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Found 1 Document (1.818 seconds)

BMRB: 5323

1L1I

Solution Structure of the Tenebrio molitor Antifreeze Protein

Authors

Daley, M.E., Spyracopoulos, L., Jia, Z., Davies, P.L., Sykes, B.D.

Assembly

Thermal hysteresis protein isoform YL-1 (2-14)

Entity

1. Thermal hysteresis protein isoform YL-1 (2-14) (polymer, Thiol state: all disulfide bound), 84 monomers, 8384.048 Da Detail

QCTGGADCTS CTGACTGCGN CPNAVTCTNS QHCVKANTCT GSTDCNTAQT CTNSKDCFEA NTCTDSTNCY KATACTNSSG CPGH

Formula weight

8384.048 Da

Entity Connection

disulfide 8 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS2:SG	1:CYS11:SG
2	disulfide	sing	1:CYS8:SG	1:CYS18:SG
3	disulfide	sing	1:CYS15:SG	1:CYS21:SG
4	disulfide	sing	1:CYS27:SG	1:CYS33:SG
5	disulfide	sing	1:CYS39:SG	1:CYS45:SG
6	disulfide	sing	1:CYS51:SG	1:CYS57:SG
7	disulfide	sing	1:CYS63:SG	1:CYS69:SG
8	disulfide	sing	1:CYS75:SG	1:CYS81:SG

Source organism

Tenebrio molitor

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.6 %, Completeness: 92.2 %, Completeness (bb): 96.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹⁵N
All	92.2 % (447 of 485)	92.6 % (362 of 391)	90.4 % (85 of 94)
Backbone	96.9 % (248 of 256)	97.1 % (169 of 174)	96.3 % (79 of 82)
Sidechain	86.9 % (199 of 229)	88.9 % (193 of 217)	50.0 % (6 of 12)
Aromatic	69.2 % (9 of 13)	69.2 % (9 of 13)
Methyl	100.0 % (29 of 29)	100.0 % (29 of 29)

1. Tenebrio molitor antifreeze protein

QCTGGADCTS CTGACTGCGN CPNAVTCTNS QHCVKANTCT GSTDCNTAQT CTNSKDCFEA NTCTDSTNCY KATACTNSSG CPGH

Show sample condition

Sample

Pressure 1 atm, Temperature 303 (±0.1) K, pH 5.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	Tenebrio molitor antifreeze protein	[U-15N]	0.4 mM
2	H2O		90 %
3	D2O		10 %

Protein Blocks Logo

Calculated from 20 models in PDB: 1L1I, Strand ID: A Detail

Release date

2002-03-17

Citation

Structure and dynamics of a beta-helical antifreeze protein
Daley, M.E., Spyracopoulos, L., Jia, Z., Davies, P.L., Sykes, B.D.
Biochemistry (2002), 41, 5515-5525, PubMed 11969412 , DOI 10.1021/bi0121252 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Swiss-Prot: O16119 released on 2014-04-16
Title ANPY1_TENMO Entity Thermal hysteresis protein YL-1

Related entities 1. Thermal hysteresis protein isoform YL-1 (2-14), : 1 : 2 : 18 entities Detail

Experiments performed 8 experiments Detail

1 3D 15N-edited TOCSY

Instrument

#1 Model Varian Unity (600 MHz)

#2 Model Varian INOVA (800 MHz)

Sample #1

Pressure 1 atm, Temperature 303 (±0.1) K, pH 5.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	Tenebrio molitor antifreeze protein	[U-15N]	0.4 mM
2	H2O		90 %
3	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 303 (±0.1) K, pH 5.5 (±0.1)

#	Name	Concentration
4	Tenebrio molitor antifreeze protein	1.0 mM
5	H2O	90 %
6	D2O	10 %

Sample #3

Pressure 1 atm, Temperature 303 (±0.1) K, pH 5.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
7	Tenebrio molitor antifreeze protein			1.0 mM
8	D2O			100 %

2 HNHA

Instrument

#1 Model Varian Unity (600 MHz)

#2 Model Varian INOVA (800 MHz)

Sample #1

Pressure 1 atm, Temperature 303 (±0.1) K, pH 5.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	Tenebrio molitor antifreeze protein	[U-15N]	0.4 mM
2	H2O		90 %
3	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 303 (±0.1) K, pH 5.5 (±0.1)

#	Name	Concentration
4	Tenebrio molitor antifreeze protein	1.0 mM
5	H2O	90 %
6	D2O	10 %

Sample #3

Pressure 1 atm, Temperature 303 (±0.1) K, pH 5.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
7	Tenebrio molitor antifreeze protein			1.0 mM
8	D2O			100 %

3 HNHB

Instrument

#1 Model Varian Unity (600 MHz)

#2 Model Varian INOVA (800 MHz)

Sample #1

Pressure 1 atm, Temperature 303 (±0.1) K, pH 5.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	Tenebrio molitor antifreeze protein	[U-15N]	0.4 mM
2	H2O		90 %
3	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 303 (±0.1) K, pH 5.5 (±0.1)

#	Name	Concentration
4	Tenebrio molitor antifreeze protein	1.0 mM
5	H2O	90 %
6	D2O	10 %

Sample #3

Pressure 1 atm, Temperature 303 (±0.1) K, pH 5.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
7	Tenebrio molitor antifreeze protein			1.0 mM
8	D2O			100 %

4 3D 15N-edited NOESY

Instrument

#1 Model Varian Unity (600 MHz)

#2 Model Varian INOVA (800 MHz)

Sample #1

Pressure 1 atm, Temperature 303 (±0.1) K, pH 5.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	Tenebrio molitor antifreeze protein	[U-15N]	0.4 mM
2	H2O		90 %
3	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 303 (±0.1) K, pH 5.5 (±0.1)

#	Name	Concentration
4	Tenebrio molitor antifreeze protein	1.0 mM
5	H2O	90 %
6	D2O	10 %

Sample #3

Pressure 1 atm, Temperature 303 (±0.1) K, pH 5.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
7	Tenebrio molitor antifreeze protein			1.0 mM
8	D2O			100 %

5 2D TOCSY

Instrument

#1 Model Varian Unity (600 MHz)

#2 Model Varian INOVA (800 MHz)

Sample #1

Pressure 1 atm, Temperature 303 (±0.1) K, pH 5.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	Tenebrio molitor antifreeze protein	[U-15N]	0.4 mM
2	H2O		90 %
3	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 303 (±0.1) K, pH 5.5 (±0.1)

#	Name	Concentration
4	Tenebrio molitor antifreeze protein	1.0 mM
5	H2O	90 %
6	D2O	10 %

Sample #3

Pressure 1 atm, Temperature 303 (±0.1) K, pH 5.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
7	Tenebrio molitor antifreeze protein			1.0 mM
8	D2O			100 %

6 2D NOESY

Instrument

#1 Model Varian Unity (600 MHz)

#2 Model Varian INOVA (800 MHz)

Sample #1

Pressure 1 atm, Temperature 303 (±0.1) K, pH 5.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	Tenebrio molitor antifreeze protein	[U-15N]	0.4 mM
2	H2O		90 %
3	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 303 (±0.1) K, pH 5.5 (±0.1)

#	Name	Concentration
4	Tenebrio molitor antifreeze protein	1.0 mM
5	H2O	90 %
6	D2O	10 %

Sample #3

Pressure 1 atm, Temperature 303 (±0.1) K, pH 5.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
7	Tenebrio molitor antifreeze protein			1.0 mM
8	D2O			100 %

7 13C-HSQC

Instrument

#1 Model Varian Unity (600 MHz)

#2 Model Varian INOVA (800 MHz)

Sample #1

Pressure 1 atm, Temperature 303 (±0.1) K, pH 5.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	Tenebrio molitor antifreeze protein	[U-15N]	0.4 mM
2	H2O		90 %
3	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 303 (±0.1) K, pH 5.5 (±0.1)

#	Name	Concentration
4	Tenebrio molitor antifreeze protein	1.0 mM
5	H2O	90 %
6	D2O	10 %

Sample #3

Pressure 1 atm, Temperature 303 (±0.1) K, pH 5.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
7	Tenebrio molitor antifreeze protein			1.0 mM
8	D2O			100 %

8 15N-HSQC

Instrument

#1 Model Varian Unity (600 MHz)

#2 Model Varian INOVA (800 MHz)

Sample #1

Pressure 1 atm, Temperature 303 (±0.1) K, pH 5.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	Tenebrio molitor antifreeze protein	[U-15N]	0.4 mM
2	H2O		90 %
3	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 303 (±0.1) K, pH 5.5 (±0.1)

#	Name	Concentration
4	Tenebrio molitor antifreeze protein	1.0 mM
5	H2O	90 %
6	D2O	10 %

Sample #3

Pressure 1 atm, Temperature 303 (±0.1) K, pH 5.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
7	Tenebrio molitor antifreeze protein			1.0 mM
8	D2O			100 %

nullKeywords beta-helix

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Found 1 Document (1.818 seconds)

BMRB: 5323

Sample

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. Thermal hysteresis protein isoform YL-1 (2-14), : 1 : 2 : 18 entities Detail

Experiments performed 8 experiments Detail

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