BMRB: 5346

Solution Structure of a Novel Disintegrin, Salmosin from Agkistrondon halys Venom

Authors

Shin, J., Hong, S., Chung, K., Kang, I., Jang, Y., Kim, D., Lee, W.

Assembly

platelet aggregation inhibitor disintegrin

Entity

1. platelet aggregation inhibitor disintegrin (polymer, Thiol state: all disulfide bound), 73 monomers, 7765.616 Da Detail

EAGEECDCGS PGNPCCDAAT CKLRQGAQCA EGLCCDQCRF MKEGTICRRA RGDDLDDYCN GISAGCPRNP FHA

Formula weight

7765.616 Da

Entity Connection

disulfide 6 Detail

---

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS6:SG	1:CYS15:SG
2	disulfide	sing	1:CYS8:SG	1:CYS16:SG
3	disulfide	sing	1:CYS21:SG	1:CYS35:SG
4	disulfide	sing	1:CYS29:SG	1:CYS59:SG
5	disulfide	sing	1:CYS34:SG	1:CYS38:SG
6	disulfide	sing	1:CYS47:SG	1:CYS66:SG

Source organism

Gloydius halys

Exptl. method

NMR

Refine. method

distance geometry, simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.6 %, Completeness: 88.0 %, Completeness (bb): 96.0 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H
All	88.0 % (344 of 391)	88.0 % (344 of 391)
Backbone	96.0 % (145 of 151)	96.0 % (145 of 151)
Sidechain	82.9 % (199 of 240)	82.9 % (199 of 240)
Aromatic	75.0 % (12 of 16)	75.0 % (12 of 16)
Methyl	100.0 % (20 of 20)	100.0 % (20 of 20)

1. salmosin

EAGEECDCGS PGNPCCDAAT CKLRQGAQCA EGLCCDQCRF MKEGTICRRA RGDDLDDYCN GISAGCPRNP FHA

Show sample condition

Sample

Pressure 1 atm, Temperature 298 (±0.1) K, pH 7 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	salmosin			1.0 mM
2	H2O			90 %
3	D2O			10 %

Hide sample codition

Protein Blocks Logo

Calculated from 20 models in PDB: 1L3X, Strand ID: A Detail

---

Release date

2002-04-18

Citation

Solution structure of a novel disintegrin, salmosin, from Agkistrondon halys venom
Shin, J., Hong, S., Chung, K., Kang, I., Jang, Y., Kim, D., Lee, W.
Biochemistry (2003), 42, 14408-14415, PubMed 14661951 , DOI 10.1021/bi0300276 , Abstract

Related entities 1. platelet aggregation inhibitor disintegrin, : 1 : 1 : 1 : 244 entities Detail

Experiments performed 3 experiments Detail