BMRBj - BMREntryMaster

Found 1 Document (2.695 seconds)

BMRB: 5353

1LKJ

1H, 15N and 13C resonance assignments of yeast Saccharomyces cerevisiae calmodulin in the Ca2+-free state

Authors

Ishida, H.

Assembly

yeast Saccharomyces cerevisiae calmodulin

Entity

1. yeast Saccharomyces cerevisiae calmodulin (polymer, Thiol state: not present), 146 monomers, 16003.48 Da Detail

SSNLTEEQIA EFKEAFALFD KDNNGSISSS ELATVMRSLG LSPSEAEVND LMNEIDVDGN HQIEFSEFLA LMSRQLKSND SEQELLEAFK VFDKNGDGLI SAAELKHVLT SIGEKLTDAE VDDMLREVSD GSGEINIQQF AALLSK

Formula weight

16003.48 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.6 %, Completeness: 31.2 %, Completeness (bb): 66.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹⁵N
All	31.2 % (315 of 1009)	20.6 % (175 of 849)	87.5 % (140 of 160)
Backbone	66.0 % (293 of 444)	51.2 % (153 of 299)	96.6 % (140 of 145)
Sidechain	3.9 % (22 of 565)	4.0 % (22 of 550)	0.0 % (0 of 15)
Aromatic	11.4 % (5 of 44)	11.4 % (5 of 44)
Methyl	2.4 % (2 of 82)	2.4 % (2 of 82)

1. yeast calmodulin

SSNLTEEQIA EFKEAFALFD KDNNGSISSS ELATVMRSLG LSPSEAEVND LMNEIDVDGN HQIEFSEFLA LMSRQLKSND SEQELLEAFK VFDKNGDGLI SAAELKHVLT SIGEKLTDAE VDDMLREVSD GSGEINIQQF AALLSK

Show sample condition

Sample

Pressure 1 atm, Temperature 303 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	yeast calmodulin	[U-13C; U-15N]		1.0 mM
2	yeast calmodulin	[U-13C; U-15N]		1.4 mM

Protein Blocks Logo

Calculated from 31 models in PDB: 1LKJ, Strand ID: A Detail

Release date

2002-09-24

Citation

Letter to the Editor: 1H, 15N and 13C resonance assignments of yeast Saccharomyces cerevisiae calmodulin in the Ca2+-free state
Ishida, H., Nakashima, K., Kumaki, Y., Nakata, M., Hikichi, K., Yazawa, M.
J. Biomol. NMR (2002), 23, 323-324

Related entities 1. yeast Saccharomyces cerevisiae calmodulin, : 1 : 4 : 293 entities Detail

Interaction partners 1. yeast Saccharomyces cerevisiae calmodulin, : 75 interactors Detail

More details for 75 interactors identified by 10 citations

Experiments performed 11 experiments Detail

1 HSQC

Instrument

#1 Model Varian UNITY (500 MHz)

#2 Model JEOL JNM (600 MHz)

Sample #1

Pressure 1 atm, Temperature 303 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	yeast calmodulin	[U-13C; U-15N]		1.0 mM
2	yeast calmodulin	[U-13C; U-15N]		1.4 mM

Sample #2

Pressure 1 atm, Temperature 303 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	yeast calmodulin	[U-15N]		1.0 mM
4	yeast calmodulin	[U-15N]		1.4 mM

2 HNCA

Instrument

#1 Model Varian UNITY (500 MHz)

#2 Model JEOL JNM (600 MHz)

Sample #1

Pressure 1 atm, Temperature 303 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	yeast calmodulin	[U-13C; U-15N]		1.0 mM
2	yeast calmodulin	[U-13C; U-15N]		1.4 mM

Sample #2

Pressure 1 atm, Temperature 303 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	yeast calmodulin	[U-15N]		1.0 mM
4	yeast calmodulin	[U-15N]		1.4 mM

3 HN(CO)CA

Instrument

#1 Model Varian UNITY (500 MHz)

#2 Model JEOL JNM (600 MHz)

Sample #1

Pressure 1 atm, Temperature 303 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	yeast calmodulin	[U-13C; U-15N]		1.0 mM
2	yeast calmodulin	[U-13C; U-15N]		1.4 mM

Sample #2

Pressure 1 atm, Temperature 303 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	yeast calmodulin	[U-15N]		1.0 mM
4	yeast calmodulin	[U-15N]		1.4 mM

4 HNCACB

Instrument

#1 Model Varian UNITY (500 MHz)

#2 Model JEOL JNM (600 MHz)

Sample #1

Pressure 1 atm, Temperature 303 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	yeast calmodulin	[U-13C; U-15N]		1.0 mM
2	yeast calmodulin	[U-13C; U-15N]		1.4 mM

Sample #2

Pressure 1 atm, Temperature 303 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	yeast calmodulin	[U-15N]		1.0 mM
4	yeast calmodulin	[U-15N]		1.4 mM

5 CBCA(CO)NH

Instrument

#1 Model Varian UNITY (500 MHz)

#2 Model JEOL JNM (600 MHz)

Sample #1

Pressure 1 atm, Temperature 303 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	yeast calmodulin	[U-13C; U-15N]		1.0 mM
2	yeast calmodulin	[U-13C; U-15N]		1.4 mM

Sample #2

Pressure 1 atm, Temperature 303 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	yeast calmodulin	[U-15N]		1.0 mM
4	yeast calmodulin	[U-15N]		1.4 mM

6 HNHA

Instrument

#1 Model Varian UNITY (500 MHz)

#2 Model JEOL JNM (600 MHz)

Sample #1

Pressure 1 atm, Temperature 303 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	yeast calmodulin	[U-13C; U-15N]		1.0 mM
2	yeast calmodulin	[U-13C; U-15N]		1.4 mM

Sample #2

Pressure 1 atm, Temperature 303 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	yeast calmodulin	[U-15N]		1.0 mM
4	yeast calmodulin	[U-15N]		1.4 mM

7 HNCO

Instrument

#1 Model Varian UNITY (500 MHz)

#2 Model JEOL JNM (600 MHz)

Sample #1

Pressure 1 atm, Temperature 303 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	yeast calmodulin	[U-13C; U-15N]		1.0 mM
2	yeast calmodulin	[U-13C; U-15N]		1.4 mM

Sample #2

Pressure 1 atm, Temperature 303 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	yeast calmodulin	[U-15N]		1.0 mM
4	yeast calmodulin	[U-15N]		1.4 mM

8 HBHA(CBCACO)NH

Instrument

#1 Model Varian UNITY (500 MHz)

#2 Model JEOL JNM (600 MHz)

Sample #1

Pressure 1 atm, Temperature 303 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	yeast calmodulin	[U-13C; U-15N]		1.0 mM
2	yeast calmodulin	[U-13C; U-15N]		1.4 mM

Sample #2

Pressure 1 atm, Temperature 303 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	yeast calmodulin	[U-15N]		1.0 mM
4	yeast calmodulin	[U-15N]		1.4 mM

9 H(CCO)NH

Instrument

#1 Model Varian UNITY (500 MHz)

#2 Model JEOL JNM (600 MHz)

Sample #1

Pressure 1 atm, Temperature 303 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	yeast calmodulin	[U-13C; U-15N]		1.0 mM
2	yeast calmodulin	[U-13C; U-15N]		1.4 mM

Sample #2

Pressure 1 atm, Temperature 303 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	yeast calmodulin	[U-15N]		1.0 mM
4	yeast calmodulin	[U-15N]		1.4 mM

10 C(CO)NH

Instrument

#1 Model Varian UNITY (500 MHz)

#2 Model JEOL JNM (600 MHz)

Sample #1

Pressure 1 atm, Temperature 303 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	yeast calmodulin	[U-13C; U-15N]		1.0 mM
2	yeast calmodulin	[U-13C; U-15N]		1.4 mM

Sample #2

Pressure 1 atm, Temperature 303 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	yeast calmodulin	[U-15N]		1.0 mM
4	yeast calmodulin	[U-15N]		1.4 mM

11 HCCH-TOCSY

Instrument

#1 Model Varian UNITY (500 MHz)

#2 Model JEOL JNM (600 MHz)

Sample #1

Pressure 1 atm, Temperature 303 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	yeast calmodulin	[U-13C; U-15N]		1.0 mM
2	yeast calmodulin	[U-13C; U-15N]		1.4 mM

Sample #2

Pressure 1 atm, Temperature 303 (±0.1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	yeast calmodulin	[U-15N]		1.0 mM
4	yeast calmodulin	[U-15N]		1.4 mM

nullKeywords Yeast Saccharomyces cerevisiae Calmodulin, EF-hand

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (2.695 seconds)

BMRB: 5353

Sample

Related entities 1. yeast Saccharomyces cerevisiae calmodulin, : 1 : 4 : 293 entities Detail

Interaction partners 1. yeast Saccharomyces cerevisiae calmodulin, : 75 interactors Detail

Experiments performed 11 experiments Detail

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2