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BMRB: 5398

1LMZ

Backbone and side chain 1H, 13C, and 15N chemical shift assignments for 3-methyladenine DNA glycosylase I (TAG) from Escherichia coli

Authors

Stivers, J.T., Drohat, A.C.

Assembly

3-methyladenine DNA glycosylase I

Entity

1. 3-methyladenine DNA glycosylase I (polymer, Thiol state: all free), 187 monomers, 21113.91 Da Detail

MERCGWVSQD PLYIAYHDNE WGVPETDSKK LFEMICLEGQ QAGLSWITVL KKRENYRACF HQFDPVKVAA MQEEDVERLV QDAGIIRHRG KIQAIIGNAR AYLQMEQNGE PFADFVWSFV NHQPQMTQAT TLSEIPTSTP ASDALSKALK KRGFKFVGTT ICYSFMQACG LVNDHVVGCC CYPGNKP

Formula weight

21113.91 Da

Source organism

Escherichia coli

Exptl. method

NMR

Refine. method

Structures were calculated from an extended strand using CNS 1.1,

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.5 %, Completeness: 90.9 %, Completeness (bb): 97.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	90.9 % (1991 of 2191)	90.7 % (1034 of 1140)	91.9 % (780 of 849)	87.6 % (177 of 202)
Backbone	97.5 % (1076 of 1104)	96.8 % (366 of 378)	98.2 % (538 of 548)	96.6 % (172 of 178)
Sidechain	86.0 % (1085 of 1261)	87.7 % (668 of 762)	86.7 % (412 of 475)	20.8 % (5 of 24)
Aromatic	71.4 % (147 of 206)	77.7 % (80 of 103)	64.6 % (64 of 99)	75.0 % (3 of 4)
Methyl	98.9 % (182 of 184)	97.8 % (90 of 92)	100.0 % (92 of 92)

1. 3-methyladenine DNA glycosylase I

Show sample condition

Sample

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
11	3-methyladenine DNA glycosylase I	[U-13C; U-15N]	0.5 ~ 1.0 mM
12	sodium phosphate buffer		10 mM
13	NaCl		100 mM
14	DTT		3 mM
15	NaN3		0.3 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.29 ppm, Outliers: 6 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.29 ppm, Outliers: 6 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.03 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.2 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 25 models in PDB: 1LMZ, Strand ID: A Detail

Release date

2003-02-19

Citation

3-Methyladenine DNA glycosylase I is an unexpected helix-hairpin-helix superfamily member
Drohat, A.C., Kwon, K., Krosky, D.J., Stivers, J.T.
Nat. Struct. Biol. (2002), 9, 659-664, PubMed 12161745 , DOI 10.1038/nsb829 , Abstract

Related entities 1. 3-methyladenine DNA glycosylase I, : 1 : 2 : 47 entities Detail

Interaction partners 1. 3-methyladenine DNA glycosylase I, : 3 interactors Detail

More details for 3 interactors identified by 2 citations

Experiments performed 17 experiments Detail

1 13C-edited HSQC

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DMX (750 MHz)

#4 Model Varian UnityPlus (600 MHz)

Sample #1

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Concentration
1	3-methyladenine DNA glycosylase I	0.5 ~ 1.0 mM
2	sodium phosphate buffer	10 mM
3	NaCl	100 mM
4	DTT	3 mM
5	NaN3	0.3 mM

Sample #2

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
6	3-methyladenine DNA glycosylase I	[U-15N]	0.5 ~ 1.0 mM
7	sodium phosphate buffer		10 mM
8	NaCl		100 mM
9	DTT		3 mM
10	NaN3		0.3 mM

Sample #3

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
11	3-methyladenine DNA glycosylase I	[U-13C; U-15N]	0.5 ~ 1.0 mM
12	sodium phosphate buffer		10 mM
13	NaCl		100 mM
14	DTT		3 mM
15	NaN3		0.3 mM

2 15N-edited HSQC

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DMX (750 MHz)

#4 Model Varian UnityPlus (600 MHz)

Sample #1

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Concentration
1	3-methyladenine DNA glycosylase I	0.5 ~ 1.0 mM
2	sodium phosphate buffer	10 mM
3	NaCl	100 mM
4	DTT	3 mM
5	NaN3	0.3 mM

Sample #2

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
6	3-methyladenine DNA glycosylase I	[U-15N]	0.5 ~ 1.0 mM
7	sodium phosphate buffer		10 mM
8	NaCl		100 mM
9	DTT		3 mM
10	NaN3		0.3 mM

Sample #3

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
11	3-methyladenine DNA glycosylase I	[U-13C; U-15N]	0.5 ~ 1.0 mM
12	sodium phosphate buffer		10 mM
13	NaCl		100 mM
14	DTT		3 mM
15	NaN3		0.3 mM

3 2D NOESY (t = 125 ms) in D2O

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DMX (750 MHz)

#4 Model Varian UnityPlus (600 MHz)

Sample #1

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Concentration
1	3-methyladenine DNA glycosylase I	0.5 ~ 1.0 mM
2	sodium phosphate buffer	10 mM
3	NaCl	100 mM
4	DTT	3 mM
5	NaN3	0.3 mM

Sample #2

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
6	3-methyladenine DNA glycosylase I	[U-15N]	0.5 ~ 1.0 mM
7	sodium phosphate buffer		10 mM
8	NaCl		100 mM
9	DTT		3 mM
10	NaN3		0.3 mM

Sample #3

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
11	3-methyladenine DNA glycosylase I	[U-13C; U-15N]	0.5 ~ 1.0 mM
12	sodium phosphate buffer		10 mM
13	NaCl		100 mM
14	DTT		3 mM
15	NaN3		0.3 mM

4 2D TOCSY in D2O

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DMX (750 MHz)

#4 Model Varian UnityPlus (600 MHz)

Sample #1

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Concentration
1	3-methyladenine DNA glycosylase I	0.5 ~ 1.0 mM
2	sodium phosphate buffer	10 mM
3	NaCl	100 mM
4	DTT	3 mM
5	NaN3	0.3 mM

Sample #2

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
6	3-methyladenine DNA glycosylase I	[U-15N]	0.5 ~ 1.0 mM
7	sodium phosphate buffer		10 mM
8	NaCl		100 mM
9	DTT		3 mM
10	NaN3		0.3 mM

Sample #3

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
11	3-methyladenine DNA glycosylase I	[U-13C; U-15N]	0.5 ~ 1.0 mM
12	sodium phosphate buffer		10 mM
13	NaCl		100 mM
14	DTT		3 mM
15	NaN3		0.3 mM

5 HNCA

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DMX (750 MHz)

#4 Model Varian UnityPlus (600 MHz)

Sample #1

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Concentration
1	3-methyladenine DNA glycosylase I	0.5 ~ 1.0 mM
2	sodium phosphate buffer	10 mM
3	NaCl	100 mM
4	DTT	3 mM
5	NaN3	0.3 mM

Sample #2

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
6	3-methyladenine DNA glycosylase I	[U-15N]	0.5 ~ 1.0 mM
7	sodium phosphate buffer		10 mM
8	NaCl		100 mM
9	DTT		3 mM
10	NaN3		0.3 mM

Sample #3

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
11	3-methyladenine DNA glycosylase I	[U-13C; U-15N]	0.5 ~ 1.0 mM
12	sodium phosphate buffer		10 mM
13	NaCl		100 mM
14	DTT		3 mM
15	NaN3		0.3 mM

6 HNCO

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DMX (750 MHz)

#4 Model Varian UnityPlus (600 MHz)

Sample #1

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Concentration
1	3-methyladenine DNA glycosylase I	0.5 ~ 1.0 mM
2	sodium phosphate buffer	10 mM
3	NaCl	100 mM
4	DTT	3 mM
5	NaN3	0.3 mM

Sample #2

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
6	3-methyladenine DNA glycosylase I	[U-15N]	0.5 ~ 1.0 mM
7	sodium phosphate buffer		10 mM
8	NaCl		100 mM
9	DTT		3 mM
10	NaN3		0.3 mM

Sample #3

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
11	3-methyladenine DNA glycosylase I	[U-13C; U-15N]	0.5 ~ 1.0 mM
12	sodium phosphate buffer		10 mM
13	NaCl		100 mM
14	DTT		3 mM
15	NaN3		0.3 mM

7 HCACO

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DMX (750 MHz)

#4 Model Varian UnityPlus (600 MHz)

Sample #1

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Concentration
1	3-methyladenine DNA glycosylase I	0.5 ~ 1.0 mM
2	sodium phosphate buffer	10 mM
3	NaCl	100 mM
4	DTT	3 mM
5	NaN3	0.3 mM

Sample #2

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
6	3-methyladenine DNA glycosylase I	[U-15N]	0.5 ~ 1.0 mM
7	sodium phosphate buffer		10 mM
8	NaCl		100 mM
9	DTT		3 mM
10	NaN3		0.3 mM

Sample #3

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
11	3-methyladenine DNA glycosylase I	[U-13C; U-15N]	0.5 ~ 1.0 mM
12	sodium phosphate buffer		10 mM
13	NaCl		100 mM
14	DTT		3 mM
15	NaN3		0.3 mM

8 HNCACB

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DMX (750 MHz)

#4 Model Varian UnityPlus (600 MHz)

Sample #1

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Concentration
1	3-methyladenine DNA glycosylase I	0.5 ~ 1.0 mM
2	sodium phosphate buffer	10 mM
3	NaCl	100 mM
4	DTT	3 mM
5	NaN3	0.3 mM

Sample #2

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
6	3-methyladenine DNA glycosylase I	[U-15N]	0.5 ~ 1.0 mM
7	sodium phosphate buffer		10 mM
8	NaCl		100 mM
9	DTT		3 mM
10	NaN3		0.3 mM

Sample #3

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
11	3-methyladenine DNA glycosylase I	[U-13C; U-15N]	0.5 ~ 1.0 mM
12	sodium phosphate buffer		10 mM
13	NaCl		100 mM
14	DTT		3 mM
15	NaN3		0.3 mM

9 CBCA(CO)NH

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DMX (750 MHz)

#4 Model Varian UnityPlus (600 MHz)

Sample #1

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Concentration
1	3-methyladenine DNA glycosylase I	0.5 ~ 1.0 mM
2	sodium phosphate buffer	10 mM
3	NaCl	100 mM
4	DTT	3 mM
5	NaN3	0.3 mM

Sample #2

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
6	3-methyladenine DNA glycosylase I	[U-15N]	0.5 ~ 1.0 mM
7	sodium phosphate buffer		10 mM
8	NaCl		100 mM
9	DTT		3 mM
10	NaN3		0.3 mM

Sample #3

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
11	3-methyladenine DNA glycosylase I	[U-13C; U-15N]	0.5 ~ 1.0 mM
12	sodium phosphate buffer		10 mM
13	NaCl		100 mM
14	DTT		3 mM
15	NaN3		0.3 mM

10 HBHA(CO)NH

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DMX (750 MHz)

#4 Model Varian UnityPlus (600 MHz)

Sample #1

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Concentration
1	3-methyladenine DNA glycosylase I	0.5 ~ 1.0 mM
2	sodium phosphate buffer	10 mM
3	NaCl	100 mM
4	DTT	3 mM
5	NaN3	0.3 mM

Sample #2

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
6	3-methyladenine DNA glycosylase I	[U-15N]	0.5 ~ 1.0 mM
7	sodium phosphate buffer		10 mM
8	NaCl		100 mM
9	DTT		3 mM
10	NaN3		0.3 mM

Sample #3

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
11	3-methyladenine DNA glycosylase I	[U-13C; U-15N]	0.5 ~ 1.0 mM
12	sodium phosphate buffer		10 mM
13	NaCl		100 mM
14	DTT		3 mM
15	NaN3		0.3 mM

11 HCCH-TOCSY

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DMX (750 MHz)

#4 Model Varian UnityPlus (600 MHz)

Sample #1

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Concentration
1	3-methyladenine DNA glycosylase I	0.5 ~ 1.0 mM
2	sodium phosphate buffer	10 mM
3	NaCl	100 mM
4	DTT	3 mM
5	NaN3	0.3 mM

Sample #2

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
6	3-methyladenine DNA glycosylase I	[U-15N]	0.5 ~ 1.0 mM
7	sodium phosphate buffer		10 mM
8	NaCl		100 mM
9	DTT		3 mM
10	NaN3		0.3 mM

Sample #3

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
11	3-methyladenine DNA glycosylase I	[U-13C; U-15N]	0.5 ~ 1.0 mM
12	sodium phosphate buffer		10 mM
13	NaCl		100 mM
14	DTT		3 mM
15	NaN3		0.3 mM

12 HCCH-COSY

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DMX (750 MHz)

#4 Model Varian UnityPlus (600 MHz)

Sample #1

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Concentration
1	3-methyladenine DNA glycosylase I	0.5 ~ 1.0 mM
2	sodium phosphate buffer	10 mM
3	NaCl	100 mM
4	DTT	3 mM
5	NaN3	0.3 mM

Sample #2

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
6	3-methyladenine DNA glycosylase I	[U-15N]	0.5 ~ 1.0 mM
7	sodium phosphate buffer		10 mM
8	NaCl		100 mM
9	DTT		3 mM
10	NaN3		0.3 mM

Sample #3

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
11	3-methyladenine DNA glycosylase I	[U-13C; U-15N]	0.5 ~ 1.0 mM
12	sodium phosphate buffer		10 mM
13	NaCl		100 mM
14	DTT		3 mM
15	NaN3		0.3 mM

13 15N-edited TOCSY

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DMX (750 MHz)

#4 Model Varian UnityPlus (600 MHz)

Sample #1

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Concentration
1	3-methyladenine DNA glycosylase I	0.5 ~ 1.0 mM
2	sodium phosphate buffer	10 mM
3	NaCl	100 mM
4	DTT	3 mM
5	NaN3	0.3 mM

Sample #2

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
6	3-methyladenine DNA glycosylase I	[U-15N]	0.5 ~ 1.0 mM
7	sodium phosphate buffer		10 mM
8	NaCl		100 mM
9	DTT		3 mM
10	NaN3		0.3 mM

Sample #3

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
11	3-methyladenine DNA glycosylase I	[U-13C; U-15N]	0.5 ~ 1.0 mM
12	sodium phosphate buffer		10 mM
13	NaCl		100 mM
14	DTT		3 mM
15	NaN3		0.3 mM

14 15N-edited NOESY (t = 125 ms)

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DMX (750 MHz)

#4 Model Varian UnityPlus (600 MHz)

Sample #1

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Concentration
1	3-methyladenine DNA glycosylase I	0.5 ~ 1.0 mM
2	sodium phosphate buffer	10 mM
3	NaCl	100 mM
4	DTT	3 mM
5	NaN3	0.3 mM

Sample #2

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
6	3-methyladenine DNA glycosylase I	[U-15N]	0.5 ~ 1.0 mM
7	sodium phosphate buffer		10 mM
8	NaCl		100 mM
9	DTT		3 mM
10	NaN3		0.3 mM

Sample #3

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
11	3-methyladenine DNA glycosylase I	[U-13C; U-15N]	0.5 ~ 1.0 mM
12	sodium phosphate buffer		10 mM
13	NaCl		100 mM
14	DTT		3 mM
15	NaN3		0.3 mM

15 13C-edited NOESY aliphatic (t = 125 ms)

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DMX (750 MHz)

#4 Model Varian UnityPlus (600 MHz)

Sample #1

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Concentration
1	3-methyladenine DNA glycosylase I	0.5 ~ 1.0 mM
2	sodium phosphate buffer	10 mM
3	NaCl	100 mM
4	DTT	3 mM
5	NaN3	0.3 mM

Sample #2

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
6	3-methyladenine DNA glycosylase I	[U-15N]	0.5 ~ 1.0 mM
7	sodium phosphate buffer		10 mM
8	NaCl		100 mM
9	DTT		3 mM
10	NaN3		0.3 mM

Sample #3

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
11	3-methyladenine DNA glycosylase I	[U-13C; U-15N]	0.5 ~ 1.0 mM
12	sodium phosphate buffer		10 mM
13	NaCl		100 mM
14	DTT		3 mM
15	NaN3		0.3 mM

16 13C-edited NOESY aromatic (t = 125 ms)

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DMX (750 MHz)

#4 Model Varian UnityPlus (600 MHz)

Sample #1

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Concentration
1	3-methyladenine DNA glycosylase I	0.5 ~ 1.0 mM
2	sodium phosphate buffer	10 mM
3	NaCl	100 mM
4	DTT	3 mM
5	NaN3	0.3 mM

Sample #2

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
6	3-methyladenine DNA glycosylase I	[U-15N]	0.5 ~ 1.0 mM
7	sodium phosphate buffer		10 mM
8	NaCl		100 mM
9	DTT		3 mM
10	NaN3		0.3 mM

Sample #3

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
11	3-methyladenine DNA glycosylase I	[U-13C; U-15N]	0.5 ~ 1.0 mM
12	sodium phosphate buffer		10 mM
13	NaCl		100 mM
14	DTT		3 mM
15	NaN3		0.3 mM

17 4D 13C,13C-edited NOESY (t = 100 ms)

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Bruker DMX (750 MHz)

#4 Model Varian UnityPlus (600 MHz)

Sample #1

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Concentration
1	3-methyladenine DNA glycosylase I	0.5 ~ 1.0 mM
2	sodium phosphate buffer	10 mM
3	NaCl	100 mM
4	DTT	3 mM
5	NaN3	0.3 mM

Sample #2

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
6	3-methyladenine DNA glycosylase I	[U-15N]	0.5 ~ 1.0 mM
7	sodium phosphate buffer		10 mM
8	NaCl		100 mM
9	DTT		3 mM
10	NaN3		0.3 mM

Sample #3

Temperature 293 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
11	3-methyladenine DNA glycosylase I	[U-13C; U-15N]	0.5 ~ 1.0 mM
12	sodium phosphate buffer		10 mM
13	NaCl		100 mM
14	DTT		3 mM
15	NaN3		0.3 mM

nullKeywords TAG, 3-Methyladenine DNA glycosylase I, helix-hairpin-helix superfamily, DNA repair

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Found 1 Document (1.786 seconds)

BMRB: 5398

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Chemical shift reference

Chemical shift reference

Related entities 1. 3-methyladenine DNA glycosylase I, : 1 : 2 : 47 entities Detail

Interaction partners 1. 3-methyladenine DNA glycosylase I, : 3 interactors Detail

Experiments performed 17 experiments Detail

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