BMRB: 5364

Structure of the C-terminal FG-binding domain of human Tap

Authors

Grant, R.P., Neuhaus, D., Stewart, M.

Assembly

Tap

Entity

1. Tap (polymer, Thiol state: all free), 69 monomers, 7712.545 Da Detail

PAPTPSSSPV PTLSPEQQEM LQAFSTQSGM NLEWSQKCLQ DNNWDYTRSA QAFTHLKAKG EIPEVAFMK

Formula weight

7712.545 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

SIMULATED ANNEALING

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.7 %, Completeness: 93.4 %, Completeness (bb): 97.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	93.4 % (396 of 424)	93.4 % (396 of 424)
Backbone	97.7 % (130 of 133)	97.7 % (130 of 133)
Sidechain	91.4 % (266 of 291)	91.4 % (266 of 291)
Aromatic	100.0 % (33 of 33)	100.0 % (33 of 33)
Methyl	100.0 % (27 of 27)	100.0 % (27 of 27)

1. Tap C-terminal domain

PAPTPSSSPV PTLSPEQQEM LQAFSTQSGM NLEWSQKCLQ DNNWDYTRSA QAFTHLKAKG EIPEVAFMK

Show sample condition

Sample

Temperature 300 (±0.5) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Tap C-terminal domain			3 mM
2	potassium phosphate			25 mM
3	sodium chloride			50 mM
4	deuterium oxide			10 %
5	sodium trimethylsilyl propionate			0.1 mM

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Protein Blocks Logo

Calculated from 26 models in PDB: 1GO5, Strand ID: A Detail

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Release date

2002-05-29

Citation

Structure of the C-terminal FG-nucleoporin binding domain of Tap/NXF1
Grant, R.P., Hurt, E., Neuhaus, D., Stewart, M.
Nat. Struct. Biol. (2002), 9, 247-251, PubMed 11875519 , DOI 10.1038/nsb773 , Abstract

Related entities 1. Tap, : 1 : 7 : 3 : 34 entities Detail

Interaction partners 1. Tap, : 85 interactors Detail

Experiments performed 4 experiments Detail