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Solution Structure of a CCHC Zinc Finger from MOZ
Authors
Kwan, A.H.Y., Gell, D.A., Liew, C.K., Mackay, J.P.
Assembly
Monocytic leukemia zinc finger protein
Entity
1. Zinc finger protein (polymer, Thiol state: all other bound), 31 monomers, 3893.774 Da Detail

RLPKLYLCEF CLKYMKSRTI LQQHMKKCGW F


2. ZN (non-polymer), 65.409 Da
Total weight
3959.1829 Da
Max. entity weight
3893.774 Da
Entity Connection
metal coordination 4 Detail

IDTypeValue orderAtom ID 1Atom ID 2
1metal coordinationsing1:CYS8:SG2:ZN1:ZN
2metal coordinationsing1:CYS11:SG2:ZN1:ZN
3metal coordinationsing1:HIS24:NE22:ZN1:ZN
4metal coordinationsing1:CYS28:SG2:ZN1:ZN

Source organism
Homo sapiens
Exptl. method
NMR
Refine. method
simulated annealing molecular dynamics torsion angle dynamics
Data set
assigned_chemical_shifts, coupling_constants
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 85.0 %, Completeness (bb): 100.0 % Detail

Polymer type: polypeptide(L)

Total1H
All85.0 % (192 of 226)85.0 % (192 of 226)
Backbone100.0 % (62 of 62)100.0 % (62 of 62)
Sidechain79.3 % (130 of 164)79.3 % (130 of 164)
Aromatic61.5 % (16 of 26)61.5 % (16 of 26)
Methyl92.3 % (12 of 13)92.3 % (12 of 13)

1. Zinc finger protein

RLPKLYLCEF CLKYMKSRTI LQQHMKKCGW F

Sample

Pressure 1 atm, Temperature 298 (±0.2) K, pH 5.6 (±0.1)


#NameIsotope labelingTypeConcentration
1Zinc finger protein0.5 mM
2ZINC (II) ION0.7 mM
3TCEP1 mM
4H2O95 %
5D2O5 %

Protein Blocks Logo
Calculated from 20 models in PDB: 1M36, Strand ID: A Detail


Coupling constant
5 J values in 1 lists
Field strength (1H) 600 MHz, Pressure 1 atm, Temperature 298 (±0.2) K, pH 5.6 (±0.1) Detail
Release date
2004-09-13
Citation
Solution Structure of a CCHC Zinc Finger from MOZ
Kwan, A.H.Y., Gell, D.A., Liew, C.K., Mackay, J.P.
Related entities 1. Zinc finger protein, : 1 : 8 : 132 entities Detail
Interaction partners 1. Zinc finger protein, : 17 interactors Detail
Experiments performed 3 experiments Detail
nullKeywords zinc finger, acetyl transferase