BMRB: 5410

Solution structure of novel non-RGD containing short disintegrin by high resolution NMR

Authors

Moreno-Murciano, P., Monleon, D., Calvete, J., Celda, B.

Assembly

obtustatin

Entity

1. obtustatin (polymer, Thiol state: all disulfide bound), 41 monomers, 4401.118 Da Detail

CTTGPCCRQC KLKPAGTTCW KTSLTSHYCT GKSCDCPLYP G

Formula weight

4401.118 Da

Entity Connection

disulfide 4 Detail

---

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS1:SG	1:CYS10:SG
2	disulfide	sing	1:CYS19:SG	1:CYS36:SG
3	disulfide	sing	1:CYS7:SG	1:CYS34:SG
4	disulfide	sing	1:CYS6:SG	1:CYS29:SG

Source organism

Vipera

Exptl. method

NMR

Refine. method

Manual assignment by Wuthrich protocols and automatic NOESY assignment extension by NOAH

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 86.9 %, Completeness (bb): 97.6 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H
All	86.9 % (199 of 229)	86.9 % (199 of 229)
Backbone	97.6 % (80 of 82)	97.6 % (80 of 82)
Sidechain	81.0 % (119 of 147)	81.0 % (119 of 147)
Aromatic	25.0 % (4 of 16)	25.0 % (4 of 16)
Methyl	78.6 % (11 of 14)	78.6 % (11 of 14)

1. obtustatin

CTTGPCCRQC KLKPAGTTCW KTSLTSHYCT GKSCDCPLYP G

Show sample condition

Sample

Temperature 298 (±0.2) K

#	Name	Isotope labeling	Type	Concentration
1	obtustatin			1.1 mM

Hide sample codition

Protein Blocks Logo

Calculated from 22 models in PDB: 1MPZ, Strand ID: A Detail

---

Release date

2002-07-02

Citation

Amino acid sequence and homology modeling of obtustatin, a novel non-RGD-containing short disintegrin isolated from the venom of Vipera lebetina obtusa
Moreno-Murciano, P., Monleon, D., Calvete, J., Celda, B., Marcinkiewicz, C.
Protein Sci. (2003), 12, 366-371, PubMed 12538900 , DOI 10.1110/ps.0230203 , Abstract

Related entities 1. obtustatin, : 1 : 1 : 1 : 210 entities Detail

Experiments performed 2 experiments Detail