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BMRB: 5329

1L7Y

Backbone and Side Chain 1H, 13C, and 15N chemical shift assignments for conserved eukaryotic protein ZK652.3 from C. elegans

Authors

Cort, J.R., Chiang, Y., Deyou, Z., Montelione, G.T., Kennedy, M.A.

Assembly

C. elegans protein ZK652.3

Entity

1. C. elegans protein ZK652.3 (polymer, Thiol state: not present), 94 monomers, 9791.152 Da Detail

MSGGTAATTA GSKVTFKITL TSDPKLPFKV LSVPESTPFT AVLKFAAEEF KVPAATSAII TNDGVGVNPA QPAGNIFLKH GSELRLIPRD RVGH

Formula weight

9791.152 Da

Source organism

Caenorhabditis elegans

Exptl. method

NMR

Refine. method

distance geometry, simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.9 %, Completeness: 84.5 %, Completeness (bb): 91.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	84.5 % (885 of 1047)	81.6 % (438 of 537)	86.2 % (362 of 420)	94.4 % (85 of 90)
Backbone	91.8 % (503 of 548)	88.3 % (166 of 188)	93.4 % (256 of 274)	94.2 % (81 of 86)
Sidechain	77.9 % (456 of 585)	76.2 % (266 of 349)	80.2 % (186 of 232)	100.0 % (4 of 4)
Aromatic	41.2 % (28 of 68)	44.1 % (15 of 34)	38.2 % (13 of 34)
Methyl	82.0 % (100 of 122)	80.3 % (49 of 61)	83.6 % (51 of 61)

1. ZK652.3

MSGGTAATTA GSKVTFKITL TSDPKLPFKV LSVPESTPFT AVLKFAAEEF KVPAATSAII TNDGVGVNPA QPAGNIFLKH GSELRLIPRD RVGH

Show sample condition

Sample #1

Temperature 298 (±1) K, pH 5.5 (±0.05)

#	Name	Isotope labeling	Concentration
1	ZK652.3	[U-99% 15N]	0.9 mM
2	ammonium acetate		10 mM
3	sodium chloride		50 mM

Sample #2

Temperature 298 (±1) K, pH 5.5 (±0.05)

#	Name	Isotope labeling	Concentration
4	ZK652.3	[U-99% 13C; U-99% 15N]	0.9 mM
5	ammonium acetate		10 mM
6	sodium chloride		50 mM

Sample #3

Temperature 298 (±1) K, pH 5.5 (±0.05)

#	Name	Isotope labeling	Concentration
7	ZK652.3	[U-10% 13C; U-99% 15N]	0.9 mM
8	ammonium acetate		10 mM
9	sodium chloride		50 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.06 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.06 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.07 ppm, Outliers: 3 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.29 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 24 models in PDB: 1L7Y, Strand ID: A Detail

Release date

2003-01-05

Citation

NMR structure of conserved eukaryotic protein ZK652.3 from C. elegans: a ubiquitin-like fold
Cort, J.R., Chiang, Y., Zheng, D., Montelione, G.T., Kennedy, M.A.
Proteins (2002), 48, 733-736, PubMed 12211038 , DOI 10.1002/prot.10197 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Swiss-Prot: P34661 released on 1994-02-01
Title UFM1_CAEEL Entity Ubiquitin-fold modifier 1

Related entities 1. C. elegans protein ZK652.3, : 1 : 2 : 1 : 59 entities Detail

Interaction partners 1. C. elegans protein ZK652.3, : 2 interactors Detail

More details for 2 interactors identified by 2 citations

Experiments performed 16 experiments Detail

1 2D 1H-15N HSQC

Instrument

#1 Model Varian UNITY (600 MHz)

#2 Model Varian INOVA (600 MHz)

#3 Model Varian INOVA (750 MHz)

#4 Model Varian INOVA (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 5.5 (±0.05)

#	Name	Isotope labeling	Concentration
1	ZK652.3	[U-99% 15N]	0.9 mM
2	ammonium acetate		10 mM
3	sodium chloride		50 mM

Sample #2

Temperature 298 (±1) K, pH 5.5 (±0.05)

#	Name	Isotope labeling	Concentration
4	ZK652.3	[U-99% 13C; U-99% 15N]	0.9 mM
5	ammonium acetate		10 mM
6	sodium chloride		50 mM

Sample #3

Temperature 298 (±1) K, pH 5.5 (±0.05)

#	Name	Isotope labeling	Concentration
7	ZK652.3	[U-10% 13C; U-99% 15N]	0.9 mM
8	ammonium acetate		10 mM
9	sodium chloride		50 mM

2 3D 1H-15N NOESY-HSQC

Instrument

#1 Model Varian UNITY (600 MHz)

#2 Model Varian INOVA (600 MHz)

#3 Model Varian INOVA (750 MHz)

#4 Model Varian INOVA (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 5.5 (±0.05)

#	Name	Isotope labeling	Concentration
1	ZK652.3	[U-99% 15N]	0.9 mM
2	ammonium acetate		10 mM
3	sodium chloride		50 mM

Sample #2

Temperature 298 (±1) K, pH 5.5 (±0.05)

#	Name	Isotope labeling	Concentration
4	ZK652.3	[U-99% 13C; U-99% 15N]	0.9 mM
5	ammonium acetate		10 mM
6	sodium chloride		50 mM

Sample #3

Temperature 298 (±1) K, pH 5.5 (±0.05)

#	Name	Isotope labeling	Concentration
7	ZK652.3	[U-10% 13C; U-99% 15N]	0.9 mM
8	ammonium acetate		10 mM
9	sodium chloride		50 mM

3 3D HNHA

Instrument

#1 Model Varian UNITY (600 MHz)

#2 Model Varian INOVA (600 MHz)

#3 Model Varian INOVA (750 MHz)

#4 Model Varian INOVA (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 5.5 (±0.05)

#	Name	Isotope labeling	Concentration
1	ZK652.3	[U-99% 15N]	0.9 mM
2	ammonium acetate		10 mM
3	sodium chloride		50 mM

Sample #2

Temperature 298 (±1) K, pH 5.5 (±0.05)

#	Name	Isotope labeling	Concentration
4	ZK652.3	[U-99% 13C; U-99% 15N]	0.9 mM
5	ammonium acetate		10 mM
6	sodium chloride		50 mM

Sample #3

Temperature 298 (±1) K, pH 5.5 (±0.05)

#	Name	Isotope labeling	Concentration
7	ZK652.3	[U-10% 13C; U-99% 15N]	0.9 mM
8	ammonium acetate		10 mM
9	sodium chloride		50 mM

4 2D 1H-13C HSQC (aliph)

Instrument

#1 Model Varian UNITY (600 MHz)

#2 Model Varian INOVA (600 MHz)

#3 Model Varian INOVA (750 MHz)

#4 Model Varian INOVA (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 5.5 (±0.05)

#	Name	Isotope labeling	Concentration
1	ZK652.3	[U-99% 15N]	0.9 mM
2	ammonium acetate		10 mM
3	sodium chloride		50 mM

Sample #2

Temperature 298 (±1) K, pH 5.5 (±0.05)

#	Name	Isotope labeling	Concentration
4	ZK652.3	[U-99% 13C; U-99% 15N]	0.9 mM
5	ammonium acetate		10 mM
6	sodium chloride		50 mM

Sample #3

Temperature 298 (±1) K, pH 5.5 (±0.05)

#	Name	Isotope labeling	Concentration
7	ZK652.3	[U-10% 13C; U-99% 15N]	0.9 mM
8	ammonium acetate		10 mM
9	sodium chloride		50 mM

5 HNCACB

Instrument

#1 Model Varian UNITY (600 MHz)

#2 Model Varian INOVA (600 MHz)

#3 Model Varian INOVA (750 MHz)

#4 Model Varian INOVA (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 5.5 (±0.05)

#	Name	Isotope labeling	Concentration
1	ZK652.3	[U-99% 15N]	0.9 mM
2	ammonium acetate		10 mM
3	sodium chloride		50 mM

Sample #2

Temperature 298 (±1) K, pH 5.5 (±0.05)

#	Name	Isotope labeling	Concentration
4	ZK652.3	[U-99% 13C; U-99% 15N]	0.9 mM
5	ammonium acetate		10 mM
6	sodium chloride		50 mM

Sample #3

Temperature 298 (±1) K, pH 5.5 (±0.05)

#	Name	Isotope labeling	Concentration
7	ZK652.3	[U-10% 13C; U-99% 15N]	0.9 mM
8	ammonium acetate		10 mM
9	sodium chloride		50 mM

6 HNCO

Instrument

#1 Model Varian UNITY (600 MHz)

#2 Model Varian INOVA (600 MHz)

#3 Model Varian INOVA (750 MHz)

#4 Model Varian INOVA (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 5.5 (±0.05)

#	Name	Isotope labeling	Concentration
1	ZK652.3	[U-99% 15N]	0.9 mM
2	ammonium acetate		10 mM
3	sodium chloride		50 mM

Sample #2

Temperature 298 (±1) K, pH 5.5 (±0.05)

#	Name	Isotope labeling	Concentration
4	ZK652.3	[U-99% 13C; U-99% 15N]	0.9 mM
5	ammonium acetate		10 mM
6	sodium chloride		50 mM

Sample #3

Temperature 298 (±1) K, pH 5.5 (±0.05)

#	Name	Isotope labeling	Concentration
7	ZK652.3	[U-10% 13C; U-99% 15N]	0.9 mM
8	ammonium acetate		10 mM
9	sodium chloride		50 mM

7 CBCA(CO)NH

Instrument

#1 Model Varian UNITY (600 MHz)

#2 Model Varian INOVA (600 MHz)

#3 Model Varian INOVA (750 MHz)

#4 Model Varian INOVA (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 5.5 (±0.05)

#	Name	Isotope labeling	Concentration
1	ZK652.3	[U-99% 15N]	0.9 mM
2	ammonium acetate		10 mM
3	sodium chloride		50 mM

Sample #2

Temperature 298 (±1) K, pH 5.5 (±0.05)

#	Name	Isotope labeling	Concentration
4	ZK652.3	[U-99% 13C; U-99% 15N]	0.9 mM
5	ammonium acetate		10 mM
6	sodium chloride		50 mM

Sample #3

Temperature 298 (±1) K, pH 5.5 (±0.05)

#	Name	Isotope labeling	Concentration
7	ZK652.3	[U-10% 13C; U-99% 15N]	0.9 mM
8	ammonium acetate		10 mM
9	sodium chloride		50 mM

8 CBCACOCAHA

Instrument

#1 Model Varian UNITY (600 MHz)

#2 Model Varian INOVA (600 MHz)

#3 Model Varian INOVA (750 MHz)

#4 Model Varian INOVA (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 5.5 (±0.05)

#	Name	Isotope labeling	Concentration
1	ZK652.3	[U-99% 15N]	0.9 mM
2	ammonium acetate		10 mM
3	sodium chloride		50 mM

Sample #2

Temperature 298 (±1) K, pH 5.5 (±0.05)

#	Name	Isotope labeling	Concentration
4	ZK652.3	[U-99% 13C; U-99% 15N]	0.9 mM
5	ammonium acetate		10 mM
6	sodium chloride		50 mM

Sample #3

Temperature 298 (±1) K, pH 5.5 (±0.05)

#	Name	Isotope labeling	Concentration
7	ZK652.3	[U-10% 13C; U-99% 15N]	0.9 mM
8	ammonium acetate		10 mM
9	sodium chloride		50 mM

9 HCCH-TOCSY

Instrument

#1 Model Varian UNITY (600 MHz)

#2 Model Varian INOVA (600 MHz)

#3 Model Varian INOVA (750 MHz)

#4 Model Varian INOVA (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 5.5 (±0.05)

#	Name	Isotope labeling	Concentration
1	ZK652.3	[U-99% 15N]	0.9 mM
2	ammonium acetate		10 mM
3	sodium chloride		50 mM

Sample #2

Temperature 298 (±1) K, pH 5.5 (±0.05)

#	Name	Isotope labeling	Concentration
4	ZK652.3	[U-99% 13C; U-99% 15N]	0.9 mM
5	ammonium acetate		10 mM
6	sodium chloride		50 mM

Sample #3

Temperature 298 (±1) K, pH 5.5 (±0.05)

#	Name	Isotope labeling	Concentration
7	ZK652.3	[U-10% 13C; U-99% 15N]	0.9 mM
8	ammonium acetate		10 mM
9	sodium chloride		50 mM

10 HCC-TOCSY-NNH

Instrument

#1 Model Varian UNITY (600 MHz)

#2 Model Varian INOVA (600 MHz)

#3 Model Varian INOVA (750 MHz)

#4 Model Varian INOVA (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 5.5 (±0.05)

#	Name	Isotope labeling	Concentration
1	ZK652.3	[U-99% 15N]	0.9 mM
2	ammonium acetate		10 mM
3	sodium chloride		50 mM

Sample #2

Temperature 298 (±1) K, pH 5.5 (±0.05)

#	Name	Isotope labeling	Concentration
4	ZK652.3	[U-99% 13C; U-99% 15N]	0.9 mM
5	ammonium acetate		10 mM
6	sodium chloride		50 mM

Sample #3

Temperature 298 (±1) K, pH 5.5 (±0.05)

#	Name	Isotope labeling	Concentration
7	ZK652.3	[U-10% 13C; U-99% 15N]	0.9 mM
8	ammonium acetate		10 mM
9	sodium chloride		50 mM

11 CCC-TOCSY-NNH

Instrument

#1 Model Varian UNITY (600 MHz)

#2 Model Varian INOVA (600 MHz)

#3 Model Varian INOVA (750 MHz)

#4 Model Varian INOVA (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 5.5 (±0.05)

#	Name	Isotope labeling	Concentration
1	ZK652.3	[U-99% 15N]	0.9 mM
2	ammonium acetate		10 mM
3	sodium chloride		50 mM

Sample #2

Temperature 298 (±1) K, pH 5.5 (±0.05)

#	Name	Isotope labeling	Concentration
4	ZK652.3	[U-99% 13C; U-99% 15N]	0.9 mM
5	ammonium acetate		10 mM
6	sodium chloride		50 mM

Sample #3

Temperature 298 (±1) K, pH 5.5 (±0.05)

#	Name	Isotope labeling	Concentration
7	ZK652.3	[U-10% 13C; U-99% 15N]	0.9 mM
8	ammonium acetate		10 mM
9	sodium chloride		50 mM

12 3D CN-NOESY-HSQC

Instrument

#1 Model Varian UNITY (600 MHz)

#2 Model Varian INOVA (600 MHz)

#3 Model Varian INOVA (750 MHz)

#4 Model Varian INOVA (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 5.5 (±0.05)

#	Name	Isotope labeling	Concentration
1	ZK652.3	[U-99% 15N]	0.9 mM
2	ammonium acetate		10 mM
3	sodium chloride		50 mM

Sample #2

Temperature 298 (±1) K, pH 5.5 (±0.05)

#	Name	Isotope labeling	Concentration
4	ZK652.3	[U-99% 13C; U-99% 15N]	0.9 mM
5	ammonium acetate		10 mM
6	sodium chloride		50 mM

Sample #3

Temperature 298 (±1) K, pH 5.5 (±0.05)

#	Name	Isotope labeling	Concentration
7	ZK652.3	[U-10% 13C; U-99% 15N]	0.9 mM
8	ammonium acetate		10 mM
9	sodium chloride		50 mM

13 4D CC-NOESY

Instrument

#1 Model Varian UNITY (600 MHz)

#2 Model Varian INOVA (600 MHz)

#3 Model Varian INOVA (750 MHz)

#4 Model Varian INOVA (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 5.5 (±0.05)

#	Name	Isotope labeling	Concentration
1	ZK652.3	[U-99% 15N]	0.9 mM
2	ammonium acetate		10 mM
3	sodium chloride		50 mM

Sample #2

Temperature 298 (±1) K, pH 5.5 (±0.05)

#	Name	Isotope labeling	Concentration
4	ZK652.3	[U-99% 13C; U-99% 15N]	0.9 mM
5	ammonium acetate		10 mM
6	sodium chloride		50 mM

Sample #3

Temperature 298 (±1) K, pH 5.5 (±0.05)

#	Name	Isotope labeling	Concentration
7	ZK652.3	[U-10% 13C; U-99% 15N]	0.9 mM
8	ammonium acetate		10 mM
9	sodium chloride		50 mM

14 2D 1H-13C HSQC (alpha)

Instrument

#1 Model Varian UNITY (600 MHz)

#2 Model Varian INOVA (600 MHz)

#3 Model Varian INOVA (750 MHz)

#4 Model Varian INOVA (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 5.5 (±0.05)

#	Name	Isotope labeling	Concentration
1	ZK652.3	[U-99% 15N]	0.9 mM
2	ammonium acetate		10 mM
3	sodium chloride		50 mM

Sample #2

Temperature 298 (±1) K, pH 5.5 (±0.05)

#	Name	Isotope labeling	Concentration
4	ZK652.3	[U-99% 13C; U-99% 15N]	0.9 mM
5	ammonium acetate		10 mM
6	sodium chloride		50 mM

Sample #3

Temperature 298 (±1) K, pH 5.5 (±0.05)

#	Name	Isotope labeling	Concentration
7	ZK652.3	[U-10% 13C; U-99% 15N]	0.9 mM
8	ammonium acetate		10 mM
9	sodium chloride		50 mM

15 2D 1H-13C HSQC (alpha Sens. Enhanced)

Instrument

#1 Model Varian UNITY (600 MHz)

#2 Model Varian INOVA (600 MHz)

#3 Model Varian INOVA (750 MHz)

#4 Model Varian INOVA (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 5.5 (±0.05)

#	Name	Isotope labeling	Concentration
1	ZK652.3	[U-99% 15N]	0.9 mM
2	ammonium acetate		10 mM
3	sodium chloride		50 mM

Sample #2

Temperature 298 (±1) K, pH 5.5 (±0.05)

#	Name	Isotope labeling	Concentration
4	ZK652.3	[U-99% 13C; U-99% 15N]	0.9 mM
5	ammonium acetate		10 mM
6	sodium chloride		50 mM

Sample #3

Temperature 298 (±1) K, pH 5.5 (±0.05)

#	Name	Isotope labeling	Concentration
7	ZK652.3	[U-10% 13C; U-99% 15N]	0.9 mM
8	ammonium acetate		10 mM
9	sodium chloride		50 mM

16 2D 1H-13C HSQC (arom)

Instrument

#1 Model Varian UNITY (600 MHz)

#2 Model Varian INOVA (600 MHz)

#3 Model Varian INOVA (750 MHz)

#4 Model Varian INOVA (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 5.5 (±0.05)

#	Name	Isotope labeling	Concentration
1	ZK652.3	[U-99% 15N]	0.9 mM
2	ammonium acetate		10 mM
3	sodium chloride		50 mM

Sample #2

Temperature 298 (±1) K, pH 5.5 (±0.05)

#	Name	Isotope labeling	Concentration
4	ZK652.3	[U-99% 13C; U-99% 15N]	0.9 mM
5	ammonium acetate		10 mM
6	sodium chloride		50 mM

Sample #3

Temperature 298 (±1) K, pH 5.5 (±0.05)

#	Name	Isotope labeling	Concentration
7	ZK652.3	[U-10% 13C; U-99% 15N]	0.9 mM
8	ammonium acetate		10 mM
9	sodium chloride		50 mM

nullKeywords NESG, Northeast Structural Genomics, Consortium Structural Genomics

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Found 1 Document (1.877 seconds)

BMRB: 5329

Sample #1

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. C. elegans protein ZK652.3, : 1 : 2 : 1 : 59 entities Detail

Interaction partners 1. C. elegans protein ZK652.3, : 2 interactors Detail

Experiments performed 16 experiments Detail

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