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Found 1 Document (1.83 seconds)

BMRB: 5312

1NEE

1H, 13C and 15N chemical shift assignments of aIF2beta from Methanobacterium themoautotrophicum

Authors

Gutierrez, P.A., Coillet-Matillon, S., Arrowsmith, C., Gehring, K.B.

Assembly

aIF2beta

Entity

1. translation initiation factor 2 beta (polymer, Thiol state: all other bound), 135 monomers, 15586.78 Da Detail

MDDYEKLLER AIDQLPPEVF ETKRFEVPKA YSVIQGNRTF IQNFREVADA LNRDPQHLLK FLLRELGTAG NLEGGRAILQ GKFTHFLINE RIEDYVNKFV ICHECNRPDT RIIREGRISL LKCEACGAKA PLKNV

2. ZN (non-polymer), 65.409 Da

Total weight

15652.189 Da

Max. entity weight

15586.78 Da

Entity Connection

na 4 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS102:SG	2:ZN1:ZN
2	na	sing	1:CYS105:SG	2:ZN1:ZN
3	na	sing	1:CYS123:SG	2:ZN1:ZN
4	na	sing	1:CYS126:SG	2:ZN1:ZN

Source organism

Methanothermobacter thermautotrophicus

Exptl. method

NMR

Refine. method

The structures are based on 1142 NOE-derived constraints, 118 dihedral angle restraints, 39 hydrogen bonds and 58 NH residual dipolar couplings

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.3 %, Completeness: 55.3 %, Completeness (bb): 79.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	55.3 % (903 of 1634)	53.6 % (461 of 860)	50.5 % (319 of 632)	86.6 % (123 of 142)
Backbone	79.4 % (634 of 798)	90.1 % (245 of 272)	67.3 % (267 of 397)	94.6 % (122 of 129)
Sidechain	40.8 % (393 of 963)	36.7 % (216 of 588)	48.6 % (176 of 362)	7.7 % (1 of 13)
Aromatic	2.6 % (3 of 116)	5.2 % (3 of 58)	0.0 % (0 of 58)
Methyl	55.8 % (87 of 156)	60.3 % (47 of 78)	51.3 % (40 of 78)

1. translation initiation factor 2 beta

MDDYEKLLER AIDQLPPEVF ETKRFEVPKA YSVIQGNRTF IQNFREVADA LNRDPQHLLK FLLRELGTAG NLEGGRAILQ GKFTHFLINE RIEDYVNKFV ICHECNRPDT RIIREGRISL LKCEACGAKA PLKNV

Show sample condition

Sample #1

Temperature 310 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	translation initiation factor 2 beta	[U-13C; U-15N]		1.0 ~ 2.0 mM
2	ZINC (II) ION			51 mM

Sample #2

Temperature 310 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	translation initiation factor 2 beta	[U-15N]		1.0 ~ 2.0 mM
4	ZINC (II) ION			51 mM

Protein Blocks Logo

Calculated from 20 models in PDB: 1NEE, Strand ID: A Detail

Release date

2003-02-19

Citation

Zinc is required for structural stability of the C-terminus of archaeal translation initiation factor aIF2beta
Gutierrez, P.A., Coillet-Matillon, S., Arrowsmith, C., Gehring, K.B.
FEBS Lett. (2002), 517, 155-158, PubMed 12062427 , DOI 10.1016/s0014-5793(02)02610-8 , Abstract

Related entities 1. translation initiation factor 2 beta, : 1 : 1 : 119 entities Detail

Experiments performed 6 experiments Detail

1 1H-15N NOESY

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Varian UNITYplus (800 MHz)

Sample #1

Temperature 310 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	translation initiation factor 2 beta	[U-13C; U-15N]		1.0 ~ 2.0 mM
2	ZINC (II) ION			51 mM

Sample #2

Temperature 310 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	translation initiation factor 2 beta	[U-15N]		1.0 ~ 2.0 mM
4	ZINC (II) ION			51 mM

2 1H-15N TOCSY

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Varian UNITYplus (800 MHz)

Sample #1

Temperature 310 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	translation initiation factor 2 beta	[U-13C; U-15N]		1.0 ~ 2.0 mM
2	ZINC (II) ION			51 mM

Sample #2

Temperature 310 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	translation initiation factor 2 beta	[U-15N]		1.0 ~ 2.0 mM
4	ZINC (II) ION			51 mM

3 HNCA

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Varian UNITYplus (800 MHz)

Sample #1

Temperature 310 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	translation initiation factor 2 beta	[U-13C; U-15N]		1.0 ~ 2.0 mM
2	ZINC (II) ION			51 mM

Sample #2

Temperature 310 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	translation initiation factor 2 beta	[U-15N]		1.0 ~ 2.0 mM
4	ZINC (II) ION			51 mM

4 HNCACB

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Varian UNITYplus (800 MHz)

Sample #1

Temperature 310 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	translation initiation factor 2 beta	[U-13C; U-15N]		1.0 ~ 2.0 mM
2	ZINC (II) ION			51 mM

Sample #2

Temperature 310 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	translation initiation factor 2 beta	[U-15N]		1.0 ~ 2.0 mM
4	ZINC (II) ION			51 mM

5 CBCA(CO)NH

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Varian UNITYplus (800 MHz)

Sample #1

Temperature 310 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	translation initiation factor 2 beta	[U-13C; U-15N]		1.0 ~ 2.0 mM
2	ZINC (II) ION			51 mM

Sample #2

Temperature 310 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	translation initiation factor 2 beta	[U-15N]		1.0 ~ 2.0 mM
4	ZINC (II) ION			51 mM

6 1H-13C NOESY

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Varian UNITYplus (800 MHz)

Sample #1

Temperature 310 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	translation initiation factor 2 beta	[U-13C; U-15N]		1.0 ~ 2.0 mM
2	ZINC (II) ION			51 mM

Sample #2

Temperature 310 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	translation initiation factor 2 beta	[U-15N]		1.0 ~ 2.0 mM
4	ZINC (II) ION			51 mM

nullKeywords aIF2beta, Zinc Finger, Translation initiation

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Found 1 Document (1.83 seconds)

BMRB: 5312

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Related entities 1. translation initiation factor 2 beta, : 1 : 1 : 119 entities Detail

Experiments performed 6 experiments Detail

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