BMRB: 5459

High precision NMR structure of RING-H2 finger domain of EL5, a rice gene in response to N-acetylchitooligosaccahride elicitor

Authors

Yamazaki, T.

Assembly

EL5 RING-H2 finger domain

Entity

1. EL5 RING-H2 finger domain (polymer, Thiol state: not reported), 55 monomers, 5979.797 Da Detail

AMDDGVECAV CLAELEDGEE ARFLPRCGHG FHAECVDMWL GSHSTCPLCR LTVVV

Formula weight

5979.797 Da

Source organism

Oryza sativa

Exptl. method

NMR

Refine. method

distance geometry simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 95.2 %, Completeness (bb): 97.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	95.2 % (554 of 582)	94.2 % (278 of 295)	96.1 % (224 of 233)	96.3 % (52 of 54)
Backbone	97.9 % (319 of 326)	96.5 % (109 of 113)	99.4 % (159 of 160)	96.2 % (51 of 53)
Sidechain	92.8 % (284 of 306)	92.9 % (169 of 182)	92.7 % (114 of 123)	100.0 % (1 of 1)
Aromatic	72.7 % (32 of 44)	72.7 % (16 of 22)	71.4 % (15 of 21)	100.0 % (1 of 1)
Methyl	98.4 % (61 of 62)	96.8 % (30 of 31)	100.0 % (31 of 31)

1. RING-H2 finger domain

AMDDGVECAV CLAELEDGEE ARFLPRCGHG FHAECVDMWL GSHSTCPLCR LTVVV

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Sample

Temperature 308 (±0.2) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	RING-H2 finger domain	[U-13C; U-15N]		0.0 ~ 0.0 mM

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LACS Plot; CA

Referencing offset: -0.37 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.37 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.13 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.15 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 15 models in PDB: 1IYM, Strand ID: A Detail

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Release date

2003-06-11

Citation

High precision NMR structure and function of the RING-H2 finger domain of EL5, a rice protein whose expression is increased upon exposure to pathogen-derived oligosaccharides
Katoh, S., Hong, C., Tsunoda, Y., Murata, K., Takai, R., Minami, E., Yamazaki, T., Katoh, E.
J. Biol. Chem. (2003), 278, 15341-15348, PubMed 12588869 , DOI 10.1074/jbc.M210531200 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. EL5 RING-H2 finger domain, : 1 : 2 : 280 entities Detail

Experiments performed 1 experiments Detail