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1H and 15N Chemical Shift Assignments for Human B-FABP
Authors
Rademacher, M., Zimmerman, A.W., Rueterjans, H., Veerkamp, J.H., Luecke, C.
Assembly
B-FABP
Entity
1. B-FABP (polymer, Thiol state: all free), 131 monomers, 14757.54 Da Detail

VEAFCATWKL TNSQNFDEYM KALGVGFATR QVGNVTKPTV IISQEGDKVV IRTLSTFKNT EISFQLGEEF DETTADDRNC KSVVSLDGDK LVHIQKWDGK ETNFVREIKD GKMVMTLTFG DVVAVRHYEK A


Formula weight
14757.54 Da
Source organism
Homo sapiens
Exptl. method
NMR
Refine. method
TORSION ANGLE DYNAMICS COMBINED WITH SIMULATED ANNEALING FOLLOWED BY ENERGY MINIMIZATION
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 95.9 %, Completeness (bb): 97.3 % Detail

Polymer type: polypeptide(L)

Total1H15N
All95.9 % (892 of 930)95.6 % (752 of 787)97.9 % (140 of 143)
Backbone97.3 % (389 of 400)97.0 % (262 of 270)97.7 % (127 of 130)
Sidechain94.9 % (503 of 530)94.8 % (490 of 517)100.0 % (13 of 13)
Aromatic100.0 % (66 of 66)100.0 % (64 of 64)100.0 % (2 of 2)
Methyl98.7 % (75 of 76)98.7 % (75 of 76)

1. brain-type fatty acid binding protein

VEAFCATWKL TNSQNFDEYM KALGVGFATR QVGNVTKPTV IISQEGDKVV IRTLSTFKNT EISFQLGEEF DETTADDRNC KSVVSLDGDK LVHIQKWDGK ETNFVREIKD GKMVMTLTFG DVVAVRHYEK A

Sample #1

Temperature 298 (±0.1) K, pH 7.0 (±0.1)


#NameIsotope labelingTypeConcentration
1brain-type fatty acid binding protein2.0 ~ 4.0 mM
Sample #2

Temperature 298 (±0.1) K, pH 7.0 (±0.1)


#NameIsotope labelingTypeConcentration
2brain-type fatty acid binding protein[U-15N]2.0 ~ 4.0 mM

Protein Blocks Logo
Calculated from 20 models in PDB: 1JJX, Strand ID: A Detail


Release date
2002-11-03
Citation
Solution structure of fatty acid-binding protein from human brain
Rademacher, M., Zimmerman, A.W., Rueterjans, H., Veerkamp, J.H., Luecke, C.
Mol. Cell. Biochem. (2002), 239, 61-68, PubMed 12479569 , DOI: ,
Related entities 1. B-FABP, : 1 : 9 : 257 entities Detail
Experiments performed 6 experiments Detail
nullKeywords lipid binding protein, fatty acid carrier, holo form