ZnSO4 | 0.1 mM | ||
4 | DTT | 1 mM | ||
5 | NaCl | 100 mM |
Temperature 298 (±1) K, pH 6.0 (±0.2)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
6 | C-terminal activation domain of hypoxia inducible factor-1alpha | [U-15N] | 1.0 mM | |
7 | p300 cysteine/histidine-rich 1 domain | [U-15N] | 1.0 mM | |
8 | ZnSO4 | 0.1 mM | ||
9 | DTT | 1 mM | ||
10 | NaCl | 100 mM |
Temperature 298 (±1) K, pH 6.0 (±0.2)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
11 | C-terminal activation domain of hypoxia inducible factor-1alpha | [U-13C; U-15N] | 1.0 mM | |
12 | p300 cysteine/histidine-rich 1 domain | [U-13C; U-15N] | 1.0 mM | |
13 | ZnSO4 | 0.1 mM | ||
14 | DTT | 1 mM | ||
15 | NaCl | 100 mM |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 48.1 % (1563 of 3248) | 44.3 % (759 of 1712) | 52.5 % (638 of 1216) | 51.9 % (166 of 320) |
Backbone | 53.8 % (911 of 1692) | 52.1 % (301 of 578) | 54.6 % (459 of 840) | 55.1 % (151 of 274) |
Sidechain | 43.8 % (799 of 1824) | 40.4 % (458 of 1134) | 50.6 % (326 of 644) | 32.6 % (15 of 46) |
Aromatic | 20.5 % (23 of 112) | 35.7 % (20 of 56) | 3.7 % (2 of 54) | 50.0 % (1 of 2) |
Methyl | 59.2 % (161 of 272) | 58.8 % (80 of 136) | 59.6 % (81 of 136) |
1. C-terminal activation domain of hypoxia inducible factor-1alpha
GSMDESGLPQ LTSYDCEVNA PIQGSRNLLQ GEELLRALDQ VN2. p300 cysteine/histidine-rich 1 domain
MGSGAHTADP EKRKLIQQQL VLLLHAHKCQ RREQANGEVR QCNLPHCRTM KNVLNHMTHC QSGKSCQVAH CASSRQIISH WKNCTRHDCP VCLPLKNAGD KTemperature 298 (±1) K, pH 6.0 (±0.2)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | C-terminal activation domain of hypoxia inducible factor-1alpha | 1.0 mM | ||
2 | p300 cysteine/histidine-rich 1 domain | 1.0 mM | ||
3 | ZnSO4 | 0.1 mM | ||
4 | DTT | 1 mM | ||
5 | NaCl | 100 mM |
Temperature 298 (±1) K, pH 6.0 (±0.2)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
6 | C-terminal activation domain of hypoxia inducible factor-1alpha | [U-15N] | 1.0 mM | |
7 | p300 cysteine/histidine-rich 1 domain | [U-15N] | 1.0 mM | |
8 | ZnSO4 | 0.1 mM | ||
9 | DTT | 1 mM | ||
10 | NaCl | 100 mM |
Temperature 298 (±1) K, pH 6.0 (±0.2)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
11 | C-terminal activation domain of hypoxia inducible factor-1alpha | [U-13C; U-15N] | 1.0 mM | |
12 | p300 cysteine/histidine-rich 1 domain | [U-13C; U-15N] | 1.0 mM | |
13 | ZnSO4 | 0.1 mM | ||
14 | DTT | 1 mM | ||
15 | NaCl | 100 mM |