BMRBj - BMREntryMaster

Found 1 Document (1.625 seconds)

BMRB: 5335

1LV3

Backbone and side chain 1H, 13C, and 15N chemical shift assignments for E.coli protein YacG

Authors

Ramelot, T.A., Kennedy, M.A.

Assembly

YacG

Entity

1. YacG (polymer, Thiol state: all other bound), 68 monomers, 7587.361 Da Detail

GSHMSETITV NCPTCGKTVV WGEISPFRPF CSKRCQLIDL GEWAAEEKRI PSSGDLSESD DWSEEPKQ

2. ZN (non-polymer), 65.409 Da

Total weight

7652.77 Da

Max. entity weight

7587.361 Da

Entity Connection

na 4 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS12:SG	2:ZN1:ZN
2	na	sing	1:CYS15:SG	2:ZN1:ZN
3	na	sing	1:CYS31:SG	2:ZN1:ZN
4	na	sing	1:CYS35:SG	2:ZN1:ZN

Source organism

Escherichia coli

Exptl. method

NMR

Refine. method

distance geometry and simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.1 %, Completeness: 86.2 %, Completeness (bb): 91.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	86.2 % (662 of 768)	85.1 % (342 of 402)	86.9 % (258 of 297)	89.9 % (62 of 69)
Backbone	91.2 % (363 of 398)	91.2 % (124 of 136)	91.0 % (181 of 199)	92.1 % (58 of 63)
Sidechain	82.9 % (359 of 433)	82.0 % (218 of 266)	85.1 % (137 of 161)	66.7 % (4 of 6)
Aromatic	78.3 % (47 of 60)	83.3 % (25 of 30)	70.4 % (19 of 27)	100.0 % (3 of 3)
Methyl	88.5 % (46 of 52)	84.6 % (22 of 26)	92.3 % (24 of 26)

1. YacG

GSHMSETITV NCPTCGKTVV WGEISPFRPF CSKRCQLIDL GEWAAEEKRI PSSGDLSESD DWSEEPKQ

Show sample condition

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	YacG	[U-13C; U-15N]	1 mM
2	phosphate buffer		25 mM
3	NaCl		450 mM

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
4	YacG	[U-15N]	1.0 ~ 3.0 mM
5	phosphate buffer		25 mM
6	NaCl		450 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.04 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.04 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.07 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.16 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1LV3, Strand ID: A Detail

Release date

2002-09-08

Citation

NMR structure of the Escherichia coli protein YacG: a novel sequence motif in the zinc-finger family of proteins
Ramelot, T.A., Cort, J.R., Yee, A.A., Semesi, A., Edwards, A.M., Arrowsmith, C.H., Kennedy, M.A.
Proteins (2002), 49, 289-293, PubMed 12211008 , DOI 10.1002/prot.10214 , Abstract

Related entities 1. YacG, : 1 : 5 : 48 entities Detail

Interaction partners 1. YacG, : 4 interactors Detail

More details for 4 interactors identified by 1 citations

Experiments performed 14 experiments Detail

1 1H-15N HSQC

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Varian Inova (750 MHz)

#3 Model Varian Inova (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	YacG	[U-13C; U-15N]	1 mM
2	phosphate buffer		25 mM
3	NaCl		450 mM

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
4	YacG	[U-15N]	1.0 ~ 3.0 mM
5	phosphate buffer		25 mM
6	NaCl		450 mM

2 1H-13C HSQC (aliph)

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Varian Inova (750 MHz)

#3 Model Varian Inova (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	YacG	[U-13C; U-15N]	1 mM
2	phosphate buffer		25 mM
3	NaCl		450 mM

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
4	YacG	[U-15N]	1.0 ~ 3.0 mM
5	phosphate buffer		25 mM
6	NaCl		450 mM

3 15N-edited NOESY-HQSC

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Varian Inova (750 MHz)

#3 Model Varian Inova (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	YacG	[U-13C; U-15N]	1 mM
2	phosphate buffer		25 mM
3	NaCl		450 mM

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
4	YacG	[U-15N]	1.0 ~ 3.0 mM
5	phosphate buffer		25 mM
6	NaCl		450 mM

4 HCC-TOCSY-NNH

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Varian Inova (750 MHz)

#3 Model Varian Inova (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	YacG	[U-13C; U-15N]	1 mM
2	phosphate buffer		25 mM
3	NaCl		450 mM

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
4	YacG	[U-15N]	1.0 ~ 3.0 mM
5	phosphate buffer		25 mM
6	NaCl		450 mM

5 HCCH-TOCSY

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Varian Inova (750 MHz)

#3 Model Varian Inova (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	YacG	[U-13C; U-15N]	1 mM
2	phosphate buffer		25 mM
3	NaCl		450 mM

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
4	YacG	[U-15N]	1.0 ~ 3.0 mM
5	phosphate buffer		25 mM
6	NaCl		450 mM

6 CN-NOESY-HSQC

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Varian Inova (750 MHz)

#3 Model Varian Inova (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	YacG	[U-13C; U-15N]	1 mM
2	phosphate buffer		25 mM
3	NaCl		450 mM

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
4	YacG	[U-15N]	1.0 ~ 3.0 mM
5	phosphate buffer		25 mM
6	NaCl		450 mM

7 HNCO

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Varian Inova (750 MHz)

#3 Model Varian Inova (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	YacG	[U-13C; U-15N]	1 mM
2	phosphate buffer		25 mM
3	NaCl		450 mM

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
4	YacG	[U-15N]	1.0 ~ 3.0 mM
5	phosphate buffer		25 mM
6	NaCl		450 mM

8 HNCACB

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Varian Inova (750 MHz)

#3 Model Varian Inova (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	YacG	[U-13C; U-15N]	1 mM
2	phosphate buffer		25 mM
3	NaCl		450 mM

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
4	YacG	[U-15N]	1.0 ~ 3.0 mM
5	phosphate buffer		25 mM
6	NaCl		450 mM

9 CBCA(CO)NH

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Varian Inova (750 MHz)

#3 Model Varian Inova (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	YacG	[U-13C; U-15N]	1 mM
2	phosphate buffer		25 mM
3	NaCl		450 mM

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
4	YacG	[U-15N]	1.0 ~ 3.0 mM
5	phosphate buffer		25 mM
6	NaCl		450 mM

10 CCC-TOCSY-NNH

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Varian Inova (750 MHz)

#3 Model Varian Inova (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	YacG	[U-13C; U-15N]	1 mM
2	phosphate buffer		25 mM
3	NaCl		450 mM

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
4	YacG	[U-15N]	1.0 ~ 3.0 mM
5	phosphate buffer		25 mM
6	NaCl		450 mM

11 HNHA

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Varian Inova (750 MHz)

#3 Model Varian Inova (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	YacG	[U-13C; U-15N]	1 mM
2	phosphate buffer		25 mM
3	NaCl		450 mM

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
4	YacG	[U-15N]	1.0 ~ 3.0 mM
5	phosphate buffer		25 mM
6	NaCl		450 mM

12 4D CC-NOESY

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Varian Inova (750 MHz)

#3 Model Varian Inova (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	YacG	[U-13C; U-15N]	1 mM
2	phosphate buffer		25 mM
3	NaCl		450 mM

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
4	YacG	[U-15N]	1.0 ~ 3.0 mM
5	phosphate buffer		25 mM
6	NaCl		450 mM

13 1H-13C HSQC (alpha)

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Varian Inova (750 MHz)

#3 Model Varian Inova (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	YacG	[U-13C; U-15N]	1 mM
2	phosphate buffer		25 mM
3	NaCl		450 mM

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
4	YacG	[U-15N]	1.0 ~ 3.0 mM
5	phosphate buffer		25 mM
6	NaCl		450 mM

14 1H-13C HSQC (arom)

Instrument

#1 Model Varian Inova (600 MHz)

#2 Model Varian Inova (750 MHz)

#3 Model Varian Inova (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	YacG	[U-13C; U-15N]	1 mM
2	phosphate buffer		25 mM
3	NaCl		450 mM

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
4	YacG	[U-15N]	1.0 ~ 3.0 mM
5	phosphate buffer		25 mM
6	NaCl		450 mM

null

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (1.625 seconds)

BMRB: 5335

Sample #1

Sample #2

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. YacG, : 1 : 5 : 48 entities Detail

Interaction partners 1. YacG, : 4 interactors Detail

Experiments performed 14 experiments Detail

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2