BMRB: 5366

SOLUTION STRUCTURE OF THE VAM7P PX DOMAIN

Authors

Lu, J., Garcia, Dulubova, I., Sudhof, T.C., Rizo, J.

Assembly

Vacuolar morphogenesis protein

Entity

1. Vacuolar morphogenesis protein (polymer, Thiol state: not present), 124 monomers, 15264.34 Da Detail

KMSEKLRKMS EKLRIKVDDV KINPKYVLYG VSTPNKRLYK RYSEFWKLKT RLERDVGSTI PYDFPEKPGV LDRRWQRRYD DPEMIDERRI GLERFLNELY NDRFDSRWRD TKIAQDFLQL SKPN

Formula weight

15264.34 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.4 %, Completeness: 91.2 %, Completeness (bb): 92.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	91.2 % (1455 of 1596)	90.3 % (773 of 856)	92.2 % (564 of 612)	92.2 % (118 of 128)
Backbone	92.1 % (672 of 730)	91.8 % (225 of 245)	92.4 % (340 of 368)	91.5 % (107 of 117)
Sidechain	90.6 % (893 of 986)	89.7 % (548 of 611)	91.8 % (334 of 364)	100.0 % (11 of 11)
Aromatic	100.0 % (142 of 142)	100.0 % (71 of 71)	100.0 % (68 of 68)	100.0 % (3 of 3)
Methyl	92.5 % (98 of 106)	92.5 % (49 of 53)	92.5 % (49 of 53)

1. vam7p

KMSEKLRKMS EKLRIKVDDV KINPKYVLYG VSTPNKRLYK RYSEFWKLKT RLERDVGSTI PYDFPEKPGV LDRRWQRRYD DPEMIDERRI GLERFLNELY NDRFDSRWRD TKIAQDFLQL SKPN

Show sample condition

Sample

Pressure 1 atm, Temperature 298 (±1) K, pH 6.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	vam7p	[U-15N]		0.5 mM
2	Mes buffer			20 mM
3	H2O			90 %
4	D2O			10 %

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LACS Plot; CA

Referencing offset: -0.44 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.44 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.04 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 2.07 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 1KMD, Strand ID: A Detail

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Release date

2003-02-24

Citation

Solution structure of the Vam7p PX domain
Lu, J., Garcia, J.J., Dulubova, I., Sudhof, T.C., Rizo, J.
Biochemistry (2002), 41, 5956-5962, PubMed 11993989 , DOI: , Abstract

Related entities 1. Vacuolar morphogenesis protein, : 1 : 9 entities Detail

Interaction partners 1. Vacuolar morphogenesis protein, : 39 interactors Detail

Experiments performed 2 experiments Detail