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Found 1 Document (1.917 seconds)

BMRB: 5309

1M3V

1H, 15N and 13C assignments of FLIN4, an intramolecular LMO4:ldb1 complex

Authors

Deane, J.E., Visvader, J.E., Mackay, J.P., Matthews, J.M.

Assembly

Fusion of the LIM binding domain of Ldb1 and the N-terminal LIM domain of LMO4

Entity

1. engineered intramolecular protein complex (polymer, Thiol state: all other bound), 122 monomers, 13004.39 Da Detail

GSLSWKRCAG CGGKIADRFL LYAMDSYWHS RCLKCSSCQA QLGDIGTSSY TKSGMILCRN DYIRLFGNSG AGGSGGHMGS GGDVMVVGEP TLMGGEFGDE DERLITRLEN TQFDAANGID DE

2. ZN (non-polymer), 65.409 × 2 Da

Total weight

13135.208 Da

Max. entity weight

13004.39 Da

Entity Connection

na 8 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS8:SG	2:ZN1:ZN
2	na	sing	1:CYS11:SG	2:ZN1:ZN
3	na	sing	1:HIS29:ND1	2:ZN1:ZN
4	na	sing	1:CYS32:SG	2:ZN1:ZN
5	na	sing	1:CYS35:SG	2:ZN1:ZN
6	na	sing	1:CYS38:SG	2:ZN1:ZN
7	na	sing	1:CYS58:SG	2:ZN1:ZN
8	na	sing	1:ASP61:OD1	2:ZN1:ZN

Source organism

Mus musculus

Exptl. method

NMR

Refine. method

simulated annealing molecular dynamics torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.2 %, Completeness: 86.2 %, Completeness (bb): 92.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	86.2 % (1123 of 1303)	89.6 % (602 of 672)	80.6 % (404 of 501)	90.0 % (117 of 130)
Backbone	92.9 % (678 of 730)	88.6 % (234 of 264)	95.4 % (329 of 345)	95.0 % (115 of 121)
Sidechain	80.7 % (544 of 674)	90.2 % (368 of 408)	67.7 % (174 of 257)	22.2 % (2 of 9)
Aromatic	45.2 % (47 of 104)	86.5 % (45 of 52)	0.0 % (0 of 50)	100.0 % (2 of 2)
Methyl	82.0 % (82 of 100)	96.0 % (48 of 50)	68.0 % (34 of 50)

1. engineered intramolecular protein complex

GSLSWKRCAG CGGKIADRFL LYAMDSYWHS RCLKCSSCQA QLGDIGTSSY TKSGMILCRN DYIRLFGNSG AGGSGGHMGS GGDVMVVGEP TLMGGEFGDE DERLITRLEN TQFDAANGID DE

Show sample condition

Sample #1

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	engineered intramolecular protein complex			0.3 ~ 0.8 mM

Sample #2

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	engineered intramolecular protein complex	[U-95% 15N]		0.3 ~ 0.8 mM

Sample #3

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	engineered intramolecular protein complex	[U-95% 13C; U-95% 15N]		0.3 ~ 0.8 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.16 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.16 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.05 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.57 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1M3V, Strand ID: A Detail

Release date

2002-08-21

Citation

1H, 15N and 13C assignments of FLIN4, an intramolecular LMO4:ldb1 complex
Deane, J.E., Visvader, J.E., Mackay, J.P., Matthews, J.M.
J. Biomol. NMR (2002), 23, 165-166, PubMed 12153047 , DOI: , Abstract

Related entities 1. engineered intramolecular protein complex, : 1 : 24 entities Detail

Experiments performed 15 experiments Detail

1 1H TOCSY

Instrument

Model Bruker DMX (600 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	engineered intramolecular protein complex			0.3 ~ 0.8 mM

Sample #2

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	engineered intramolecular protein complex	[U-95% 15N]		0.3 ~ 0.8 mM

Sample #3

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	engineered intramolecular protein complex	[U-95% 13C; U-95% 15N]		0.3 ~ 0.8 mM

2 1H NOESY

Instrument

Model Bruker DMX (600 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	engineered intramolecular protein complex			0.3 ~ 0.8 mM

Sample #2

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	engineered intramolecular protein complex	[U-95% 15N]		0.3 ~ 0.8 mM

Sample #3

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	engineered intramolecular protein complex	[U-95% 13C; U-95% 15N]		0.3 ~ 0.8 mM

3 1H DQF-COSY

Instrument

Model Bruker DMX (600 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	engineered intramolecular protein complex			0.3 ~ 0.8 mM

Sample #2

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	engineered intramolecular protein complex	[U-95% 15N]		0.3 ~ 0.8 mM

Sample #3

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	engineered intramolecular protein complex	[U-95% 13C; U-95% 15N]		0.3 ~ 0.8 mM

4 1H-15N HSQC

Instrument

Model Bruker DMX (600 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	engineered intramolecular protein complex			0.3 ~ 0.8 mM

Sample #2

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	engineered intramolecular protein complex	[U-95% 15N]		0.3 ~ 0.8 mM

Sample #3

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	engineered intramolecular protein complex	[U-95% 13C; U-95% 15N]		0.3 ~ 0.8 mM

5 HNHA

Instrument

Model Bruker DMX (600 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	engineered intramolecular protein complex			0.3 ~ 0.8 mM

Sample #2

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	engineered intramolecular protein complex	[U-95% 15N]		0.3 ~ 0.8 mM

Sample #3

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	engineered intramolecular protein complex	[U-95% 13C; U-95% 15N]		0.3 ~ 0.8 mM

6 HNCA

Instrument

Model Bruker DMX (600 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	engineered intramolecular protein complex			0.3 ~ 0.8 mM

Sample #2

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	engineered intramolecular protein complex	[U-95% 15N]		0.3 ~ 0.8 mM

Sample #3

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	engineered intramolecular protein complex	[U-95% 13C; U-95% 15N]		0.3 ~ 0.8 mM

7 HN(CO)CA

Instrument

Model Bruker DMX (600 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	engineered intramolecular protein complex			0.3 ~ 0.8 mM

Sample #2

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	engineered intramolecular protein complex	[U-95% 15N]		0.3 ~ 0.8 mM

Sample #3

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	engineered intramolecular protein complex	[U-95% 13C; U-95% 15N]		0.3 ~ 0.8 mM

8 HNCACB

Instrument

Model Bruker DMX (600 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	engineered intramolecular protein complex			0.3 ~ 0.8 mM

Sample #2

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	engineered intramolecular protein complex	[U-95% 15N]		0.3 ~ 0.8 mM

Sample #3

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	engineered intramolecular protein complex	[U-95% 13C; U-95% 15N]		0.3 ~ 0.8 mM

9 CBCA(CO)NH

Instrument

Model Bruker DMX (600 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	engineered intramolecular protein complex			0.3 ~ 0.8 mM

Sample #2

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	engineered intramolecular protein complex	[U-95% 15N]		0.3 ~ 0.8 mM

Sample #3

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	engineered intramolecular protein complex	[U-95% 13C; U-95% 15N]		0.3 ~ 0.8 mM

10 HNCO

Instrument

Model Bruker DMX (600 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	engineered intramolecular protein complex			0.3 ~ 0.8 mM

Sample #2

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	engineered intramolecular protein complex	[U-95% 15N]		0.3 ~ 0.8 mM

Sample #3

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	engineered intramolecular protein complex	[U-95% 13C; U-95% 15N]		0.3 ~ 0.8 mM

11 HN(CA)CO

Instrument

Model Bruker DMX (600 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	engineered intramolecular protein complex			0.3 ~ 0.8 mM

Sample #2

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	engineered intramolecular protein complex	[U-95% 15N]		0.3 ~ 0.8 mM

Sample #3

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	engineered intramolecular protein complex	[U-95% 13C; U-95% 15N]		0.3 ~ 0.8 mM

12 CC(CO)NH-TOCSY

Instrument

Model Bruker DMX (600 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	engineered intramolecular protein complex			0.3 ~ 0.8 mM

Sample #2

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	engineered intramolecular protein complex	[U-95% 15N]		0.3 ~ 0.8 mM

Sample #3

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	engineered intramolecular protein complex	[U-95% 13C; U-95% 15N]		0.3 ~ 0.8 mM

13 HCC(CO)NH-TOCSY

Instrument

Model Bruker DMX (600 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	engineered intramolecular protein complex			0.3 ~ 0.8 mM

Sample #2

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	engineered intramolecular protein complex	[U-95% 15N]		0.3 ~ 0.8 mM

Sample #3

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	engineered intramolecular protein complex	[U-95% 13C; U-95% 15N]		0.3 ~ 0.8 mM

14 HCCH-TOCSY

Instrument

Model Bruker DMX (600 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	engineered intramolecular protein complex			0.3 ~ 0.8 mM

Sample #2

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	engineered intramolecular protein complex	[U-95% 15N]		0.3 ~ 0.8 mM

Sample #3

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	engineered intramolecular protein complex	[U-95% 13C; U-95% 15N]		0.3 ~ 0.8 mM

15 15N-NOESY-HSQC

Instrument

Model Bruker DMX (600 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	engineered intramolecular protein complex			0.3 ~ 0.8 mM

Sample #2

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	engineered intramolecular protein complex	[U-95% 15N]		0.3 ~ 0.8 mM

Sample #3

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	engineered intramolecular protein complex	[U-95% 13C; U-95% 15N]		0.3 ~ 0.8 mM

null

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Found 1 Document (1.917 seconds)

BMRB: 5309

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Chemical shift reference

Chemical shift reference

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Chemical shift reference

Related entities 1. engineered intramolecular protein complex, : 1 : 24 entities Detail

Experiments performed 15 experiments Detail

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