BMRBj - BMREntryMaster

Found 1 Document (1.675 seconds)

BMRB: 5309

1M3V

1H, 15N and 13C assignments of FLIN4, an intramolecular LMO4:ldb1 complex

Authors

Deane, J.E., Visvader, J.E., Mackay, J.P., Matthews, J.M.

Assembly

Fusion of the LIM binding domain of Ldb1 and the N-terminal LIM domain of LMO4

Entity

1. engineered intramolecular protein complex (polymer, Thiol state: all other bound), 122 monomers, 13004.39 Da Detail

GSLSWKRCAG CGGKIADRFL LYAMDSYWHS RCLKCSSCQA QLGDIGTSSY TKSGMILCRN DYIRLFGNSG AGGSGGHMGS GGDVMVVGEP TLMGGEFGDE DERLITRLEN TQFDAANGID DE

2. ZN (non-polymer), 65.409 × 2 Da

Total weight

13135.208 Da

Max. entity weight

13004.39 Da

Entity Connection

na 8 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS8:SG	2:ZN1:ZN
2	na	sing	1:CYS11:SG	2:ZN1:ZN
3	na	sing	1:HIS29:ND1	2:ZN1:ZN
4	na	sing	1:CYS32:SG	2:ZN1:ZN
5	na	sing	1:CYS35:SG	2:ZN1:ZN
6	na	sing	1:CYS38:SG	2:ZN1:ZN
7	na	sing	1:CYS58:SG	2:ZN1:ZN
8	na	sing	1:ASP61:OD1	2:ZN1:ZN

Source organism

Mus musculus

Exptl. method

NMR

Refine. method

simulated annealing molecular dynamics torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.2 %, Completeness: 86.2 %, Completeness (bb): 92.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	86.2 % (1123 of 1303)	89.6 % (602 of 672)	80.6 % (404 of 501)	90.0 % (117 of 130)
Backbone	92.9 % (678 of 730)	88.6 % (234 of 264)	95.4 % (329 of 345)	95.0 % (115 of 121)
Sidechain	80.7 % (544 of 674)	90.2 % (368 of 408)	67.7 % (174 of 257)	22.2 % (2 of 9)
Aromatic	45.2 % (47 of 104)	86.5 % (45 of 52)	0.0 % (0 of 50)	100.0 % (2 of 2)
Methyl	82.0 % (82 of 100)	96.0 % (48 of 50)	68.0 % (34 of 50)

1. engineered intramolecular protein complex

GSLSWKRCAG CGGKIADRFL LYAMDSYWHS RCLKCSSCQA QLGDIGTSSY TKSGMILCRN DYIRLFGNSG AGGSGGHMGS GGDVMVVGEP TLMGGEFGDE DERLITRLEN TQFDAANGID DE

Show sample condition

Sample #1

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	engineered intramolecular protein complex			0.3 ~ 0.8 mM

Sample #2

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	engineered intramolecular protein complex	[U-95% 15N]		0.3 ~ 0.8 mM

Sample #3

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	engineered intramolecular protein complex	[U-95% 13C; U-95% 15N]		0.3 ~ 0.8 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.16 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.16 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.05 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.57 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1M3V, Strand ID: A Detail

Release date

2002-08-21

Citation

1H, 15N and 13C assignments of FLIN4, an intramolecular LMO4:ldb1 complex
Deane, J.E., Visvader, J.E., Mackay, J.P., Matthews, J.M.
J. Biomol. NMR (2002), 23, 165-166, PubMed 12153047 , DOI: , Abstract

Related entities 1. engineered intramolecular protein complex, : 1 : 24 entities Detail

Experiments performed 15 experiments Detail

1 1H TOCSY

Instrument

Model Bruker DMX (600 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	engineered intramolecular protein complex			0.3 ~ 0.8 mM

Sample #2

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	engineered intramolecular protein complex	[U-95% 15N]		0.3 ~ 0.8 mM

Sample #3

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	engineered intramolecular protein complex	[U-95% 13C; U-95% 15N]		0.3 ~ 0.8 mM

2 1H NOESY

Instrument

Model Bruker DMX (600 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	engineered intramolecular protein complex			0.3 ~ 0.8 mM

Sample #2

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	engineered intramolecular protein complex	[U-95% 15N]		0.3 ~ 0.8 mM

Sample #3

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	engineered intramolecular protein complex	[U-95% 13C; U-95% 15N]		0.3 ~ 0.8 mM

3 1H DQF-COSY

Instrument

Model Bruker DMX (600 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	engineered intramolecular protein complex			0.3 ~ 0.8 mM

Sample #2

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	engineered intramolecular protein complex	[U-95% 15N]		0.3 ~ 0.8 mM

Sample #3

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	engineered intramolecular protein complex	[U-95% 13C; U-95% 15N]		0.3 ~ 0.8 mM

4 1H-15N HSQC

Instrument

Model Bruker DMX (600 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	engineered intramolecular protein complex			0.3 ~ 0.8 mM

Sample #2

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	engineered intramolecular protein complex	[U-95% 15N]		0.3 ~ 0.8 mM

Sample #3

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	engineered intramolecular protein complex	[U-95% 13C; U-95% 15N]		0.3 ~ 0.8 mM

5 HNHA

Instrument

Model Bruker DMX (600 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	engineered intramolecular protein complex			0.3 ~ 0.8 mM

Sample #2

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	engineered intramolecular protein complex	[U-95% 15N]		0.3 ~ 0.8 mM

Sample #3

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	engineered intramolecular protein complex	[U-95% 13C; U-95% 15N]		0.3 ~ 0.8 mM

6 HNCA

Instrument

Model Bruker DMX (600 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	engineered intramolecular protein complex			0.3 ~ 0.8 mM

Sample #2

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	engineered intramolecular protein complex	[U-95% 15N]		0.3 ~ 0.8 mM

Sample #3

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	engineered intramolecular protein complex	[U-95% 13C; U-95% 15N]		0.3 ~ 0.8 mM

7 HN(CO)CA

Instrument

Model Bruker DMX (600 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	engineered intramolecular protein complex			0.3 ~ 0.8 mM

Sample #2

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	engineered intramolecular protein complex	[U-95% 15N]		0.3 ~ 0.8 mM

Sample #3

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	engineered intramolecular protein complex	[U-95% 13C; U-95% 15N]		0.3 ~ 0.8 mM

8 HNCACB

Instrument

Model Bruker DMX (600 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	engineered intramolecular protein complex			0.3 ~ 0.8 mM

Sample #2

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	engineered intramolecular protein complex	[U-95% 15N]		0.3 ~ 0.8 mM

Sample #3

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	engineered intramolecular protein complex	[U-95% 13C; U-95% 15N]		0.3 ~ 0.8 mM

9 CBCA(CO)NH

Instrument

Model Bruker DMX (600 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	engineered intramolecular protein complex			0.3 ~ 0.8 mM

Sample #2

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	engineered intramolecular protein complex	[U-95% 15N]		0.3 ~ 0.8 mM

Sample #3

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	engineered intramolecular protein complex	[U-95% 13C; U-95% 15N]		0.3 ~ 0.8 mM

10 HNCO

Instrument

Model Bruker DMX (600 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	engineered intramolecular protein complex			0.3 ~ 0.8 mM

Sample #2

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	engineered intramolecular protein complex	[U-95% 15N]		0.3 ~ 0.8 mM

Sample #3

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	engineered intramolecular protein complex	[U-95% 13C; U-95% 15N]		0.3 ~ 0.8 mM

11 HN(CA)CO

Instrument

Model Bruker DMX (600 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	engineered intramolecular protein complex			0.3 ~ 0.8 mM

Sample #2

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	engineered intramolecular protein complex	[U-95% 15N]		0.3 ~ 0.8 mM

Sample #3

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	engineered intramolecular protein complex	[U-95% 13C; U-95% 15N]		0.3 ~ 0.8 mM

12 CC(CO)NH-TOCSY

Instrument

Model Bruker DMX (600 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	engineered intramolecular protein complex			0.3 ~ 0.8 mM

Sample #2

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	engineered intramolecular protein complex	[U-95% 15N]		0.3 ~ 0.8 mM

Sample #3

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	engineered intramolecular protein complex	[U-95% 13C; U-95% 15N]		0.3 ~ 0.8 mM

13 HCC(CO)NH-TOCSY

Instrument

Model Bruker DMX (600 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	engineered intramolecular protein complex			0.3 ~ 0.8 mM

Sample #2

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	engineered intramolecular protein complex	[U-95% 15N]		0.3 ~ 0.8 mM

Sample #3

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	engineered intramolecular protein complex	[U-95% 13C; U-95% 15N]		0.3 ~ 0.8 mM

14 HCCH-TOCSY

Instrument

Model Bruker DMX (600 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	engineered intramolecular protein complex			0.3 ~ 0.8 mM

Sample #2

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	engineered intramolecular protein complex	[U-95% 15N]		0.3 ~ 0.8 mM

Sample #3

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	engineered intramolecular protein complex	[U-95% 13C; U-95% 15N]		0.3 ~ 0.8 mM

15 15N-NOESY-HSQC

Instrument

Model Bruker DMX (600 MHz)

Sample #1

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	engineered intramolecular protein complex			0.3 ~ 0.8 mM

Sample #2

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	engineered intramolecular protein complex	[U-95% 15N]		0.3 ~ 0.8 mM

Sample #3

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	engineered intramolecular protein complex	[U-95% 13C; U-95% 15N]		0.3 ~ 0.8 mM

null

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (1.675 seconds)

BMRB: 5309

Sample #1

Sample #2

Sample #3

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. engineered intramolecular protein complex, : 1 : 24 entities Detail

Experiments performed 15 experiments Detail

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1

Sample #2

Sample #3

Instrument

Sample #1