BMRBj - BMREntryMaster

Found 1 Document (1.854 seconds)

BMRB: 5338

1L3Y

Integrin EGF-like module 3 from the beta-2 subunit

Authors

Beglova, N., Blacklow, S.C., Takagi, J., Springer, T.A.

Assembly

IEGF-3

Entity

1. IEGF-3 (polymer, Thiol state: all disulfide bound), 41 monomers, 4327.864 Da Detail

ECDTINCERY NGQVCGGPGR GLCFCGKCRC HPGFEGSACQ A

Formula weight

4327.864 Da

Entity Connection

disulfide 4 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS2:SG	1:CYS25:SG
2	disulfide	sing	1:CYS7:SG	1:CYS23:SG
3	disulfide	sing	1:CYS15:SG	1:CYS28:SG
4	disulfide	sing	1:CYS30:SG	1:CYS39:SG

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.6 %, Completeness: 96.2 %, Completeness (bb): 97.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹⁵N
All	96.2 % (255 of 265)	95.9 % (213 of 222)	97.7 % (42 of 43)
Backbone	97.6 % (124 of 127)	97.7 % (86 of 88)	97.4 % (38 of 39)
Sidechain	94.9 % (131 of 138)	94.8 % (127 of 134)	100.0 % (4 of 4)
Aromatic	100.0 % (16 of 16)	100.0 % (16 of 16)
Methyl	100.0 % (9 of 9)	100.0 % (9 of 9)

1. integrin

ECDTINCERY NGQVCGGPGR GLCFCGKCRC HPGFEGSACQ A

Show sample condition

Sample

Pressure 1 atm, Temperature 298 (±1) K, pH 6.2 (±0.05)

#	Name	Isotope labeling	Concentration
1	integrin	[U-15N]	1 mM
2	NaPO4		5 mM
3	H2O		90 %
4	D2O		10 %

Protein Blocks Logo

Calculated from 15 models in PDB: 1L3Y, Strand ID: A Detail

Release date

2002-04-02

Citation

Cysteine-rich module structure reveals a fulcrum for integrin rearrangement upon activation
Beglova, N., Blacklow, S.C., Takagi, J., Springer, T.A.
Nat. Struct. Biol. (2002), 9, 282-287, PubMed 11896403 , DOI 10.1038/nsb779 , Abstract

Related entities 1. IEGF-3, : 1 : 10 : 225 entities Detail

Interaction partners 1. IEGF-3, : 22 interactors Detail

More details for 22 interactors identified by 13 citations

Experiments performed 6 experiments Detail

1 3D 15N-separated NOESY

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 (±1) K, pH 6.2 (±0.05)

#	Name	Isotope labeling	Concentration
1	integrin	[U-15N]	1 mM
2	NaPO4		5 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 (±1) K, pH 6.2 (±0.05)

#	Name	Concentration
5	integrin	1 mM
6	NaPO4	5 mM
7	D2O	100 %

2 3D 15N-separated TOCSY

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 (±1) K, pH 6.2 (±0.05)

#	Name	Isotope labeling	Concentration
1	integrin	[U-15N]	1 mM
2	NaPO4		5 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 (±1) K, pH 6.2 (±0.05)

#	Name	Concentration
5	integrin	1 mM
6	NaPO4	5 mM
7	D2O	100 %

3 HMQC-J

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 (±1) K, pH 6.2 (±0.05)

#	Name	Isotope labeling	Concentration
1	integrin	[U-15N]	1 mM
2	NaPO4		5 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 (±1) K, pH 6.2 (±0.05)

#	Name	Concentration
5	integrin	1 mM
6	NaPO4	5 mM
7	D2O	100 %

4 1H-NOESY

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 (±1) K, pH 6.2 (±0.05)

#	Name	Isotope labeling	Concentration
1	integrin	[U-15N]	1 mM
2	NaPO4		5 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 (±1) K, pH 6.2 (±0.05)

#	Name	Concentration
5	integrin	1 mM
6	NaPO4	5 mM
7	D2O	100 %

5 1H-TOCSY

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 (±1) K, pH 6.2 (±0.05)

#	Name	Isotope labeling	Concentration
1	integrin	[U-15N]	1 mM
2	NaPO4		5 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 (±1) K, pH 6.2 (±0.05)

#	Name	Concentration
5	integrin	1 mM
6	NaPO4	5 mM
7	D2O	100 %

6 DQF-COSY

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 298 (±1) K, pH 6.2 (±0.05)

#	Name	Isotope labeling	Concentration
1	integrin	[U-15N]	1 mM
2	NaPO4		5 mM
3	H2O		90 %
4	D2O		10 %

Sample #2

Pressure 1 atm, Temperature 298 (±1) K, pH 6.2 (±0.05)

#	Name	Concentration
5	integrin	1 mM
6	NaPO4	5 mM
7	D2O	100 %

nullKeywords EGF-like module, beta-2 subunit, cell adhesion, cysteine-rich module, integrin

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Found 1 Document (1.854 seconds)

BMRB: 5338

Sample

Related entities 1. IEGF-3, : 1 : 10 : 225 entities Detail

Interaction partners 1. IEGF-3, : 22 interactors Detail

Experiments performed 6 experiments Detail

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