BMRBj - BMREntryMaster

Found 1 Document (1.783 seconds)

BMRB: 5399

1SXE

Conservation of chemical shift and secondary structure of the PNT/SAM domains from the Ets family of transcription factors

Authors

Mackereth, C.D., Schaerpf, M., Gentile, L.N., McIntosh, L.P.

Assembly

Erg Pointed Domain

Entity

1. Erg Pointed Domain (polymer, Thiol state: not present), 97 monomers, 11380.87 Da Detail

GSHMEEKHMP PPNMTTNERR VIVPADPTLW STDHVRQWLE WAVKEYGLPD VNILLFQNID GKELCKMTKD DFQRLTPSYN ADILLSHLHY LRETPLP

Formula weight

11380.87 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.9 %, Completeness: 91.8 %, Completeness (bb): 94.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	91.8 % (1079 of 1175)	90.1 % (554 of 615)	92.8 % (428 of 461)	98.0 % (97 of 99)
Backbone	94.0 % (530 of 564)	94.1 % (177 of 188)	92.7 % (267 of 288)	97.7 % (86 of 88)
Sidechain	90.6 % (639 of 705)	88.3 % (377 of 427)	94.0 % (251 of 267)	100.0 % (11 of 11)
Aromatic	96.0 % (96 of 100)	96.0 % (48 of 50)	95.7 % (45 of 47)	100.0 % (3 of 3)
Methyl	98.1 % (102 of 104)	100.0 % (52 of 52)	96.2 % (50 of 52)

1. Erg Pointed Domain

GSHMEEKHMP PPNMTTNERR VIVPADPTLW STDHVRQWLE WAVKEYGLPD VNILLFQNID GKELCKMTKD DFQRLTPSYN ADILLSHLHY LRETPLP

Show sample condition

Sample

Temperature 303 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Erg Pointed Domain	[U-99% 13C; U-99% 15N]		1.5 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.07 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.07 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.08 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.4 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 14 models in PDB: 1SXE, Strand ID: A Detail

Release date

2002-11-03

Citation

Letters to the Editor: New NMR assignments chemical shift and secondary structure conservation of the PNT/SAM domains from the Ets family of transcription factors
Mackereth, C.D., Scharpf, M., Gentile, L.N., McIntosh, L.P.
J. Biomol. NMR (2002), 24, 71-72, PubMed , DOI:

Related entities 1. Erg Pointed Domain, : 1 : 3 : 43 entities Detail

Interaction partners 1. Erg Pointed Domain, : 17 interactors Detail

More details for 17 interactors identified by 8 citations

Experiments performed 9 experiments Detail

1 1H-15N HSQC

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian Unity (500 MHz)

Sample #1

Temperature 303 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Erg Pointed Domain	[U-99% 15N]		0.5 ~ 2.5 mM

Sample #2

Temperature 303 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Erg Pointed Domain	[U-99% 13C; U-99% 15N]		1.5 mM

Sample #3

Temperature 303 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Erg Pointed Domain	[U-10% 13C; U-99% 15N]		1.5 mM

2 1H-13C HSQC

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian Unity (500 MHz)

Sample #1

Temperature 303 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Erg Pointed Domain	[U-99% 15N]		0.5 ~ 2.5 mM

Sample #2

Temperature 303 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Erg Pointed Domain	[U-99% 13C; U-99% 15N]		1.5 mM

Sample #3

Temperature 303 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Erg Pointed Domain	[U-10% 13C; U-99% 15N]		1.5 mM

3 HNCACB

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian Unity (500 MHz)

Sample #1

Temperature 303 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Erg Pointed Domain	[U-99% 15N]		0.5 ~ 2.5 mM

Sample #2

Temperature 303 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Erg Pointed Domain	[U-99% 13C; U-99% 15N]		1.5 mM

Sample #3

Temperature 303 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Erg Pointed Domain	[U-10% 13C; U-99% 15N]		1.5 mM

4 HBCBCACONH

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian Unity (500 MHz)

Sample #1

Temperature 303 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Erg Pointed Domain	[U-99% 15N]		0.5 ~ 2.5 mM

Sample #2

Temperature 303 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Erg Pointed Domain	[U-99% 13C; U-99% 15N]		1.5 mM

Sample #3

Temperature 303 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Erg Pointed Domain	[U-10% 13C; U-99% 15N]		1.5 mM

5 HNCO

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian Unity (500 MHz)

Sample #1

Temperature 303 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Erg Pointed Domain	[U-99% 15N]		0.5 ~ 2.5 mM

Sample #2

Temperature 303 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Erg Pointed Domain	[U-99% 13C; U-99% 15N]		1.5 mM

Sample #3

Temperature 303 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Erg Pointed Domain	[U-10% 13C; U-99% 15N]		1.5 mM

6 HACAN

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian Unity (500 MHz)

Sample #1

Temperature 303 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Erg Pointed Domain	[U-99% 15N]		0.5 ~ 2.5 mM

Sample #2

Temperature 303 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Erg Pointed Domain	[U-99% 13C; U-99% 15N]		1.5 mM

Sample #3

Temperature 303 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Erg Pointed Domain	[U-10% 13C; U-99% 15N]		1.5 mM

7 H(CCO)NH-TOCSY

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian Unity (500 MHz)

Sample #1

Temperature 303 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Erg Pointed Domain	[U-99% 15N]		0.5 ~ 2.5 mM

Sample #2

Temperature 303 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Erg Pointed Domain	[U-99% 13C; U-99% 15N]		1.5 mM

Sample #3

Temperature 303 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Erg Pointed Domain	[U-10% 13C; U-99% 15N]		1.5 mM

8 C(CO)NH-TOCSY

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian Unity (500 MHz)

Sample #1

Temperature 303 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Erg Pointed Domain	[U-99% 15N]		0.5 ~ 2.5 mM

Sample #2

Temperature 303 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Erg Pointed Domain	[U-99% 13C; U-99% 15N]		1.5 mM

Sample #3

Temperature 303 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Erg Pointed Domain	[U-10% 13C; U-99% 15N]		1.5 mM

9 HHCH-TOCSY

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian Unity (500 MHz)

Sample #1

Temperature 303 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Erg Pointed Domain	[U-99% 15N]		0.5 ~ 2.5 mM

Sample #2

Temperature 303 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Erg Pointed Domain	[U-99% 13C; U-99% 15N]		1.5 mM

Sample #3

Temperature 303 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Erg Pointed Domain	[U-10% 13C; U-99% 15N]		1.5 mM

nullKeywords PNT/SAM domain, secondary chemical shift, homology

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Found 1 Document (1.783 seconds)

BMRB: 5399

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. Erg Pointed Domain, : 1 : 3 : 43 entities Detail

Interaction partners 1. Erg Pointed Domain, : 17 interactors Detail

Experiments performed 9 experiments Detail

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