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BMRB: 5340

2BID

Solution Structure for BID, and Intracellular Amplifier of Apoptotic Signaling

Authors

Chou, J.J., Li, H., Salvesen, G.S., Yuan, J., Wagner, G.

Assembly

proapoptotic bid

Entity

1. proapoptotic bid (polymer, Thiol state: all free), 197 monomers, 22138.58 Da Detail

GSMDCEVNNG SSLRDECITN LLVFGFLQSC SDNSFRRELD ALGHELPVLA PQWEGYDELQ TDGNRSSHSR LGRIEADSES QEDIIRNIAR HLAQVGDSMD RSIPPGLVNG LALQLRNTSR SEEDRNRDLA TALEQLLQAY PRDMEKEKTM LVLALLLAKK VASHTPSLLR DVFHTTVNFI NQNLRTYVRS LARNGMD

Formula weight

22138.58 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

DISTANCE GEOMETRY

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 91.9 %, Completeness: 47.3 %, Completeness (bb): 78.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	47.3 % (1061 of 2242)	26.8 % (313 of 1169)	66.2 % (569 of 859)	83.6 % (179 of 214)
Backbone	78.4 % (917 of 1170)	52.6 % (210 of 399)	91.2 % (529 of 580)	93.2 % (178 of 191)
Sidechain	25.0 % (315 of 1258)	13.4 % (103 of 770)	45.4 % (211 of 465)	4.3 % (1 of 23)
Aromatic	7.5 % (8 of 106)	7.5 % (4 of 53)	7.7 % (4 of 52)	0.0 % (0 of 1)
Methyl	21.4 % (48 of 224)	16.1 % (18 of 112)	26.8 % (30 of 112)

1. bid

Show sample condition

Sample

Temperature 298 (±2) K, pH 6.5 (±0.3)

#	Name	Isotope labeling	Type	Concentration
1	bid	[U-95% 13C; U-95% 15N; U-85% 2H]		1.0 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.8 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.8 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.4 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 15 models in PDB: 2BID, Strand ID: A Detail

Release date

2002-06-06

Citation 1

Solution structure of BID, an intracellular amplifier of apoptotic signaling
Chou, J.J., Li, H., Salvesen, G.S., Yuan, J., Wagner, G.
Cell (1999), 96, 615-624, PubMed 10089877 , DOI: , Abstract

Show more 1 citaion

Citation 2

Cleavage of BID by caspase 8 mediates the mitochondrial damage in the Fas pathway of apoptosis
Li, H., Zhu, H., Xu, C.J., Yuan, J.
Cell (1998), 94, 491-501, PubMed 9727492 , DOI: , Abstract

Hide non-primary 1 citaion

Related entities 1. proapoptotic bid, : 1 : 2 : 7 entities Detail

Interaction partners 1. proapoptotic bid, : 38 interactors Detail

More details for 38 interactors identified by 15 citations

Experiments performed 11 experiments Detail

1 HNCA