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BMRB: 5306

1L3E

1H, 13C, and 15N Chemical Shift Assignments for the HIF-1alpha CTAD/p300 CH1 Complex

Authors

Freedman, S.J., Sun, Z.J., Poy, F., Kung, A.L., Livingston, D.M., Wagner, G., Eck, M.J.

Assembly

hypoxia inducible factor-1alpha/p300 complex

Entity

1. C-terminal activation domain of hypoxia inducible factor-1alpha (polymer, Thiol state: all free), 42 monomers, 4561.970 Da Detail

GSMDESGLPQ LTSYDCEVNA PIQGSRNLLQ GEELLRALDQ VN

2. p300 cysteine/histidine-rich 1 domain (polymer, Thiol state: all other bound), 101 monomers, 11401.09 Da Detail

MGSGAHTADP EKRKLIQQQL VLLLHAHKCQ RREQANGEVR QCNLPHCRTM KNVLNHMTHC QSGKSCQVAH CASSRQIISH WKNCTRHDCP VCLPLKNAGD K

3. ZN (non-polymer), 65.409 × 3 Da

Total weight

16159.287 Da

Max. entity weight

11401.09 Da

Entity Connection

na 12 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	3:ZN1:ZN	2:HIS25:NE2
2	na	sing	3:ZN1:ZN	2:CYS29:SG
3	na	sing	3:ZN1:ZN	2:CYS42:SG
4	na	sing	3:ZN1:ZN	2:CYS47:SG
5	na	sing	3:ZN1:ZN	2:HIS56:NE2
6	na	sing	3:ZN1:ZN	2:CYS60:SG
7	na	sing	3:ZN1:ZN	2:CYS66:SG
8	na	sing	3:ZN1:ZN	2:CYS71:SG
9	na	sing	3:ZN1:ZN	2:HIS80:NE2
10	na	sing	3:ZN1:ZN	2:CYS84:SG
11	na	sing	3:ZN1:ZN	2:CYS89:SG
12	na	sing	3:ZN1:ZN	2:CYS92:SG

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

distance geometry,

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 40.6 %, Completeness: 33.3 %, Completeness (bb): 37.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	33.3 % (540 of 1624)	30.0 % (257 of 856)	36.5 % (222 of 608)	38.1 % (61 of 160)
Backbone	37.6 % (318 of 846)	35.6 % (103 of 289)	38.3 % (161 of 420)	39.4 % (54 of 137)
Sidechain	29.8 % (272 of 912)	27.2 % (154 of 567)	34.5 % (111 of 322)	30.4 % (7 of 23)
Aromatic	10.7 % (6 of 56)	14.3 % (4 of 28)	7.4 % (2 of 27)	0.0 % (0 of 1)
Methyl	43.4 % (59 of 136)	42.6 % (29 of 68)	44.1 % (30 of 68)

1. C-terminal activation domain of hypoxia inducible factor-1alpha

GSMDESGLPQ LTSYDCEVNA PIQGSRNLLQ GEELLRALDQ VN

2. p300 cysteine/histidine-rich 1 domain

MGSGAHTADP EKRKLIQQQL VLLLHAHKCQ RREQANGEVR QCNLPHCRTM KNVLNHMTHC QSGKSCQVAH CASSRQIISH WKNCTRHDCP VCLPLKNAGD K

Show sample condition

Sample #1

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Concentration
1	C-terminal activation domain of hypoxia inducible factor-1alpha	1.0 mM
2	p300 cysteine/histidine-rich 1 domain	1.0 mM
3	ZnSO4	0.1 mM
4	DTT	1 mM
5	NaCl	100 mM

Sample #2

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Concentration
6	C-terminal activation domain of hypoxia inducible factor-1alpha	[U-15N]	1.0 mM
7	p300 cysteine/histidine-rich 1 domain	[U-15N]	1.0 mM
8	ZnSO4		0.1 mM
9	DTT		1 mM
10	NaCl		100 mM

Sample #3

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Concentration
11	C-terminal activation domain of hypoxia inducible factor-1alpha	[U-13C; U-15N]	1.0 mM
12	p300 cysteine/histidine-rich 1 domain	[U-13C; U-15N]	1.0 mM
13	ZnSO4		0.1 mM
14	DTT		1 mM
15	NaCl		100 mM

Chem. Shift Complete2

Sequence coverage: 74.8 %, Completeness: 48.1 %, Completeness (bb): 53.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	48.1 % (1563 of 3248)	44.3 % (759 of 1712)	52.5 % (638 of 1216)	51.9 % (166 of 320)
Backbone	53.8 % (911 of 1692)	52.1 % (301 of 578)	54.6 % (459 of 840)	55.1 % (151 of 274)
Sidechain	43.8 % (799 of 1824)	40.4 % (458 of 1134)	50.6 % (326 of 644)	32.6 % (15 of 46)
Aromatic	20.5 % (23 of 112)	35.7 % (20 of 56)	3.7 % (2 of 54)	50.0 % (1 of 2)
Methyl	59.2 % (161 of 272)	58.8 % (80 of 136)	59.6 % (81 of 136)

1. C-terminal activation domain of hypoxia inducible factor-1alpha

GSMDESGLPQ LTSYDCEVNA PIQGSRNLLQ GEELLRALDQ VN

2. p300 cysteine/histidine-rich 1 domain

MGSGAHTADP EKRKLIQQQL VLLLHAHKCQ RREQANGEVR QCNLPHCRTM KNVLNHMTHC QSGKSCQVAH CASSRQIISH WKNCTRHDCP VCLPLKNAGD K

Show sample condition

Sample #1

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Concentration
1	C-terminal activation domain of hypoxia inducible factor-1alpha	1.0 mM
2	p300 cysteine/histidine-rich 1 domain	1.0 mM
3	ZnSO4	0.1 mM
4	DTT	1 mM
5	NaCl	100 mM

Sample #2

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Concentration
6	C-terminal activation domain of hypoxia inducible factor-1alpha	[U-15N]	1.0 mM
7	p300 cysteine/histidine-rich 1 domain	[U-15N]	1.0 mM
8	ZnSO4		0.1 mM
9	DTT		1 mM
10	NaCl		100 mM

Sample #3

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Concentration
11	C-terminal activation domain of hypoxia inducible factor-1alpha	[U-13C; U-15N]	1.0 mM
12	p300 cysteine/histidine-rich 1 domain	[U-13C; U-15N]	1.0 mM
13	ZnSO4		0.1 mM
14	DTT		1 mM
15	NaCl		100 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.18 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.18 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.03 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.38 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 17 models in PDB: 1L3E, Strand ID: A, B Detail

Release date

2002-05-14

Citation

Structural basis for recruitment of CBP/p300 by hypoxia-inducible factor-1 alpha
Freedman, S.J., Sun, Z.J., Poy, F., Kung, A.L., Livingston, D.M., Wagner, G., Eck, M.J.
Proc. Natl. Acad. Sci. U. S. A. (2002), 99, 5367-5372, PubMed 11959990 , DOI 10.1073/pnas.082117899 , Abstract

Related entities 1. C-terminal activation domain of hypoxia inducible factor-1alpha, : 9 : 16 entities Detail

Related entities 2. p300 cysteine/histidine-rich 1 domain, : 5 : 49 entities Detail

Interaction partners 1. C-terminal activation domain of hypoxia inducible factor-1alpha, : 64 : 1 interactors Detail

More details for 65 interactors identified by 39 citations

Interaction partners 2. p300 cysteine/histidine-rich 1 domain, : 133 : 1 interactors Detail

More details for 134 interactors identified by 86 citations

Experiments performed 1 experiments Detail

nullKeywords protein-protein complex, NMR

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Found 1 Document (4.346 seconds)

BMRB: 5306

Sample #1

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Sample #3

Sample #1

Sample #2

Sample #3

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. C-terminal activation domain of hypoxia inducible factor-1alpha, : 9 : 16 entities Detail

Related entities 2. p300 cysteine/histidine-rich 1 domain, : 5 : 49 entities Detail

Interaction partners 1. C-terminal activation domain of hypoxia inducible factor-1alpha, : 64 : 1 interactors Detail

Interaction partners 2. p300 cysteine/histidine-rich 1 domain, : 133 : 1 interactors Detail

Experiments performed 1 experiments Detail

Instrument

Sample #1

Sample #2

Sample #3