BMRB: 5371

U6 RNA chemical shifts

Authors

Butcher, S.E.

Assembly

U6 RNA

Entity

1. U6 RNA (polymer, Thiol state: not present), 24 monomers, 7731.585 Da Detail

GGUUCCCCUG CAUAAGGAUG AACC

Formula weight

7731.585 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 65.1 %, Completeness (bb): 53.4 % Detail

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Polymer type: polyribonucleotide

	Total	1H	13C	15N
All	65.1 % (250 of 384)	94.8 % (200 of 211)	25.3 % (41 of 162)	81.8 % (9 of 11)
Suger, PO4	53.4 % (141 of 264)	97.9 % (141 of 144)	0.0 % (0 of 120)
Nucleobase	90.8 % (109 of 120)	88.1 % (59 of 67)	97.6 % (41 of 42)	81.8 % (9 of 11)
Aromatic	95.3 % (101 of 106)	96.2 % (51 of 53)	97.6 % (41 of 42)	81.8 % (9 of 11)

1. U6 RNA

GGUUCCCCUG CAUAAGGAUG AACC

Show sample condition

Sample

Temperature 303 (±0.5) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	U6 RNA	[U-100% 13C; U-15N]		1.0 mM

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Release date

2002-08-21

Citation

Metal binding and base ionization in the U6 RNA intramolecular stem-loop structure
Huppler, A.E., Nikstad, L.J., Allmann, A.M., Brow, D.A., Butcher, S.E.
Nat. Struct. Biol. (2002), 9, 431-435, PubMed 11992125 , DOI 10.1038/nsb800 , Abstract

Related entities 1. U6 RNA, : 1 : 5 : 4 : 1 entities Detail

Experiments performed 1 experiments Detail