BMRB: 5392

Solution structure of HMG box 5 in human upstream binding factor

Authors

Yang, W., Xu, Y., Wu, J., Zeng, W., Shi, Y.

Assembly

Upstream binding factor 1

Entity

1. Upstream binding factor 1 (polymer, Thiol state: not present), 84 monomers, 9912.342 Da Detail

GKLPESPKRA EEIWQQSVIG DYLARFKNDR VKALKAMEMT WNNMEKKEKL MWIKKAAEDQ KRYERELSEM RAPPAATNSS KKLE

Formula weight

9912.342 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 88.2 %, Completeness (bb): 98.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	88.2 % (923 of 1046)	85.1 % (478 of 562)	90.4 % (356 of 394)	98.9 % (89 of 90)
Backbone	98.4 % (488 of 496)	98.8 % (164 of 166)	98.0 % (245 of 250)	98.8 % (79 of 80)
Sidechain	81.8 % (517 of 632)	79.3 % (314 of 396)	85.4 % (193 of 226)	100.0 % (10 of 10)
Aromatic	53.2 % (33 of 62)	96.8 % (30 of 31)	0.0 % (0 of 28)	100.0 % (3 of 3)
Methyl	100.0 % (66 of 66)	100.0 % (33 of 33)	100.0 % (33 of 33)

1. UBF1

GKLPESPKRA EEIWQQSVIG DYLARFKNDR VKALKAMEMT WNNMEKKEKL MWIKKAAEDQ KRYERELSEM RAPPAATNSS KKLE

Show sample condition

Sample

Pressure 1 atm, Temperature 300 K, pH 5.5

#	Name	Isotope labeling	Type	Concentration
1	UBF1	[U-15N; U-13C]		3 mM
2	NaPO4			45 mM
3	H20			90 %
4	D20			10 %

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LACS Plot; CA

Referencing offset: 0.08 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	H2O	methyl protons	4.73 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CB

Referencing offset: 0.08 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	H2O	methyl protons	4.73 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; HA

Referencing offset: 0.11 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	H2O	methyl protons	4.73 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CO

Referencing offset: -0.16 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	H2O	methyl protons	4.73 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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Protein Blocks Logo

Calculated from 30 models in PDB: 1L8Y, Strand ID: A Detail

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Release date

2003-12-07

Citation

Solution structure and DNA binding property of the fifth HMG box domain in comparison with the first HMG box domain in human upstream binding factor
Yang, W., Xu, Y., Wu, J., Zeng, W., Shi, Y.
Biochemistry (2003), 42, 1930-1938, PubMed 12590579 , DOI 10.1021/bi026372x , Abstract

Related entities 1. Upstream binding factor 1, : 1 : 2 : 13 entities Detail

Interaction partners 1. Upstream binding factor 1, : 29 interactors Detail

Experiments performed 2 experiments Detail