BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	95.2 % (1266 of 1330)	96.3 % (669 of 695)	93.9 % (480 of 511)	94.4 % (117 of 124)
Backbone	95.8 % (642 of 670)	96.9 % (219 of 226)	95.2 % (319 of 335)	95.4 % (104 of 109)
Sidechain	95.1 % (731 of 769)	95.9 % (450 of 469)	94.0 % (268 of 285)	86.7 % (13 of 15)
Aromatic	96.7 % (58 of 60)	100.0 % (30 of 30)	96.6 % (28 of 29)	0.0 % (0 of 1)
Methyl	97.1 % (134 of 138)	100.0 % (69 of 69)	94.2 % (65 of 69)

1. cold shock response protein Yfia

MTMNITSKQM EITPAIRQHV ADRLAKLEKW QTHLINPHII LSKEPQGFVA DATINTPNGV LVASGKHEDM YTAINELINK LERQLNKLQH KGEARRAATS VKDANFVEEV EEE

Show sample condition

Sample

Temperature 300 (±0.2) K, pH 6.65 (±0.15)

#	Name	Isotope labeling	Type	Concentration
1	cold shock response protein Yfia	[U-13C; U-15N]		0.5 ~ 0.8 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.13 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.13 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.06 ppm, Outliers: 3 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.46 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 29 models in PDB: 1N3G, Strand ID: A Detail

Release date

2002-09-24

Citation

1H, 13C and 15N resonance assignments of the ribosome-associated cold shock response protein Yfia of Escherichia coli
Kalinin, A., Rak, A., Shcherbakov, D., Bayer, P.
J. Biomol. NMR (2002), 23, 335-336, PubMed 12398359 , DOI: , Abstract

Related entities 1. Yfia, : 1 : 24 : 4 : 30 entities Detail

Experiments performed 14 experiments Detail

1 3D-HNCA

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Varian INOVA (600 MHz)

Sample #1

Temperature 300 (±0.2) K, pH 6.65 (±0.15)

#	Name	Isotope labeling	Type	Concentration
1	cold shock response protein Yfia	[U-13C; U-15N]		0.5 ~ 0.8 mM

Sample #2

Temperature 300 (±0.2) K, pH 6.65 (±0.15)

#	Name	Isotope labeling	Type	Concentration
2	cold shock response protein Yfia			0.5 ~ 0.8 mM

2 3D-HNCO

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Varian INOVA (600 MHz)

Sample #1

Temperature 300 (±0.2) K, pH 6.65 (±0.15)

#	Name	Isotope labeling	Type	Concentration
1	cold shock response protein Yfia	[U-13C; U-15N]		0.5 ~ 0.8 mM

Sample #2

Temperature 300 (±0.2) K, pH 6.65 (±0.15)

#	Name	Isotope labeling	Type	Concentration
2	cold shock response protein Yfia			0.5 ~ 0.8 mM

3 3D-HNCACB

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Varian INOVA (600 MHz)

Sample #1

Temperature 300 (±0.2) K, pH 6.65 (±0.15)

#	Name	Isotope labeling	Type	Concentration
1	cold shock response protein Yfia	[U-13C; U-15N]		0.5 ~ 0.8 mM

Sample #2

Temperature 300 (±0.2) K, pH 6.65 (±0.15)

#	Name	Isotope labeling	Type	Concentration
2	cold shock response protein Yfia			0.5 ~ 0.8 mM

4 3D-HC(CO)NH

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Varian INOVA (600 MHz)

Sample #1

Temperature 300 (±0.2) K, pH 6.65 (±0.15)

#	Name	Isotope labeling	Type	Concentration
1	cold shock response protein Yfia	[U-13C; U-15N]		0.5 ~ 0.8 mM

Sample #2

Temperature 300 (±0.2) K, pH 6.65 (±0.15)

#	Name	Isotope labeling	Type	Concentration
2	cold shock response protein Yfia			0.5 ~ 0.8 mM

5 3D-C(CO)NH

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Varian INOVA (600 MHz)

Sample #1

Temperature 300 (±0.2) K, pH 6.65 (±0.15)

#	Name	Isotope labeling	Type	Concentration
1	cold shock response protein Yfia	[U-13C; U-15N]		0.5 ~ 0.8 mM

Sample #2

Temperature 300 (±0.2) K, pH 6.65 (±0.15)

#	Name	Isotope labeling	Type	Concentration
2	cold shock response protein Yfia			0.5 ~ 0.8 mM

6 3D-HCCH-TOCSY

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Varian INOVA (600 MHz)

Sample #1

Temperature 300 (±0.2) K, pH 6.65 (±0.15)

#	Name	Isotope labeling	Type	Concentration
1	cold shock response protein Yfia	[U-13C; U-15N]		0.5 ~ 0.8 mM

Sample #2

Temperature 300 (±0.2) K, pH 6.65 (±0.15)

#	Name	Isotope labeling	Type	Concentration
2	cold shock response protein Yfia			0.5 ~ 0.8 mM

7 3D-1H,15N-HSQC-TOCSY

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Varian INOVA (600 MHz)

Sample #1

Temperature 300 (±0.2) K, pH 6.65 (±0.15)

#	Name	Isotope labeling	Type	Concentration
1	cold shock response protein Yfia	[U-13C; U-15N]		0.5 ~ 0.8 mM

Sample #2

Temperature 300 (±0.2) K, pH 6.65 (±0.15)

#	Name	Isotope labeling	Type	Concentration
2	cold shock response protein Yfia			0.5 ~ 0.8 mM

8 3D-1H,15N-HSQC-NOESY

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Varian INOVA (600 MHz)

Sample #1

Temperature 300 (±0.2) K, pH 6.65 (±0.15)

#	Name	Isotope labeling	Type	Concentration
1	cold shock response protein Yfia	[U-13C; U-15N]		0.5 ~ 0.8 mM

Sample #2

Temperature 300 (±0.2) K, pH 6.65 (±0.15)

#	Name	Isotope labeling	Type	Concentration
2	cold shock response protein Yfia			0.5 ~ 0.8 mM

9 3D-1H,13C-HSQC-NOESY

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Varian INOVA (600 MHz)

Sample #1

Temperature 300 (±0.2) K, pH 6.65 (±0.15)

#	Name	Isotope labeling	Type	Concentration
1	cold shock response protein Yfia	[U-13C; U-15N]		0.5 ~ 0.8 mM

Sample #2

Temperature 300 (±0.2) K, pH 6.65 (±0.15)

#	Name	Isotope labeling	Type	Concentration
2	cold shock response protein Yfia			0.5 ~ 0.8 mM

10 2D-1H,13C-HSQC

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Varian INOVA (600 MHz)

Sample #1

Temperature 300 (±0.2) K, pH 6.65 (±0.15)

#	Name	Isotope labeling	Type	Concentration
1	cold shock response protein Yfia	[U-13C; U-15N]		0.5 ~ 0.8 mM

Sample #2

Temperature 300 (±0.2) K, pH 6.65 (±0.15)

#	Name	Isotope labeling	Type	Concentration
2	cold shock response protein Yfia			0.5 ~ 0.8 mM

11 2D-1H,15N-HSQC

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Varian INOVA (600 MHz)

Sample #1

Temperature 300 (±0.2) K, pH 6.65 (±0.15)

#	Name	Isotope labeling	Type	Concentration
1	cold shock response protein Yfia	[U-13C; U-15N]		0.5 ~ 0.8 mM

Sample #2

Temperature 300 (±0.2) K, pH 6.65 (±0.15)

#	Name	Isotope labeling	Type	Concentration
2	cold shock response protein Yfia			0.5 ~ 0.8 mM

12 2D-1H,1H-DQF-COSY

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Varian INOVA (600 MHz)

Sample #1

Temperature 300 (±0.2) K, pH 6.65 (±0.15)

#	Name	Isotope labeling	Type	Concentration
1	cold shock response protein Yfia	[U-13C; U-15N]		0.5 ~ 0.8 mM

Sample #2

Temperature 300 (±0.2) K, pH 6.65 (±0.15)

#	Name	Isotope labeling	Type	Concentration
2	cold shock response protein Yfia			0.5 ~ 0.8 mM

13 2D-1H,1H-TOCSY

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Varian INOVA (600 MHz)

Sample #1

Temperature 300 (±0.2) K, pH 6.65 (±0.15)

#	Name	Isotope labeling	Type	Concentration
1	cold shock response protein Yfia	[U-13C; U-15N]		0.5 ~ 0.8 mM

Sample #2

Temperature 300 (±0.2) K, pH 6.65 (±0.15)

#	Name	Isotope labeling	Type	Concentration
2	cold shock response protein Yfia			0.5 ~ 0.8 mM

14 2D-1H,1H-NOESY

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Varian INOVA (600 MHz)

Sample #1

Temperature 300 (±0.2) K, pH 6.65 (±0.15)

#	Name	Isotope labeling	Type	Concentration
1	cold shock response protein Yfia	[U-13C; U-15N]		0.5 ~ 0.8 mM

Sample #2

Temperature 300 (±0.2) K, pH 6.65 (±0.15)

#	Name	Isotope labeling	Type	Concentration
2	cold shock response protein Yfia			0.5 ~ 0.8 mM

nullKeywords cold shock protein

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Chemical shift reference

Chemical shift reference

Related entities 1. Yfia, : 1 : 24 : 4 : 30 entities Detail

Experiments performed 14 experiments Detail

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