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BMRB: 5361

1IV6

Solution Structure of the DNA Complex of Human TRF1

Authors

Nishikawa, T., Okamura, H., Nagadoi, A., Konig, P., Rhodes, D., Nishimura, Y.

Assembly

TELOMERE REPEAT BINDING FACTOR 1/DNA Complex

Entity

1. Telomeric repeat binding factor 1 (polymer, Thiol state: not present), 70 monomers, 8578.787 Da Detail

MTPEKHRARK RQAWLWEEDK NLRSGVRKYG EGNWSKILLH YKFNNRTSVM LKDRWRTMKK LKLISSDSED

2. none (polymer), 13 monomers, 4165.640 Da Detail

GTTAGGGTTA GGG

3. none (polymer), 13 monomers, 3903.500 Da Detail

CCCTAACCCT AAC

Total weight

16647.928 Da

Max. entity weight

8578.787 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

distance geometry/simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 69.8 %, Completeness: 56.3 %, Completeness (bb): 63.8 % Detail

Polymer type: polypeptide(L) polydeoxyribonucleotide

	Total	¹H	¹³C	¹⁵N
All	56.3 % (647 of 1149)	53.9 % (395 of 733)	55.0 % (186 of 338)	84.6 % (66 of 78)
Backbone	63.8 % (383 of 600)	41.4 % (134 of 324)	88.4 % (183 of 207)	95.7 % (66 of 69)
Sidechain	51.5 % (317 of 616)	63.8 % (261 of 409)	28.3 % (56 of 198)	0.0 % (0 of 9)
Aromatic	29.1 % (39 of 134)	41.9 % (39 of 93)	0.0 % (0 of 37)	0.0 % (0 of 4)
Methyl	46.7 % (28 of 60)	78.8 % (26 of 33)	7.4 % (2 of 27)

1. Telomeric repeat binding factor 1

MTPEKHRARK RQAWLWEEDK NLRSGVRKYG EGNWSKILLH YKFNNRTSVM LKDRWRTMKK LKLISSDSED

2. none

GTTAGGGTTA GGG

3. none

CCCTAACCCT AAC

Show sample condition

Sample

Pressure 1 atm, Temperature 305 K, pH 6.8 (±0.05)

#	Name	Isotope labeling	Concentration
1	Telomeric repeat binding factor 1	[U-15N; U-13C]	1.5 ~ 2.5 mM
2	none		1.5 ~ 2.5 mM
3	none		1.5 ~ 2.5 mM
4	Phosphate buffer		5 mM
5	NaCl		10 mM

Chem. Shift Complete2

Sequence coverage: 22.9 %, Completeness: 35.1 %, Completeness (bb): 40.4 % Detail

Polymer type: polypeptide(L) polydeoxyribonucleotide

	Total	¹H	¹³C	¹⁵N
All	35.1 % (807 of 2298)	36.6 % (537 of 1466)	29.6 % (200 of 676)	44.9 % (70 of 156)
Backbone	40.4 % (485 of 1200)	34.3 % (222 of 648)	46.6 % (193 of 414)	50.7 % (70 of 138)
Sidechain	30.2 % (372 of 1232)	37.9 % (310 of 818)	15.7 % (62 of 396)	0.0 % (0 of 18)
Aromatic	23.1 % (62 of 268)	33.3 % (62 of 186)	0.0 % (0 of 74)	0.0 % (0 of 8)
Methyl	26.7 % (32 of 120)	45.5 % (30 of 66)	3.7 % (2 of 54)

1. Telomeric repeat binding factor 1

MTPEKHRARK RQAWLWEEDK NLRSGVRKYG EGNWSKILLH YKFNNRTSVM LKDRWRTMKK LKLISSDSED

2. none

GTTAGGGTTA GGG

3. none

CCCTAACCCT AAC

Show sample condition

Sample

Pressure 1 atm, Temperature 305 K, pH 6.8 (±0.05)

#	Name	Isotope labeling	Concentration
1	Telomeric repeat binding factor 1	[U-15N; U-13C]	1.5 ~ 2.5 mM
2	none		1.5 ~ 2.5 mM
3	none		1.5 ~ 2.5 mM
4	Phosphate buffer		5 mM
5	NaCl		10 mM

Chem. Shift Complete3

Sequence coverage: 26.0 %, Completeness: 28.4 %, Completeness (bb): 33.2 % Detail

Polymer type: polypeptide(L) polydeoxyribonucleotide

	Total	¹H	¹³C	¹⁵N
All	28.4 % (980 of 3447)	30.4 % (669 of 2199)	22.6 % (229 of 1014)	35.0 % (82 of 234)
Backbone	33.2 % (598 of 1800)	31.5 % (306 of 972)	34.0 % (211 of 621)	39.1 % (81 of 207)
Sidechain	23.7 % (438 of 1848)	29.2 % (358 of 1227)	13.3 % (79 of 594)	3.7 % (1 of 27)
Aromatic	24.9 % (100 of 402)	33.7 % (94 of 279)	4.5 % (5 of 111)	8.3 % (1 of 12)
Methyl	20.0 % (36 of 180)	33.3 % (33 of 99)	3.7 % (3 of 81)

1. Telomeric repeat binding factor 1

MTPEKHRARK RQAWLWEEDK NLRSGVRKYG EGNWSKILLH YKFNNRTSVM LKDRWRTMKK LKLISSDSED

2. none

GTTAGGGTTA GGG

3. none

CCCTAACCCT AAC

Show sample condition

Sample

Pressure 1 atm, Temperature 305 K, pH 6.8 (±0.05)

#	Name	Isotope labeling	Concentration
1	Telomeric repeat binding factor 1	[U-15N; U-13C]	1.5 ~ 2.5 mM
2	none		1.5 ~ 2.5 mM
3	none		1.5 ~ 2.5 mM
4	Phosphate buffer		5 mM
5	NaCl		10 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	1.0 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

Referencing offset: 2.29 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	1.0 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

Referencing offset: 2.29 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	1.0 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

Referencing offset: -0.01 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	1.0 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

Referencing offset: 2.35 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1IV6, Strand ID: A Detail

Release date

2002-06-25

Citation

Solution structure of a telomeric DNA complex of human TRF1
Nishikawa, T., Okamura, H., Nagadoi, A., Konig, P., Rhodes, D., Nishimura, Y.
Structure (2001), 9, 1237-1251, PubMed 11738049 , DOI: , Abstract

Entries sharing articles BMRB: 1, Swiss-Prot: 1 entries Detail

  BMRB: 5317 released on 2002-04-07
    Title Solution structure of the DNA binding domain of human TRF1
  Swiss-Prot: P54274 released on 1996-10-01
    Title TERF1_HUMAN Entity Telomeric repeat-binding factor 1

Related entities 1. Telomeric repeat binding factor 1, : 1 : 2 : 1 : 49 entities Detail

Related entities 2. none, : 2 entities Detail

Related entities 3. none, : 2 entities Detail

Interaction partners 1. Telomeric repeat binding factor 1, : 211 interactors Detail

More details for 211 interactors identified by 23 citations

Experiments performed 13 experiments Detail

1 3D HNCA