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BMRB: 5321

1L1W

NMR structure of a SRP19 binding domain in human SRP RNA

Authors

Tabata, K., Sakamoto, T., Morita, S., Nakamura, K., Kawai, G.

Assembly

helix 6 of signal recognition particle RNA

Entity

1. helix 6 of signal recognition particle RNA (polymer, Thiol state: not present), 29 monomers, 9470.661 Da Detail

GGUGACCUCC CGGGAGCGGG GGACCACCA

Formula weight

9470.661 Da

Source organism

Homo sapiens

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 64.2 %, Completeness (bb): 60.9 % Detail

Polymer type: polyribonucleotide

	Total	¹H
All	64.2 % (163 of 254)	64.2 % (163 of 254)
Suger, PO4	60.9 % (106 of 174)	60.9 % (106 of 174)
Nucleobase	71.2 % (57 of 80)	71.2 % (57 of 80)
Aromatic	95.0 % (57 of 60)	95.0 % (57 of 60)

1. helix 6 of signal recognition particle RNA

GGUGACCUCC CGGGAGCGGG GGACCACCA

Show sample condition

Sample

Pressure 1 atm, Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Concentration
1	helix 6 of signal recognition particle RNA	0.5 mM
2	sodium phosphate	20 mM
3	NaCl	50 mM

Release date

2002-05-19

Citation

Solution structure of a SRP19 binding domain in human SRP RNA
Sakamoto, T., Morita, S., Tabata, K., Nakamura, K., Kawai, G.
J. Biochem. (2002), 132, 177-182, PubMed 12153712 , DOI: , Abstract

Related entities 1. helix 6 of signal recognition particle RNA, : 1 : 8 : 2 : 1 entities Detail

Experiments performed 8 experiments Detail

1 2D NOESY

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Varian UnityPlus (800 MHz)

Sample #1

Pressure 1 atm, Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Concentration
1	helix 6 of signal recognition particle RNA	0.5 mM
2	sodium phosphate	20 mM
3	NaCl	50 mM

Sample #2

Pressure 1 atm, Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
4	helix 6 of signal recognition particle RNA	[U-15N; U-13C]-Guanosine,Uridine	0.5 mM
5	sodium phosphate		20 mM
6	NaCl		50 mM

Sample #3

Pressure 1 atm, Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
7	helix 6 of signal recognition particle RNA	[U-15N; U-13C]-Adenosine,Cytidine	0.5 mM
8	sodium phosphate		20 mM
9	NaCl		50 mM

Sample #4

Pressure 1 atm, Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
10	helix 6 of signal recognition particle RNA	[U-15N; U-13C]-Guanosine,Cytidine	0.5 mM
11	sodium phosphate		20 mM
12	NaCl		50 mM

2 2D HNN-COSY

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Varian UnityPlus (800 MHz)

Sample #1

Pressure 1 atm, Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Concentration
1	helix 6 of signal recognition particle RNA	0.5 mM
2	sodium phosphate	20 mM
3	NaCl	50 mM

Sample #2

Pressure 1 atm, Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
4	helix 6 of signal recognition particle RNA	[U-15N; U-13C]-Guanosine,Uridine	0.5 mM
5	sodium phosphate		20 mM
6	NaCl		50 mM

Sample #3

Pressure 1 atm, Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
7	helix 6 of signal recognition particle RNA	[U-15N; U-13C]-Adenosine,Cytidine	0.5 mM
8	sodium phosphate		20 mM
9	NaCl		50 mM

Sample #4

Pressure 1 atm, Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
10	helix 6 of signal recognition particle RNA	[U-15N; U-13C]-Guanosine,Cytidine	0.5 mM
11	sodium phosphate		20 mM
12	NaCl		50 mM

3 2D half filtered NOESY

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Varian UnityPlus (800 MHz)

Sample #1

Pressure 1 atm, Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Concentration
1	helix 6 of signal recognition particle RNA	0.5 mM
2	sodium phosphate	20 mM
3	NaCl	50 mM

Sample #2

Pressure 1 atm, Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
4	helix 6 of signal recognition particle RNA	[U-15N; U-13C]-Guanosine,Uridine	0.5 mM
5	sodium phosphate		20 mM
6	NaCl		50 mM

Sample #3

Pressure 1 atm, Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
7	helix 6 of signal recognition particle RNA	[U-15N; U-13C]-Adenosine,Cytidine	0.5 mM
8	sodium phosphate		20 mM
9	NaCl		50 mM

Sample #4

Pressure 1 atm, Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
10	helix 6 of signal recognition particle RNA	[U-15N; U-13C]-Guanosine,Cytidine	0.5 mM
11	sodium phosphate		20 mM
12	NaCl		50 mM

4 DQF-COSY

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Varian UnityPlus (800 MHz)

Sample #1

Pressure 1 atm, Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Concentration
1	helix 6 of signal recognition particle RNA	0.5 mM
2	sodium phosphate	20 mM
3	NaCl	50 mM

Sample #2

Pressure 1 atm, Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
4	helix 6 of signal recognition particle RNA	[U-15N; U-13C]-Guanosine,Uridine	0.5 mM
5	sodium phosphate		20 mM
6	NaCl		50 mM

Sample #3

Pressure 1 atm, Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
7	helix 6 of signal recognition particle RNA	[U-15N; U-13C]-Adenosine,Cytidine	0.5 mM
8	sodium phosphate		20 mM
9	NaCl		50 mM

Sample #4

Pressure 1 atm, Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
10	helix 6 of signal recognition particle RNA	[U-15N; U-13C]-Guanosine,Cytidine	0.5 mM
11	sodium phosphate		20 mM
12	NaCl		50 mM

5 2D HP-COSY

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Varian UnityPlus (800 MHz)

Sample #1

Pressure 1 atm, Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Concentration
1	helix 6 of signal recognition particle RNA	0.5 mM
2	sodium phosphate	20 mM
3	NaCl	50 mM

Sample #2

Pressure 1 atm, Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
4	helix 6 of signal recognition particle RNA	[U-15N; U-13C]-Guanosine,Uridine	0.5 mM
5	sodium phosphate		20 mM
6	NaCl		50 mM

Sample #3

Pressure 1 atm, Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
7	helix 6 of signal recognition particle RNA	[U-15N; U-13C]-Adenosine,Cytidine	0.5 mM
8	sodium phosphate		20 mM
9	NaCl		50 mM

Sample #4

Pressure 1 atm, Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
10	helix 6 of signal recognition particle RNA	[U-15N; U-13C]-Guanosine,Cytidine	0.5 mM
11	sodium phosphate		20 mM
12	NaCl		50 mM

6 2D TOCSY

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Varian UnityPlus (800 MHz)

Sample #1

Pressure 1 atm, Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Concentration
1	helix 6 of signal recognition particle RNA	0.5 mM
2	sodium phosphate	20 mM
3	NaCl	50 mM

Sample #2

Pressure 1 atm, Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
4	helix 6 of signal recognition particle RNA	[U-15N; U-13C]-Guanosine,Uridine	0.5 mM
5	sodium phosphate		20 mM
6	NaCl		50 mM

Sample #3

Pressure 1 atm, Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
7	helix 6 of signal recognition particle RNA	[U-15N; U-13C]-Adenosine,Cytidine	0.5 mM
8	sodium phosphate		20 mM
9	NaCl		50 mM

Sample #4

Pressure 1 atm, Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
10	helix 6 of signal recognition particle RNA	[U-15N; U-13C]-Guanosine,Cytidine	0.5 mM
11	sodium phosphate		20 mM
12	NaCl		50 mM

7 2D HCCH-COSY

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Varian UnityPlus (800 MHz)

Sample #1

Pressure 1 atm, Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Concentration
1	helix 6 of signal recognition particle RNA	0.5 mM
2	sodium phosphate	20 mM
3	NaCl	50 mM

Sample #2

Pressure 1 atm, Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
4	helix 6 of signal recognition particle RNA	[U-15N; U-13C]-Guanosine,Uridine	0.5 mM
5	sodium phosphate		20 mM
6	NaCl		50 mM

Sample #3

Pressure 1 atm, Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
7	helix 6 of signal recognition particle RNA	[U-15N; U-13C]-Adenosine,Cytidine	0.5 mM
8	sodium phosphate		20 mM
9	NaCl		50 mM

Sample #4

Pressure 1 atm, Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
10	helix 6 of signal recognition particle RNA	[U-15N; U-13C]-Guanosine,Cytidine	0.5 mM
11	sodium phosphate		20 mM
12	NaCl		50 mM

8 2D HCCH-TOCSY

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Varian UnityPlus (800 MHz)

Sample #1

Pressure 1 atm, Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Concentration
1	helix 6 of signal recognition particle RNA	0.5 mM
2	sodium phosphate	20 mM
3	NaCl	50 mM

Sample #2

Pressure 1 atm, Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
4	helix 6 of signal recognition particle RNA	[U-15N; U-13C]-Guanosine,Uridine	0.5 mM
5	sodium phosphate		20 mM
6	NaCl		50 mM

Sample #3

Pressure 1 atm, Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
7	helix 6 of signal recognition particle RNA	[U-15N; U-13C]-Adenosine,Cytidine	0.5 mM
8	sodium phosphate		20 mM
9	NaCl		50 mM

Sample #4

Pressure 1 atm, Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Concentration
10	helix 6 of signal recognition particle RNA	[U-15N; U-13C]-Guanosine,Cytidine	0.5 mM
11	sodium phosphate		20 mM
12	NaCl		50 mM

nullKeywords GNAR motif, GNRA tetraloop, helix 6, NMR, RNA structure, signal recognition particle, tetraloop

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Found 1 Document (2.815 seconds)

BMRB: 5321

Sample

Related entities 1. helix 6 of signal recognition particle RNA, : 1 : 8 : 2 : 1 entities Detail

Experiments performed 8 experiments Detail

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