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BMRB: 5363

1LO1

1H, 13C, and 15N Chemical shifts for hERR2 Protein, 1H chemical shifts for DNA

Authors

Gearhart, M.D., Holmbeck, S.M.A., Evans, R.M., Dyson, H.JANE., Wright, P.E.

Assembly

hERR2-DNA complex

Entity

1. Estrogen Related Receptor 2 (polymer, Thiol state: not reported), 98 monomers, 11124.92 Da Detail

AIPKRLCLVC GDIASGYHYG VASCEACKAF FKRTIQGNIE YSCPATNECE ITKRRRKSCQ ACRFMKALKV GMLKEGVRLD RVRGGRQKYK RRLDSENS

2. DNA Strand1 (polymer, Thiol state: not present), 13 monomers, 4039.571 Da Detail

GCTCAAGGTC ACG

3. DNA Strand2 (polymer, Thiol state: not present), 13 monomers, 4030.557 Da Detail

CGTGACCTTG AGC

4. ZN (non-polymer), 65.409 Da

Total weight

19260.457 Da

Max. entity weight

11124.92 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

distance geometry

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 78.2 %, Completeness: 71.5 %, Completeness (bb): 62.9 % Detail

Polymer type: polypeptide(L) polydeoxyribonucleotide

	Total	¹H	¹³C	¹⁵N
All	71.5 % (1002 of 1402)	66.3 % (573 of 864)	76.4 % (333 of 436)	94.1 % (96 of 102)
Backbone	62.9 % (482 of 766)	50.8 % (195 of 384)	68.9 % (197 of 286)	93.8 % (90 of 96)
Sidechain	83.5 % (606 of 726)	78.8 % (378 of 480)	92.5 % (222 of 240)	100.0 % (6 of 6)
Aromatic	50.0 % (59 of 118)	36.5 % (31 of 85)	84.8 % (28 of 33)
Methyl	92.3 % (84 of 91)	87.5 % (42 of 48)	97.7 % (42 of 43)

1. Estrogen Related Receptor 2

AIPKRLCLVC GDIASGYHYG VASCEACKAF FKRTIQGNIE YSCPATNECE ITKRRRKSCQ ACRFMKALKV GMLKEGVRLD RVRGGRQKYK RRLDSENS

2. DNA Strand1

GCTCAAGGTC ACG

3. DNA Strand2

CGTGACCTTG AGC

Show sample condition

Sample #1

Temperature 310 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
1	Estrogen Related Receptor 2	[U-13C; U-15N]	0.5 mM
2	DNA Strand1		0.5 mM
3	DNA Strand2		0.5 mM
4	ZINC (II) ION		0.5 mM
5	K2SO4		20 mM
6	ZnSO4		50 mM
7	dithiothreitol (DTT)		2 mM
8	NaN3		0.001 %

Sample #2

Temperature 310 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
9	Estrogen Related Receptor 2	[U-15N]	0.5 mM
10	DNA Strand1		0.5 mM
11	DNA Strand2		0.5 mM
12	ZINC (II) ION		0.5 mM
13	K2SO4		20 mM
14	ZnSO4		50 mM
15	dithiothreitol (DTT)		2 mM
16	NaN3		0.001 %

Chem. Shift Complete2

Sequence coverage: 36.3 %, Completeness: 47.9 %, Completeness (bb): 45.4 % Detail

Polymer type: polypeptide(L) polydeoxyribonucleotide

	Total	¹H	¹³C	¹⁵N
All	47.9 % (1343 of 2804)	45.7 % (790 of 1728)	49.3 % (430 of 872)	60.3 % (123 of 204)
Backbone	45.4 % (695 of 1532)	40.0 % (307 of 768)	47.4 % (271 of 572)	60.9 % (117 of 192)
Sidechain	52.2 % (758 of 1452)	50.3 % (483 of 960)	56.0 % (269 of 480)	50.0 % (6 of 12)
Aromatic	42.8 % (101 of 236)	37.6 % (64 of 170)	56.1 % (37 of 66)
Methyl	53.3 % (97 of 182)	51.0 % (49 of 96)	55.8 % (48 of 86)

1. Estrogen Related Receptor 2

AIPKRLCLVC GDIASGYHYG VASCEACKAF FKRTIQGNIE YSCPATNECE ITKRRRKSCQ ACRFMKALKV GMLKEGVRLD RVRGGRQKYK RRLDSENS

2. DNA Strand1

GCTCAAGGTC ACG

3. DNA Strand2

CGTGACCTTG AGC

Show sample condition

Sample #1

Temperature 310 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
1	Estrogen Related Receptor 2	[U-13C; U-15N]	0.5 mM
2	DNA Strand1		0.5 mM
3	DNA Strand2		0.5 mM
4	ZINC (II) ION		0.5 mM
5	K2SO4		20 mM
6	ZnSO4		50 mM
7	dithiothreitol (DTT)		2 mM
8	NaN3		0.001 %

Sample #2

Temperature 310 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
9	Estrogen Related Receptor 2	[U-15N]	0.5 mM
10	DNA Strand1		0.5 mM
11	DNA Strand2		0.5 mM
12	ZINC (II) ION		0.5 mM
13	K2SO4		20 mM
14	ZnSO4		50 mM
15	dithiothreitol (DTT)		2 mM
16	NaN3		0.001 %

Chem. Shift Complete3

Sequence coverage: 25.0 %, Completeness: 37.1 %, Completeness (bb): 36.2 % Detail

Polymer type: polypeptide(L) polydeoxyribonucleotide

	Total	¹H	¹³C	¹⁵N
All	37.1 % (1562 of 4206)	36.5 % (945 of 2592)	36.5 % (478 of 1308)	45.4 % (139 of 306)
Backbone	36.2 % (833 of 2298)	34.2 % (394 of 1152)	35.8 % (307 of 858)	45.8 % (132 of 288)
Sidechain	39.1 % (852 of 2178)	38.3 % (551 of 1440)	40.8 % (294 of 720)	38.9 % (7 of 18)
Aromatic	35.6 % (126 of 354)	34.9 % (89 of 255)	37.4 % (37 of 99)
Methyl	43.2 % (118 of 273)	43.1 % (62 of 144)	43.4 % (56 of 129)

1. Estrogen Related Receptor 2

AIPKRLCLVC GDIASGYHYG VASCEACKAF FKRTIQGNIE YSCPATNECE ITKRRRKSCQ ACRFMKALKV GMLKEGVRLD RVRGGRQKYK RRLDSENS

2. DNA Strand1

GCTCAAGGTC ACG

3. DNA Strand2

CGTGACCTTG AGC

Show sample condition

Sample #1

Temperature 310 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
1	Estrogen Related Receptor 2	[U-13C; U-15N]	0.5 mM
2	DNA Strand1		0.5 mM
3	DNA Strand2		0.5 mM
4	ZINC (II) ION		0.5 mM
5	K2SO4		20 mM
6	ZnSO4		50 mM
7	dithiothreitol (DTT)		2 mM
8	NaN3		0.001 %

Sample #2

Temperature 310 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
9	Estrogen Related Receptor 2	[U-15N]	0.5 mM
10	DNA Strand1		0.5 mM
11	DNA Strand2		0.5 mM
12	ZINC (II) ION		0.5 mM
13	K2SO4		20 mM
14	ZnSO4		50 mM
15	dithiothreitol (DTT)		2 mM
16	NaN3		0.001 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 2.04 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 2.04 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.03 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1LO1, Strand ID: A Detail

Release date

2003-04-09

Citation

Monomeric complex of human orphan estrogen related receptor-2 with DNA: a pseudo-dimer interface mediates extended half-site recognition
Gearhart, M.D., Holmbeck, S.M.A., Evans, R.M., Dyson, H.JANE., Wright, P.E.
J. Mol. Biol. (2003), 327, 819-832, PubMed 12654265 , DOI 10.1016/s0022-2836(03)00183-9 , Abstract

Related entities 1. Estrogen Related Receptor 2, : 1 : 2 : 217 entities Detail

Experiments performed 13 experiments Detail

1 HNCA

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker DMX (750 MHz)

#3 Model Bruker DRX (800 MHz)

Sample #1

Temperature 310 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
1	Estrogen Related Receptor 2	[U-13C; U-15N]	0.5 mM
2	DNA Strand1		0.5 mM
3	DNA Strand2		0.5 mM
4	ZINC (II) ION		0.5 mM
5	K2SO4		20 mM
6	ZnSO4		50 mM
7	dithiothreitol (DTT)		2 mM
8	NaN3		0.001 %

Sample #2

Temperature 310 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
9	Estrogen Related Receptor 2	[U-15N]	0.5 mM
10	DNA Strand1		0.5 mM
11	DNA Strand2		0.5 mM
12	ZINC (II) ION		0.5 mM
13	K2SO4		20 mM
14	ZnSO4		50 mM
15	dithiothreitol (DTT)		2 mM
16	NaN3		0.001 %

Sample #3

Temperature 310 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
17	Estrogen Related Receptor 2	[U-15N]	0.5 mM
18	K2SO4		20 mM
19	ZnSO4		50 mM
20	dithiothreitol (DTT)		2 mM
21	NaN3		0.001 %

Sample #4

Temperature 310 (±1) K, pH 6.8 (±0.1)

#	Name	Concentration
22	DNA Strand1	0.5 mM
23	DNA Strand2	0.5 mM
24	K2SO4	20 mM
25	ZnSO4	50 mM
26	dithiothreitol (DTT)	2 mM
27	NaN3	0.001 %

2 HNCOCA

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker DMX (750 MHz)

#3 Model Bruker DRX (800 MHz)

Sample #1

Temperature 310 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
1	Estrogen Related Receptor 2	[U-13C; U-15N]	0.5 mM
2	DNA Strand1		0.5 mM
3	DNA Strand2		0.5 mM
4	ZINC (II) ION		0.5 mM
5	K2SO4		20 mM
6	ZnSO4		50 mM
7	dithiothreitol (DTT)		2 mM
8	NaN3		0.001 %

Sample #2

Temperature 310 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
9	Estrogen Related Receptor 2	[U-15N]	0.5 mM
10	DNA Strand1		0.5 mM
11	DNA Strand2		0.5 mM
12	ZINC (II) ION		0.5 mM
13	K2SO4		20 mM
14	ZnSO4		50 mM
15	dithiothreitol (DTT)		2 mM
16	NaN3		0.001 %

Sample #3

Temperature 310 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
17	Estrogen Related Receptor 2	[U-15N]	0.5 mM
18	K2SO4		20 mM
19	ZnSO4		50 mM
20	dithiothreitol (DTT)		2 mM
21	NaN3		0.001 %

Sample #4

Temperature 310 (±1) K, pH 6.8 (±0.1)

#	Name	Concentration
22	DNA Strand1	0.5 mM
23	DNA Strand2	0.5 mM
24	K2SO4	20 mM
25	ZnSO4	50 mM
26	dithiothreitol (DTT)	2 mM
27	NaN3	0.001 %

3 HNCO

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker DMX (750 MHz)

#3 Model Bruker DRX (800 MHz)

Sample #1

Temperature 310 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
1	Estrogen Related Receptor 2	[U-13C; U-15N]	0.5 mM
2	DNA Strand1		0.5 mM
3	DNA Strand2		0.5 mM
4	ZINC (II) ION		0.5 mM
5	K2SO4		20 mM
6	ZnSO4		50 mM
7	dithiothreitol (DTT)		2 mM
8	NaN3		0.001 %

Sample #2

Temperature 310 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
9	Estrogen Related Receptor 2	[U-15N]	0.5 mM
10	DNA Strand1		0.5 mM
11	DNA Strand2		0.5 mM
12	ZINC (II) ION		0.5 mM
13	K2SO4		20 mM
14	ZnSO4		50 mM
15	dithiothreitol (DTT)		2 mM
16	NaN3		0.001 %

Sample #3

Temperature 310 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
17	Estrogen Related Receptor 2	[U-15N]	0.5 mM
18	K2SO4		20 mM
19	ZnSO4		50 mM
20	dithiothreitol (DTT)		2 mM
21	NaN3		0.001 %

Sample #4

Temperature 310 (±1) K, pH 6.8 (±0.1)

#	Name	Concentration
22	DNA Strand1	0.5 mM
23	DNA Strand2	0.5 mM
24	K2SO4	20 mM
25	ZnSO4	50 mM
26	dithiothreitol (DTT)	2 mM
27	NaN3	0.001 %

4 HCACO

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker DMX (750 MHz)

#3 Model Bruker DRX (800 MHz)

Sample #1

Temperature 310 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
1	Estrogen Related Receptor 2	[U-13C; U-15N]	0.5 mM
2	DNA Strand1		0.5 mM
3	DNA Strand2		0.5 mM
4	ZINC (II) ION		0.5 mM
5	K2SO4		20 mM
6	ZnSO4		50 mM
7	dithiothreitol (DTT)		2 mM
8	NaN3		0.001 %

Sample #2

Temperature 310 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
9	Estrogen Related Receptor 2	[U-15N]	0.5 mM
10	DNA Strand1		0.5 mM
11	DNA Strand2		0.5 mM
12	ZINC (II) ION		0.5 mM
13	K2SO4		20 mM
14	ZnSO4		50 mM
15	dithiothreitol (DTT)		2 mM
16	NaN3		0.001 %

Sample #3

Temperature 310 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
17	Estrogen Related Receptor 2	[U-15N]	0.5 mM
18	K2SO4		20 mM
19	ZnSO4		50 mM
20	dithiothreitol (DTT)		2 mM
21	NaN3		0.001 %

Sample #4

Temperature 310 (±1) K, pH 6.8 (±0.1)

#	Name	Concentration
22	DNA Strand1	0.5 mM
23	DNA Strand2	0.5 mM
24	K2SO4	20 mM
25	ZnSO4	50 mM
26	dithiothreitol (DTT)	2 mM
27	NaN3	0.001 %

5 (H)CCH-COSY

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker DMX (750 MHz)

#3 Model Bruker DRX (800 MHz)

Sample #1

Temperature 310 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
1	Estrogen Related Receptor 2	[U-13C; U-15N]	0.5 mM
2	DNA Strand1		0.5 mM
3	DNA Strand2		0.5 mM
4	ZINC (II) ION		0.5 mM
5	K2SO4		20 mM
6	ZnSO4		50 mM
7	dithiothreitol (DTT)		2 mM
8	NaN3		0.001 %

Sample #2

Temperature 310 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
9	Estrogen Related Receptor 2	[U-15N]	0.5 mM
10	DNA Strand1		0.5 mM
11	DNA Strand2		0.5 mM
12	ZINC (II) ION		0.5 mM
13	K2SO4		20 mM
14	ZnSO4		50 mM
15	dithiothreitol (DTT)		2 mM
16	NaN3		0.001 %

Sample #3

Temperature 310 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
17	Estrogen Related Receptor 2	[U-15N]	0.5 mM
18	K2SO4		20 mM
19	ZnSO4		50 mM
20	dithiothreitol (DTT)		2 mM
21	NaN3		0.001 %

Sample #4

Temperature 310 (±1) K, pH 6.8 (±0.1)

#	Name	Concentration
22	DNA Strand1	0.5 mM
23	DNA Strand2	0.5 mM
24	K2SO4	20 mM
25	ZnSO4	50 mM
26	dithiothreitol (DTT)	2 mM
27	NaN3	0.001 %

6 (H)CCH TOCSY

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker DMX (750 MHz)

#3 Model Bruker DRX (800 MHz)

Sample #1

Temperature 310 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
1	Estrogen Related Receptor 2	[U-13C; U-15N]	0.5 mM
2	DNA Strand1		0.5 mM
3	DNA Strand2		0.5 mM
4	ZINC (II) ION		0.5 mM
5	K2SO4		20 mM
6	ZnSO4		50 mM
7	dithiothreitol (DTT)		2 mM
8	NaN3		0.001 %

Sample #2

Temperature 310 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
9	Estrogen Related Receptor 2	[U-15N]	0.5 mM
10	DNA Strand1		0.5 mM
11	DNA Strand2		0.5 mM
12	ZINC (II) ION		0.5 mM
13	K2SO4		20 mM
14	ZnSO4		50 mM
15	dithiothreitol (DTT)		2 mM
16	NaN3		0.001 %

Sample #3

Temperature 310 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
17	Estrogen Related Receptor 2	[U-15N]	0.5 mM
18	K2SO4		20 mM
19	ZnSO4		50 mM
20	dithiothreitol (DTT)		2 mM
21	NaN3		0.001 %

Sample #4

Temperature 310 (±1) K, pH 6.8 (±0.1)

#	Name	Concentration
22	DNA Strand1	0.5 mM
23	DNA Strand2	0.5 mM
24	K2SO4	20 mM
25	ZnSO4	50 mM
26	dithiothreitol (DTT)	2 mM
27	NaN3	0.001 %

7 HNHA

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker DMX (750 MHz)

#3 Model Bruker DRX (800 MHz)

Sample #1

Temperature 310 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
1	Estrogen Related Receptor 2	[U-13C; U-15N]	0.5 mM
2	DNA Strand1		0.5 mM
3	DNA Strand2		0.5 mM
4	ZINC (II) ION		0.5 mM
5	K2SO4		20 mM
6	ZnSO4		50 mM
7	dithiothreitol (DTT)		2 mM
8	NaN3		0.001 %

Sample #2

Temperature 310 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
9	Estrogen Related Receptor 2	[U-15N]	0.5 mM
10	DNA Strand1		0.5 mM
11	DNA Strand2		0.5 mM
12	ZINC (II) ION		0.5 mM
13	K2SO4		20 mM
14	ZnSO4		50 mM
15	dithiothreitol (DTT)		2 mM
16	NaN3		0.001 %

Sample #3

Temperature 310 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
17	Estrogen Related Receptor 2	[U-15N]	0.5 mM
18	K2SO4		20 mM
19	ZnSO4		50 mM
20	dithiothreitol (DTT)		2 mM
21	NaN3		0.001 %

Sample #4

Temperature 310 (±1) K, pH 6.8 (±0.1)

#	Name	Concentration
22	DNA Strand1	0.5 mM
23	DNA Strand2	0.5 mM
24	K2SO4	20 mM
25	ZnSO4	50 mM
26	dithiothreitol (DTT)	2 mM
27	NaN3	0.001 %

8 HNHB

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker DMX (750 MHz)

#3 Model Bruker DRX (800 MHz)

Sample #1

Temperature 310 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
1	Estrogen Related Receptor 2	[U-13C; U-15N]	0.5 mM
2	DNA Strand1		0.5 mM
3	DNA Strand2		0.5 mM
4	ZINC (II) ION		0.5 mM
5	K2SO4		20 mM
6	ZnSO4		50 mM
7	dithiothreitol (DTT)		2 mM
8	NaN3		0.001 %

Sample #2

Temperature 310 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
9	Estrogen Related Receptor 2	[U-15N]	0.5 mM
10	DNA Strand1		0.5 mM
11	DNA Strand2		0.5 mM
12	ZINC (II) ION		0.5 mM
13	K2SO4		20 mM
14	ZnSO4		50 mM
15	dithiothreitol (DTT)		2 mM
16	NaN3		0.001 %

Sample #3

Temperature 310 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
17	Estrogen Related Receptor 2	[U-15N]	0.5 mM
18	K2SO4		20 mM
19	ZnSO4		50 mM
20	dithiothreitol (DTT)		2 mM
21	NaN3		0.001 %

Sample #4

Temperature 310 (±1) K, pH 6.8 (±0.1)

#	Name	Concentration
22	DNA Strand1	0.5 mM
23	DNA Strand2	0.5 mM
24	K2SO4	20 mM
25	ZnSO4	50 mM
26	dithiothreitol (DTT)	2 mM
27	NaN3	0.001 %

9 HA(CA)HB-COSY

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker DMX (750 MHz)

#3 Model Bruker DRX (800 MHz)

Sample #1

Temperature 310 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
1	Estrogen Related Receptor 2	[U-13C; U-15N]	0.5 mM
2	DNA Strand1		0.5 mM
3	DNA Strand2		0.5 mM
4	ZINC (II) ION		0.5 mM
5	K2SO4		20 mM
6	ZnSO4		50 mM
7	dithiothreitol (DTT)		2 mM
8	NaN3		0.001 %

Sample #2

Temperature 310 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
9	Estrogen Related Receptor 2	[U-15N]	0.5 mM
10	DNA Strand1		0.5 mM
11	DNA Strand2		0.5 mM
12	ZINC (II) ION		0.5 mM
13	K2SO4		20 mM
14	ZnSO4		50 mM
15	dithiothreitol (DTT)		2 mM
16	NaN3		0.001 %

Sample #3

Temperature 310 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
17	Estrogen Related Receptor 2	[U-15N]	0.5 mM
18	K2SO4		20 mM
19	ZnSO4		50 mM
20	dithiothreitol (DTT)		2 mM
21	NaN3		0.001 %

Sample #4

Temperature 310 (±1) K, pH 6.8 (±0.1)

#	Name	Concentration
22	DNA Strand1	0.5 mM
23	DNA Strand2	0.5 mM
24	K2SO4	20 mM
25	ZnSO4	50 mM
26	dithiothreitol (DTT)	2 mM
27	NaN3	0.001 %

10 2D [13C,15N ] u2-filtered TOCSY

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker DMX (750 MHz)

#3 Model Bruker DRX (800 MHz)

Sample #1

Temperature 310 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
1	Estrogen Related Receptor 2	[U-13C; U-15N]	0.5 mM
2	DNA Strand1		0.5 mM
3	DNA Strand2		0.5 mM
4	ZINC (II) ION		0.5 mM
5	K2SO4		20 mM
6	ZnSO4		50 mM
7	dithiothreitol (DTT)		2 mM
8	NaN3		0.001 %

Sample #2

Temperature 310 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
9	Estrogen Related Receptor 2	[U-15N]	0.5 mM
10	DNA Strand1		0.5 mM
11	DNA Strand2		0.5 mM
12	ZINC (II) ION		0.5 mM
13	K2SO4		20 mM
14	ZnSO4		50 mM
15	dithiothreitol (DTT)		2 mM
16	NaN3		0.001 %

Sample #3

Temperature 310 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
17	Estrogen Related Receptor 2	[U-15N]	0.5 mM
18	K2SO4		20 mM
19	ZnSO4		50 mM
20	dithiothreitol (DTT)		2 mM
21	NaN3		0.001 %

Sample #4

Temperature 310 (±1) K, pH 6.8 (±0.1)

#	Name	Concentration
22	DNA Strand1	0.5 mM
23	DNA Strand2	0.5 mM
24	K2SO4	20 mM
25	ZnSO4	50 mM
26	dithiothreitol (DTT)	2 mM
27	NaN3	0.001 %

11 2D [13C,15N] u1,u2-filtered NOESY

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker DMX (750 MHz)

#3 Model Bruker DRX (800 MHz)

Sample #1

Temperature 310 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
1	Estrogen Related Receptor 2	[U-13C; U-15N]	0.5 mM
2	DNA Strand1		0.5 mM
3	DNA Strand2		0.5 mM
4	ZINC (II) ION		0.5 mM
5	K2SO4		20 mM
6	ZnSO4		50 mM
7	dithiothreitol (DTT)		2 mM
8	NaN3		0.001 %

Sample #2

Temperature 310 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
9	Estrogen Related Receptor 2	[U-15N]	0.5 mM
10	DNA Strand1		0.5 mM
11	DNA Strand2		0.5 mM
12	ZINC (II) ION		0.5 mM
13	K2SO4		20 mM
14	ZnSO4		50 mM
15	dithiothreitol (DTT)		2 mM
16	NaN3		0.001 %

Sample #3

Temperature 310 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
17	Estrogen Related Receptor 2	[U-15N]	0.5 mM
18	K2SO4		20 mM
19	ZnSO4		50 mM
20	dithiothreitol (DTT)		2 mM
21	NaN3		0.001 %

Sample #4

Temperature 310 (±1) K, pH 6.8 (±0.1)

#	Name	Concentration
22	DNA Strand1	0.5 mM
23	DNA Strand2	0.5 mM
24	K2SO4	20 mM
25	ZnSO4	50 mM
26	dithiothreitol (DTT)	2 mM
27	NaN3	0.001 %

12 3D 15N separated NOESY

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker DMX (750 MHz)

#3 Model Bruker DRX (800 MHz)

Sample #1

Temperature 310 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
1	Estrogen Related Receptor 2	[U-13C; U-15N]	0.5 mM
2	DNA Strand1		0.5 mM
3	DNA Strand2		0.5 mM
4	ZINC (II) ION		0.5 mM
5	K2SO4		20 mM
6	ZnSO4		50 mM
7	dithiothreitol (DTT)		2 mM
8	NaN3		0.001 %

Sample #2

Temperature 310 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
9	Estrogen Related Receptor 2	[U-15N]	0.5 mM
10	DNA Strand1		0.5 mM
11	DNA Strand2		0.5 mM
12	ZINC (II) ION		0.5 mM
13	K2SO4		20 mM
14	ZnSO4		50 mM
15	dithiothreitol (DTT)		2 mM
16	NaN3		0.001 %

Sample #3

Temperature 310 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
17	Estrogen Related Receptor 2	[U-15N]	0.5 mM
18	K2SO4		20 mM
19	ZnSO4		50 mM
20	dithiothreitol (DTT)		2 mM
21	NaN3		0.001 %

Sample #4

Temperature 310 (±1) K, pH 6.8 (±0.1)

#	Name	Concentration
22	DNA Strand1	0.5 mM
23	DNA Strand2	0.5 mM
24	K2SO4	20 mM
25	ZnSO4	50 mM
26	dithiothreitol (DTT)	2 mM
27	NaN3	0.001 %

13 3D 13C-separated NOESY

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker DMX (750 MHz)

#3 Model Bruker DRX (800 MHz)

Sample #1

Temperature 310 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
1	Estrogen Related Receptor 2	[U-13C; U-15N]	0.5 mM
2	DNA Strand1		0.5 mM
3	DNA Strand2		0.5 mM
4	ZINC (II) ION		0.5 mM
5	K2SO4		20 mM
6	ZnSO4		50 mM
7	dithiothreitol (DTT)		2 mM
8	NaN3		0.001 %

Sample #2

Temperature 310 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
9	Estrogen Related Receptor 2	[U-15N]	0.5 mM
10	DNA Strand1		0.5 mM
11	DNA Strand2		0.5 mM
12	ZINC (II) ION		0.5 mM
13	K2SO4		20 mM
14	ZnSO4		50 mM
15	dithiothreitol (DTT)		2 mM
16	NaN3		0.001 %

Sample #3

Temperature 310 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Concentration
17	Estrogen Related Receptor 2	[U-15N]	0.5 mM
18	K2SO4		20 mM
19	ZnSO4		50 mM
20	dithiothreitol (DTT)		2 mM
21	NaN3		0.001 %

Sample #4

Temperature 310 (±1) K, pH 6.8 (±0.1)

#	Name	Concentration
22	DNA Strand1	0.5 mM
23	DNA Strand2	0.5 mM
24	K2SO4	20 mM
25	ZnSO4	50 mM
26	dithiothreitol (DTT)	2 mM
27	NaN3	0.001 %

nullKeywords ERR2, Hormone Nuclear Receptor

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BMRB: 5363

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Chemical shift reference

Chemical shift reference

Related entities 1. Estrogen Related Receptor 2, : 1 : 2 : 217 entities Detail

Experiments performed 13 experiments Detail

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