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BMRB: 5407

1LM0

Solution structure and characterization of the heme chaperone CcmE

Authors

Arnesano, F., Banci, L., Barker, P.D., Bertini, I., Rosato, A., Su, X.C., Viezzoli, M.S.

Assembly

cytochrome c maturation protein E

Entity

1. cytochrome c maturation protein E (polymer, Thiol state: not present), 161 monomers, 17357.61 Da Detail

MNPRRKKRLT LAVALIGGVA AIASLLLYAL NSNLNLFYTP SEIVNGKTDT GVKPEAGQRI RVGGMVTVGS MVRDPNSLHV QFAVHDSLGG EILVTYDDLL PDLFREGQGI VAQGVLGEDG KLAATEVLAK HDENYMPPEV AEAMGQKHEK LDYSQQKSAT Q

Formula weight

17357.61 Da

Source organism

Shewanella putrefaciens

Exptl. method

NMR

Refine. method

simulated annealing, torsion angle dyanamics, restrained energy minimization

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 77.0 %, Completeness: 68.8 %, Completeness (bb): 74.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	68.8 % (1246 of 1812)	68.3 % (640 of 937)	68.1 % (481 of 706)	74.0 % (125 of 169)
Backbone	74.7 % (711 of 952)	74.3 % (246 of 331)	74.5 % (348 of 467)	76.0 % (117 of 154)
Sidechain	63.9 % (642 of 1005)	65.0 % (394 of 606)	62.5 % (240 of 384)	53.3 % (8 of 15)
Aromatic	24.4 % (21 of 86)	48.8 % (21 of 43)	0.0 % (0 of 43)
Methyl	70.9 % (146 of 206)	69.9 % (72 of 103)	71.8 % (74 of 103)

1. ccme

MNPRRKKRLT LAVALIGGVA AIASLLLYAL NSNLNLFYTP SEIVNGKTDT GVKPEAGQRI RVGGMVTVGS MVRDPNSLHV QFAVHDSLGG EILVTYDDLL PDLFREGQGI VAQGVLGEDG KLAATEVLAK HDENYMPPEV AEAMGQKHEK LDYSQQKSAT Q

Show sample condition

Sample

Pressure 1 atm, Temperature 298 (±1) K, pH 7.0 (±0.05)

#	Name	Isotope labeling	Concentration
1	ccme	[U-13C; U-15N]	2.0 mM
2	phosphate		20 mM
3	H2O		90 %
4	D2O		10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	TMS	methyl protons	internal	direct	1.0
¹³C	TMS	methyl protons	external	indirect	0.2514495
¹⁵N	TMS	methyl protons	external	indirect	0.1013291

Referencing offset: 2.35 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	TMS	methyl protons	internal	direct	1.0
¹³C	TMS	methyl protons	external	indirect	0.2514495
¹⁵N	TMS	methyl protons	external	indirect	0.1013291

Referencing offset: 2.35 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	TMS	methyl protons	internal	direct	1.0
¹³C	TMS	methyl protons	external	indirect	0.2514495
¹⁵N	TMS	methyl protons	external	indirect	0.1013291

Referencing offset: 0.06 ppm, Outliers: 3 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	TMS	methyl protons	internal	direct	1.0
¹³C	TMS	methyl protons	external	indirect	0.2514495
¹⁵N	TMS	methyl protons	external	indirect	0.1013291

Referencing offset: 3.11 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 35 models in PDB: 1LM0, Strand ID: A Detail

Release date

2003-02-19

Citation

Solution structure and characterization of the heme chaperone CcmE
Arnesano, F., Banci, L., Barker, P.D., Bertini, I., Rosato, A., Su, X.C., Viezzoli, M.S.
Biochemistry (2002), 41, 13587-13594, PubMed 12427019 , DOI: , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Swiss-Prot: Q8EK44 released on 2006-05-30
Title CCME_SHEON Entity Cytochrome c-type biogenesis protein CcmE

Related entities 1. cytochrome c maturation protein E, : 1 : 1 : 1 : 107 entities Detail

Experiments performed 10 experiments Detail

1 2D NOESY