BMRB: 5356

Backbone and sidechain chemical shift assignments for GS-a3W protein

Authors

Dai, Q., Tommos, C., Fuentes, E.J., Dutton, P.LESLIE., Wand, A.JOSHUA.

Assembly

GS-a3W protein

Entity

1. GS-a3W protein (polymer, Thiol state: not present), 67 monomers, 7543.746 Da Detail

GSRVKALEEK VKALEEKVKA LGGGGRIEEL KKKWEELKKK IEELGGGGEV KKVEEEVKKL EEEIKKL

Formula weight

7543.746 Da

Exptl. method

NMR

Refine. method

Torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.5 %, Completeness: 98.8 %, Completeness (bb): 98.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	98.8 % (819 of 829)	98.7 % (441 of 447)	99.4 % (312 of 314)	97.1 % (66 of 68)
Backbone	98.0 % (394 of 402)	97.2 % (139 of 143)	99.0 % (190 of 192)	97.0 % (65 of 67)
Sidechain	99.6 % (483 of 485)	99.3 % (302 of 304)	100.0 % (180 of 180)	100.0 % (1 of 1)
Aromatic	100.0 % (12 of 12)	100.0 % (6 of 6)	100.0 % (5 of 5)	100.0 % (1 of 1)
Methyl	100.0 % (74 of 74)	100.0 % (37 of 37)	100.0 % (37 of 37)

1. GS-a3W

GSRVKALEEK VKALEEKVKA LGGGGRIEEL KKKWEELKKK IEELGGGGEV KKVEEEVKKL EEEIKKL

Show sample condition

Sample

Temperature 301.0 (±0.1) K

#	Name	Isotope labeling	Type	Concentration
1	GS-a3W	[U-13C; U-15N]		0.83 mM

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Protein Blocks Logo

Calculated from 16 models in PDB: 1LQ7, Strand ID: A Detail

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Release date

2003-01-05

Citation

Structure of a de novo designed protein model of radical enzymes
Dai, Q., Tommos, C., Fuentes, E.J., Dutton, P.LESLIE., Wand, A.JOSHUA.
J. Am. Chem. Soc. (2002), 124, 10952-10953, PubMed 12224922 , Abstract

Related entities 1. GS-a3W protein, : 1 : 1 : 2 : 35 entities Detail