BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	96.1 % (1266 of 1317)	96.2 % (662 of 688)	97.0 % (491 of 506)	91.9 % (113 of 123)
Backbone	96.5 % (641 of 664)	96.4 % (216 of 224)	97.3 % (323 of 332)	94.4 % (102 of 108)
Sidechain	95.9 % (730 of 761)	96.1 % (446 of 464)	96.8 % (273 of 282)	73.3 % (11 of 15)
Aromatic	100.0 % (60 of 60)	100.0 % (30 of 30)	100.0 % (29 of 29)	100.0 % (1 of 1)
Methyl	100.0 % (138 of 138)	100.0 % (69 of 69)	100.0 % (69 of 69)

1. Protein Yfia

TMNITSKQME ITPAIRQHVA DRLAKLEKWQ THLINPHIIL SKEPQGFVAD ATINTPNGVL VASGKHEDMY TAINELINKL ERQLNKLQHK GEARRAATSV KDANFVEEVE EE

Show sample condition

Sample

Temperature 304.5 (±1) K, pH 5.7 (±0.1)

#	Name	Isotope labeling	Concentration
6	Protein Yfia	[U-99% 13C; U-99% 15N]	1.5 mM
7	sodium phosphate		10 mM
8	NACL		50 mM
9	D2O		7 %
10	H2O		97 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.45 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.45 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.08 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.01 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1L4S, Strand ID: A Detail

Coupling constant

75 J values in 1 lists
Temperature 304.5 (±1) K, pH 5.7 (±0.1) Detail

Release date

2003-02-19

Citation

Ribosome-associated factor Y adopts a fold resembling a double-stranded RNA binding domain scaffold
Ye, K., Serganov, A., Hu, W., Patel, D.J.
Eur. J. Biochem. (2002), 269, 5182-5191, PubMed 12392550 , DOI: , Abstract

Related entities 1. Protein Yfia, : 1 : 23 : 4 : 24 entities Detail

Experiments performed 16 experiments Detail

1 2D 1H-1H NOESY

Instrument

Model Varian UnityPlus (600 MHz)

Sample #1

Temperature 304.5 (±1) K, pH 5.7 (±0.1)

#	Name	Isotope labeling	Concentration
1	Protein Yfia	[U-99% 15N]	2 mM
2	sodium phosphate		10 mM
3	NaCl		50 mM
4	D2O		7 %
5	H2O		93 %

Sample #2

Temperature 304.5 (±1) K, pH 5.7 (±0.1)

#	Name	Isotope labeling	Concentration
6	Protein Yfia	[U-99% 13C; U-99% 15N]	1.5 mM
7	sodium phosphate		10 mM
8	NACL		50 mM
9	D2O		7 %
10	H2O		97 %

2 2D 1H-1H TOCSY

Instrument

Model Varian UnityPlus (600 MHz)

Sample #1

Temperature 304.5 (±1) K, pH 5.7 (±0.1)

#	Name	Isotope labeling	Concentration
1	Protein Yfia	[U-99% 15N]	2 mM
2	sodium phosphate		10 mM
3	NaCl		50 mM
4	D2O		7 %
5	H2O		93 %

Sample #2

Temperature 304.5 (±1) K, pH 5.7 (±0.1)

#	Name	Isotope labeling	Concentration
6	Protein Yfia	[U-99% 13C; U-99% 15N]	1.5 mM
7	sodium phosphate		10 mM
8	NACL		50 mM
9	D2O		7 %
10	H2O		97 %

3 2D 1H-15N HSQC

Instrument

Model Varian UnityPlus (600 MHz)

Sample #1

Temperature 304.5 (±1) K, pH 5.7 (±0.1)

#	Name	Isotope labeling	Concentration
1	Protein Yfia	[U-99% 15N]	2 mM
2	sodium phosphate		10 mM
3	NaCl		50 mM
4	D2O		7 %
5	H2O		93 %

Sample #2

Temperature 304.5 (±1) K, pH 5.7 (±0.1)

#	Name	Isotope labeling	Concentration
6	Protein Yfia	[U-99% 13C; U-99% 15N]	1.5 mM
7	sodium phosphate		10 mM
8	NACL		50 mM
9	D2O		7 %
10	H2O		97 %

4 2D 1H-13C CT-HSQC

Instrument

Model Varian UnityPlus (600 MHz)

Sample #1

Temperature 304.5 (±1) K, pH 5.7 (±0.1)

#	Name	Isotope labeling	Concentration
1	Protein Yfia	[U-99% 15N]	2 mM
2	sodium phosphate		10 mM
3	NaCl		50 mM
4	D2O		7 %
5	H2O		93 %

Sample #2

Temperature 304.5 (±1) K, pH 5.7 (±0.1)

#	Name	Isotope labeling	Concentration
6	Protein Yfia	[U-99% 13C; U-99% 15N]	1.5 mM
7	sodium phosphate		10 mM
8	NACL		50 mM
9	D2O		7 %
10	H2O		97 %

5 3D CBCA(CO)NH

Instrument

Model Varian UnityPlus (600 MHz)

Sample #1

Temperature 304.5 (±1) K, pH 5.7 (±0.1)

#	Name	Isotope labeling	Concentration
1	Protein Yfia	[U-99% 15N]	2 mM
2	sodium phosphate		10 mM
3	NaCl		50 mM
4	D2O		7 %
5	H2O		93 %

Sample #2

Temperature 304.5 (±1) K, pH 5.7 (±0.1)

#	Name	Isotope labeling	Concentration
6	Protein Yfia	[U-99% 13C; U-99% 15N]	1.5 mM
7	sodium phosphate		10 mM
8	NACL		50 mM
9	D2O		7 %
10	H2O		97 %

6 3D HNCACB

Instrument

Model Varian UnityPlus (600 MHz)

Sample #1

Temperature 304.5 (±1) K, pH 5.7 (±0.1)

#	Name	Isotope labeling	Concentration
1	Protein Yfia	[U-99% 15N]	2 mM
2	sodium phosphate		10 mM
3	NaCl		50 mM
4	D2O		7 %
5	H2O		93 %

Sample #2

Temperature 304.5 (±1) K, pH 5.7 (±0.1)

#	Name	Isotope labeling	Concentration
6	Protein Yfia	[U-99% 13C; U-99% 15N]	1.5 mM
7	sodium phosphate		10 mM
8	NACL		50 mM
9	D2O		7 %
10	H2O		97 %

7 3D HNCO

Instrument

Model Varian UnityPlus (600 MHz)

Sample #1

Temperature 304.5 (±1) K, pH 5.7 (±0.1)

#	Name	Isotope labeling	Concentration
1	Protein Yfia	[U-99% 15N]	2 mM
2	sodium phosphate		10 mM
3	NaCl		50 mM
4	D2O		7 %
5	H2O		93 %

Sample #2

Temperature 304.5 (±1) K, pH 5.7 (±0.1)

#	Name	Isotope labeling	Concentration
6	Protein Yfia	[U-99% 13C; U-99% 15N]	1.5 mM
7	sodium phosphate		10 mM
8	NACL		50 mM
9	D2O		7 %
10	H2O		97 %

8 3D HN(CA)CO

Instrument

Model Varian UnityPlus (600 MHz)

Sample #1

Temperature 304.5 (±1) K, pH 5.7 (±0.1)

#	Name	Isotope labeling	Concentration
1	Protein Yfia	[U-99% 15N]	2 mM
2	sodium phosphate		10 mM
3	NaCl		50 mM
4	D2O		7 %
5	H2O		93 %

Sample #2

Temperature 304.5 (±1) K, pH 5.7 (±0.1)

#	Name	Isotope labeling	Concentration
6	Protein Yfia	[U-99% 13C; U-99% 15N]	1.5 mM
7	sodium phosphate		10 mM
8	NACL		50 mM
9	D2O		7 %
10	H2O		97 %

9 3D 1H-13C-1H HCCH-TOCSY

Instrument

Model Varian UnityPlus (600 MHz)

Sample #1

Temperature 304.5 (±1) K, pH 5.7 (±0.1)

#	Name	Isotope labeling	Concentration
1	Protein Yfia	[U-99% 15N]	2 mM
2	sodium phosphate		10 mM
3	NaCl		50 mM
4	D2O		7 %
5	H2O		93 %

Sample #2

Temperature 304.5 (±1) K, pH 5.7 (±0.1)

#	Name	Isotope labeling	Concentration
6	Protein Yfia	[U-99% 13C; U-99% 15N]	1.5 mM
7	sodium phosphate		10 mM
8	NACL		50 mM
9	D2O		7 %
10	H2O		97 %

10 3D 15N NOESY

Instrument

Model Varian UnityPlus (600 MHz)

Sample #1

Temperature 304.5 (±1) K, pH 5.7 (±0.1)

#	Name	Isotope labeling	Concentration
1	Protein Yfia	[U-99% 15N]	2 mM
2	sodium phosphate		10 mM
3	NaCl		50 mM
4	D2O		7 %
5	H2O		93 %

Sample #2

Temperature 304.5 (±1) K, pH 5.7 (±0.1)

#	Name	Isotope labeling	Concentration
6	Protein Yfia	[U-99% 13C; U-99% 15N]	1.5 mM
7	sodium phosphate		10 mM
8	NACL		50 mM
9	D2O		7 %
10	H2O		97 %

11 3D 15N TOCSY

Instrument

Model Varian UnityPlus (600 MHz)

Sample #1

Temperature 304.5 (±1) K, pH 5.7 (±0.1)

#	Name	Isotope labeling	Concentration
1	Protein Yfia	[U-99% 15N]	2 mM
2	sodium phosphate		10 mM
3	NaCl		50 mM
4	D2O		7 %
5	H2O		93 %

Sample #2

Temperature 304.5 (±1) K, pH 5.7 (±0.1)

#	Name	Isotope labeling	Concentration
6	Protein Yfia	[U-99% 13C; U-99% 15N]	1.5 mM
7	sodium phosphate		10 mM
8	NACL		50 mM
9	D2O		7 %
10	H2O		97 %

12 3D 13C NOESY

Instrument

Model Varian UnityPlus (600 MHz)

Sample #1

Temperature 304.5 (±1) K, pH 5.7 (±0.1)

#	Name	Isotope labeling	Concentration
1	Protein Yfia	[U-99% 15N]	2 mM
2	sodium phosphate		10 mM
3	NaCl		50 mM
4	D2O		7 %
5	H2O		93 %

Sample #2

Temperature 304.5 (±1) K, pH 5.7 (±0.1)

#	Name	Isotope labeling	Concentration
6	Protein Yfia	[U-99% 13C; U-99% 15N]	1.5 mM
7	sodium phosphate		10 mM
8	NACL		50 mM
9	D2O		7 %
10	H2O		97 %

13 3D H(CCO)NH

Instrument

Model Varian UnityPlus (600 MHz)

Sample #1

Temperature 304.5 (±1) K, pH 5.7 (±0.1)

#	Name	Isotope labeling	Concentration
1	Protein Yfia	[U-99% 15N]	2 mM
2	sodium phosphate		10 mM
3	NaCl		50 mM
4	D2O		7 %
5	H2O		93 %

Sample #2

Temperature 304.5 (±1) K, pH 5.7 (±0.1)

#	Name	Isotope labeling	Concentration
6	Protein Yfia	[U-99% 13C; U-99% 15N]	1.5 mM
7	sodium phosphate		10 mM
8	NACL		50 mM
9	D2O		7 %
10	H2O		97 %

14 3D C(CO)NH

Instrument

Model Varian UnityPlus (600 MHz)

Sample #1

Temperature 304.5 (±1) K, pH 5.7 (±0.1)

#	Name	Isotope labeling	Concentration
1	Protein Yfia	[U-99% 15N]	2 mM
2	sodium phosphate		10 mM
3	NaCl		50 mM
4	D2O		7 %
5	H2O		93 %

Sample #2

Temperature 304.5 (±1) K, pH 5.7 (±0.1)

#	Name	Isotope labeling	Concentration
6	Protein Yfia	[U-99% 13C; U-99% 15N]	1.5 mM
7	sodium phosphate		10 mM
8	NACL		50 mM
9	D2O		7 %
10	H2O		97 %

15 3D HNHA

Instrument

Model Varian UnityPlus (600 MHz)

Sample #1

Temperature 304.5 (±1) K, pH 5.7 (±0.1)

#	Name	Isotope labeling	Concentration
1	Protein Yfia	[U-99% 15N]	2 mM
2	sodium phosphate		10 mM
3	NaCl		50 mM
4	D2O		7 %
5	H2O		93 %

Sample #2

Temperature 304.5 (±1) K, pH 5.7 (±0.1)

#	Name	Isotope labeling	Concentration
6	Protein Yfia	[U-99% 13C; U-99% 15N]	1.5 mM
7	sodium phosphate		10 mM
8	NACL		50 mM
9	D2O		7 %
10	H2O		97 %

16 3D HNHB

Instrument

Model Varian UnityPlus (600 MHz)

Sample #1

Temperature 304.5 (±1) K, pH 5.7 (±0.1)

#	Name	Isotope labeling	Concentration
1	Protein Yfia	[U-99% 15N]	2 mM
2	sodium phosphate		10 mM
3	NaCl		50 mM
4	D2O		7 %
5	H2O		93 %

Sample #2

Temperature 304.5 (±1) K, pH 5.7 (±0.1)

#	Name	Isotope labeling	Concentration
6	Protein Yfia	[U-99% 13C; U-99% 15N]	1.5 mM
7	sodium phosphate		10 mM
8	NACL		50 mM
9	D2O		7 %
10	H2O		97 %

null

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BMRB: 5315

Sample

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. Protein Yfia, : 1 : 23 : 4 : 24 entities Detail

Experiments performed 16 experiments Detail

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