BMRBj - BMREntryMaster

Found 1 Document (3.697 seconds)

BMRB: 5327

1L8C

Structural basis for Hif-1alpha/CBP recognition in the cellular hypoxic response

Authors

Dames, S., Martinez-Yamout, M., De Guzman, R.N., Dyson, H., Wright, P.E.

Assembly

Hif-1alpha/CBP complex

Entity

1. hypoxia induced factor 1 alpha (polymer, Thiol state: all free), 51 monomers, 5562.143 Da Detail

SDLACRLLGQ SMDESGLPQL TSYDCEVNAP IQGSRNLLQG EELLRALDQV N

2. CREB-binding protein (polymer, Thiol state: all other bound), 95 monomers, 10829.53 Da Detail

ADPEKRKLIQ QQLVLLLHAH KCQRREQANG EVRACSLPHC RTMKNVLNHM THCQAGKACQ VAHCASSRQI ISHWKNCTRH DCPVCLPLKN ASDKR

3. ZN (non-polymer), 65.409 × 3 Da

Total weight

16587.9 Da

Max. entity weight

10829.53 Da

Entity Connection

disulfide 9 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	2:CYS22:SG	3:ZN1:ZN
2	disulfide	sing	2:CYS35:SG	3:ZN1:ZN
3	disulfide	sing	2:CYS40:SG	3:ZN1:ZN
4	disulfide	sing	2:CYS53:SG	3:ZN1:ZN
5	disulfide	sing	2:CYS59:SG	3:ZN1:ZN
6	disulfide	sing	2:CYS64:SG	3:ZN1:ZN
7	disulfide	sing	2:CYS77:SG	3:ZN1:ZN
8	disulfide	sing	2:CYS82:SG	3:ZN1:ZN
9	disulfide	sing	2:CYS85:SG	3:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 53.4 %, Completeness: 45.1 %, Completeness (bb): 45.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	45.1 % (754 of 1670)	45.8 % (403 of 880)	42.7 % (268 of 628)	51.2 % (83 of 162)
Backbone	45.6 % (394 of 864)	51.0 % (149 of 292)	39.6 % (171 of 432)	52.9 % (74 of 140)
Sidechain	45.7 % (432 of 946)	43.2 % (254 of 588)	50.3 % (169 of 336)	40.9 % (9 of 22)
Aromatic	9.6 % (5 of 52)	11.5 % (3 of 26)	8.0 % (2 of 25)	0.0 % (0 of 1)
Methyl	63.8 % (97 of 152)	64.5 % (49 of 76)	63.2 % (48 of 76)

1. hypoxia induced factor 1 alpha

SDLACRLLGQ SMDESGLPQL TSYDCEVNAP IQGSRNLLQG EELLRALDQV N

2. CREB-binding protein

ADPEKRKLIQ QQLVLLLHAH KCQRREQANG EVRACSLPHC RTMKNVLNHM THCQAGKACQ VAHCASSRQI ISHWKNCTRH DCPVCLPLKN ASDKR

Show sample condition

Sample #1

Temperature 298 (±1) K, pH 6.1 (±0.2)

#	Name	Isotope labeling	Concentration
1	hypoxia induced factor 1 alpha		2 mM
2	CREB-binding protein	[U-13C; U-15N]	1 mM
3	ZINC (II) ION		3 mM
4	D-TRIS		10 mM
5	D-DTT		10 mM
6	NaN3		0.02 %
7	ZNSO4		0.5 mM
8	H2O		90 %
9	D2O		10 %

Sample #2

Temperature 298 (±1) K, pH 6.1 (±0.2)

#	Name	Isotope labeling	Concentration
10	hypoxia induced factor 1 alpha		2 mM
11	CREB-binding protein	[U-13C; U-15N]	1 mM
12	ZINC (II) ION		3 mM
13	D-TRIS		10 mM
14	D-DTT		10 mM
15	NaN3		0.02 %
16	ZNSO4		0.5 mM
17	D2O		100 %

Sample #3

Temperature 298 (±1) K, pH 6.1 (±0.2)

#	Name	Isotope labeling	Concentration
18	hypoxia induced factor 1 alpha		2 mM
19	CREB-binding protein	[U-15N]	1 mM
20	ZINC (II) ION		3 mM
21	D-TRIS		10 mM
22	D-DTT		10 mM
23	NaN3		0.02 %
24	ZNSO4		0.5 mM
25	H2O		90 %
26	D2O		10 %

Sample #4

Temperature 298 (±1) K, pH 6.1 (±0.2)

#	Name	Isotope labeling	Concentration
27	hypoxia induced factor 1 alpha	[U-13C; U-15N]	1 mM
28	CREB-binding protein		2 mM
29	ZINC (II) ION		6 mM
30	D-TRIS		10 mM
31	D-DTT		10 mM
32	NaN3		0.02 %
33	ZNSO4		0.5 mM
34	H2O		90 %
35	D2O		10 %

Sample #5

Temperature 298 (±1) K, pH 6.1 (±0.2)

#	Name	Isotope labeling	Concentration
36	hypoxia induced factor 1 alpha	[U-13C; U-15N]	1 mM
37	CREB-binding protein		2 mM
38	ZINC (II) ION		6 mM
39	D-TRIS		10 mM
40	D-DTT		10 mM
41	NaN3		0.02 %
42	ZNSO4		0.5 mM
43	D2O		100 %

Sample #6

Temperature 298 (±1) K, pH 6.1 (±0.2)

#	Name	Isotope labeling	Concentration
44	hypoxia induced factor 1 alpha	[U-15N]	1 mM
45	CREB-binding protein		2 mM
46	ZINC (II) ION		6 mM
47	D-TRIS		10 mM
48	D-DTT		10 mM
49	NaN3		0.02 %
50	ZNSO4		0.5 mM
51	H2O		90 %
52	D2O		10 %

Chem. Shift Complete2

Sequence coverage: 71.2 %, Completeness: 52.6 %, Completeness (bb): 51.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	52.6 % (1756 of 3340)	54.5 % (959 of 1760)	48.9 % (614 of 1256)	56.5 % (183 of 324)
Backbone	51.6 % (891 of 1728)	58.4 % (341 of 584)	45.0 % (389 of 864)	57.5 % (161 of 280)
Sidechain	54.6 % (1033 of 1892)	52.6 % (618 of 1176)	58.5 % (393 of 672)	50.0 % (22 of 44)
Aromatic	33.7 % (35 of 104)	34.6 % (18 of 52)	32.0 % (16 of 50)	50.0 % (1 of 2)
Methyl	64.1 % (195 of 304)	65.1 % (99 of 152)	63.2 % (96 of 152)

1. hypoxia induced factor 1 alpha

SDLACRLLGQ SMDESGLPQL TSYDCEVNAP IQGSRNLLQG EELLRALDQV N

2. CREB-binding protein

ADPEKRKLIQ QQLVLLLHAH KCQRREQANG EVRACSLPHC RTMKNVLNHM THCQAGKACQ VAHCASSRQI ISHWKNCTRH DCPVCLPLKN ASDKR

Show sample condition

Sample #1

Temperature 298 (±1) K, pH 6.1 (±0.2)

#	Name	Isotope labeling	Concentration
1	hypoxia induced factor 1 alpha		2 mM
2	CREB-binding protein	[U-13C; U-15N]	1 mM
3	ZINC (II) ION		3 mM
4	D-TRIS		10 mM
5	D-DTT		10 mM
6	NaN3		0.02 %
7	ZNSO4		0.5 mM
8	H2O		90 %
9	D2O		10 %

Sample #2

Temperature 298 (±1) K, pH 6.1 (±0.2)

#	Name	Isotope labeling	Concentration
10	hypoxia induced factor 1 alpha		2 mM
11	CREB-binding protein	[U-13C; U-15N]	1 mM
12	ZINC (II) ION		3 mM
13	D-TRIS		10 mM
14	D-DTT		10 mM
15	NaN3		0.02 %
16	ZNSO4		0.5 mM
17	D2O		100 %

Sample #3

Temperature 298 (±1) K, pH 6.1 (±0.2)

#	Name	Isotope labeling	Concentration
18	hypoxia induced factor 1 alpha		2 mM
19	CREB-binding protein	[U-15N]	1 mM
20	ZINC (II) ION		3 mM
21	D-TRIS		10 mM
22	D-DTT		10 mM
23	NaN3		0.02 %
24	ZNSO4		0.5 mM
25	H2O		90 %
26	D2O		10 %

Sample #4

Temperature 298 (±1) K, pH 6.1 (±0.2)

#	Name	Isotope labeling	Concentration
27	hypoxia induced factor 1 alpha	[U-13C; U-15N]	1 mM
28	CREB-binding protein		2 mM
29	ZINC (II) ION		6 mM
30	D-TRIS		10 mM
31	D-DTT		10 mM
32	NaN3		0.02 %
33	ZNSO4		0.5 mM
34	H2O		90 %
35	D2O		10 %

Sample #5

Temperature 298 (±1) K, pH 6.1 (±0.2)

#	Name	Isotope labeling	Concentration
36	hypoxia induced factor 1 alpha	[U-13C; U-15N]	1 mM
37	CREB-binding protein		2 mM
38	ZINC (II) ION		6 mM
39	D-TRIS		10 mM
40	D-DTT		10 mM
41	NaN3		0.02 %
42	ZNSO4		0.5 mM
43	D2O		100 %

Sample #6

Temperature 298 (±1) K, pH 6.1 (±0.2)

#	Name	Isotope labeling	Concentration
44	hypoxia induced factor 1 alpha	[U-15N]	1 mM
45	CREB-binding protein		2 mM
46	ZINC (II) ION		6 mM
47	D-TRIS		10 mM
48	D-DTT		10 mM
49	NaN3		0.02 %
50	ZNSO4		0.5 mM
51	H2O		90 %
52	D2O		10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.33 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.33 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.12 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1L8C, Strand ID: A, B Detail

Release date

2002-03-18

Citation

Structural basis for Hif-1 alpha /CBP recognition in the cellular hypoxic response
Dames, S.A., Martinez-Yamout, M., De Guzman, R.N., Dyson, H.J., Wright, P.E.
Proc. Natl. Acad. Sci. U. S. A. (2002), 99, 5271-5276, PubMed 11959977 , DOI 10.1073/pnas.082121399 , Abstract

Related entities 1. hypoxia induced factor 1 alpha, : 1 : 8 : 6 : 15 entities Detail

Related entities 2. CREB-binding protein, : 6 : 1 : 40 entities Detail

Interaction partners 1. hypoxia induced factor 1 alpha, : 67 : 1 interactors Detail

More details for 68 interactors identified by 41 citations

Interaction partners 2. CREB-binding protein, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 2 experiments Detail

1 1H-15N NOESY

Instrument

#1 Model Bruker DRX (800 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.1 (±0.2)

#	Name	Isotope labeling	Concentration
1	hypoxia induced factor 1 alpha		2 mM
2	CREB-binding protein	[U-13C; U-15N]	1 mM
3	ZINC (II) ION		3 mM
4	D-TRIS		10 mM
5	D-DTT		10 mM
6	NaN3		0.02 %
7	ZNSO4		0.5 mM
8	H2O		90 %
9	D2O		10 %

Sample #2

Temperature 298 (±1) K, pH 6.1 (±0.2)

#	Name	Isotope labeling	Concentration
10	hypoxia induced factor 1 alpha		2 mM
11	CREB-binding protein	[U-13C; U-15N]	1 mM
12	ZINC (II) ION		3 mM
13	D-TRIS		10 mM
14	D-DTT		10 mM
15	NaN3		0.02 %
16	ZNSO4		0.5 mM
17	D2O		100 %

Sample #3

Temperature 298 (±1) K, pH 6.1 (±0.2)

#	Name	Isotope labeling	Concentration
18	hypoxia induced factor 1 alpha		2 mM
19	CREB-binding protein	[U-15N]	1 mM
20	ZINC (II) ION		3 mM
21	D-TRIS		10 mM
22	D-DTT		10 mM
23	NaN3		0.02 %
24	ZNSO4		0.5 mM
25	H2O		90 %
26	D2O		10 %

Sample #4

Temperature 298 (±1) K, pH 6.1 (±0.2)

#	Name	Isotope labeling	Concentration
27	hypoxia induced factor 1 alpha	[U-13C; U-15N]	1 mM
28	CREB-binding protein		2 mM
29	ZINC (II) ION		6 mM
30	D-TRIS		10 mM
31	D-DTT		10 mM
32	NaN3		0.02 %
33	ZNSO4		0.5 mM
34	H2O		90 %
35	D2O		10 %

Sample #5

Temperature 298 (±1) K, pH 6.1 (±0.2)

#	Name	Isotope labeling	Concentration
36	hypoxia induced factor 1 alpha	[U-13C; U-15N]	1 mM
37	CREB-binding protein		2 mM
38	ZINC (II) ION		6 mM
39	D-TRIS		10 mM
40	D-DTT		10 mM
41	NaN3		0.02 %
42	ZNSO4		0.5 mM
43	D2O		100 %

Sample #6

Temperature 298 (±1) K, pH 6.1 (±0.2)

#	Name	Isotope labeling	Concentration
44	hypoxia induced factor 1 alpha	[U-15N]	1 mM
45	CREB-binding protein		2 mM
46	ZINC (II) ION		6 mM
47	D-TRIS		10 mM
48	D-DTT		10 mM
49	NaN3		0.02 %
50	ZNSO4		0.5 mM
51	H2O		90 %
52	D2O		10 %

2 1H-13C NOESY

Instrument

#1 Model Bruker DRX (800 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.1 (±0.2)

#	Name	Isotope labeling	Concentration
1	hypoxia induced factor 1 alpha		2 mM
2	CREB-binding protein	[U-13C; U-15N]	1 mM
3	ZINC (II) ION		3 mM
4	D-TRIS		10 mM
5	D-DTT		10 mM
6	NaN3		0.02 %
7	ZNSO4		0.5 mM
8	H2O		90 %
9	D2O		10 %

Sample #2

Temperature 298 (±1) K, pH 6.1 (±0.2)

#	Name	Isotope labeling	Concentration
10	hypoxia induced factor 1 alpha		2 mM
11	CREB-binding protein	[U-13C; U-15N]	1 mM
12	ZINC (II) ION		3 mM
13	D-TRIS		10 mM
14	D-DTT		10 mM
15	NaN3		0.02 %
16	ZNSO4		0.5 mM
17	D2O		100 %

Sample #3

Temperature 298 (±1) K, pH 6.1 (±0.2)

#	Name	Isotope labeling	Concentration
18	hypoxia induced factor 1 alpha		2 mM
19	CREB-binding protein	[U-15N]	1 mM
20	ZINC (II) ION		3 mM
21	D-TRIS		10 mM
22	D-DTT		10 mM
23	NaN3		0.02 %
24	ZNSO4		0.5 mM
25	H2O		90 %
26	D2O		10 %

Sample #4

Temperature 298 (±1) K, pH 6.1 (±0.2)

#	Name	Isotope labeling	Concentration
27	hypoxia induced factor 1 alpha	[U-13C; U-15N]	1 mM
28	CREB-binding protein		2 mM
29	ZINC (II) ION		6 mM
30	D-TRIS		10 mM
31	D-DTT		10 mM
32	NaN3		0.02 %
33	ZNSO4		0.5 mM
34	H2O		90 %
35	D2O		10 %

Sample #5

Temperature 298 (±1) K, pH 6.1 (±0.2)

#	Name	Isotope labeling	Concentration
36	hypoxia induced factor 1 alpha	[U-13C; U-15N]	1 mM
37	CREB-binding protein		2 mM
38	ZINC (II) ION		6 mM
39	D-TRIS		10 mM
40	D-DTT		10 mM
41	NaN3		0.02 %
42	ZNSO4		0.5 mM
43	D2O		100 %

Sample #6

Temperature 298 (±1) K, pH 6.1 (±0.2)

#	Name	Isotope labeling	Concentration
44	hypoxia induced factor 1 alpha	[U-15N]	1 mM
45	CREB-binding protein		2 mM
46	ZINC (II) ION		6 mM
47	D-TRIS		10 mM
48	D-DTT		10 mM
49	NaN3		0.02 %
50	ZNSO4		0.5 mM
51	H2O		90 %
52	D2O		10 %

nullKeywords CBP, HIF-1alpha, TAZ1, hypoxia

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Found 1 Document (3.697 seconds)

BMRB: 5327

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. hypoxia induced factor 1 alpha, : 1 : 8 : 6 : 15 entities Detail

Related entities 2. CREB-binding protein, : 6 : 1 : 40 entities Detail

Interaction partners 1. hypoxia induced factor 1 alpha, : 67 : 1 interactors Detail

Interaction partners 2. CREB-binding protein, : 1 interactors Detail

Experiments performed 2 experiments Detail

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