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Found 1 Document (1.661 seconds)

BMRB: 5467

1RI9

1H,15N and 13C resonance assignments of the SH3-like domain of the human ADAP protein

Authors

Gupta, P., Langdon, G.M., Kofler, M.M., Schmidt, S., Heuer, K.J., Thiemke, K., Freund, C.

Assembly

ADAP

Entity

1. ADAP (polymer, Thiol state: all free), 102 monomers, 11875.18 Da Detail

MGSSHHHHHH SSGKKLKKQE KEEKDFRKKF KYDGEIRVLY STKVTTSITS KKWGTRDLQV KPGESLEVIQ TTDDTKVLCR NEEGKYGYVL RSYLADNDGE IY

Formula weight

11875.18 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 87.3 %, Completeness: 76.8 %, Completeness (bb): 81.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	76.8 % (941 of 1225)	77.7 % (501 of 645)	74.6 % (353 of 473)	81.3 % (87 of 107)
Backbone	81.1 % (495 of 610)	82.5 % (174 of 211)	80.2 % (239 of 298)	81.2 % (82 of 101)
Sidechain	73.9 % (524 of 709)	75.3 % (327 of 434)	71.4 % (192 of 269)	83.3 % (5 of 6)
Aromatic	64.4 % (67 of 104)	80.8 % (42 of 52)	47.1 % (24 of 51)	100.0 % (1 of 1)
Methyl	95.3 % (82 of 86)	97.7 % (42 of 43)	93.0 % (40 of 43)

1. SH3 like domain of ADAP-binding protein

MGSSHHHHHH SSGKKLKKQE KEEKDFRKKF KYDGEIRVLY STKVTTSITS KKWGTRDLQV KPGESLEVIQ TTDDTKVLCR NEEGKYGYVL RSYLADNDGE IY

Show sample condition

Sample

Temperature 300 (±0.2) K, pH 6.3 (±0.02)

#	Name	Isotope labeling	Type	Concentration
1	SH3 like domain of ADAP-binding protein	[U-95% 13C; U-90% 15N]		0.5 ~ 1.8 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.28 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.28 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.01 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.23 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1RI9, Strand ID: A Detail

Release date

2004-10-12

Citation

Structure of a helically extended SH3 domain of the T cell adapter protein ADAP
Heuer, K.J., Kofler, M.M., Langdon, G.M., Thiemke, K., Freund, C.
Structure (2004), 12, 603-610, PubMed 15062083 , DOI 10.1016/j.str.2004.02.021 , Abstract

Related entities 1. ADAP, : 1 : 2 : 9 entities Detail

Interaction partners 1. ADAP, : 11 interactors Detail

More details for 11 interactors identified by 8 citations

Experiments performed 11 experiments Detail

1 1H-15N HSQC