BMRB: 5470

Inhibitory Specificity Change of Ovomucoid Third Domain of Silver Pheasant upon Introduction of an Engineered Cys14-Cys39 Bond

Authors

Hemmi, H., Kumazaki, T., Yamazaki, T., Kojima, S., Yoshida, T., Kyogoku, Y., Katsu, M., Shinohara, F., Yokosawa, H., Miura, K., Kobayashi, Y.

Assembly

p14c/n39c monomer

Entity

1. p14c/n39c monomer (polymer, Thiol state: all disulfide bound), 54 monomers, 5849.630 Da Detail

AVSVDCSEYP KCACTMEYRP LCGSDNKTYG NKCNFCCAVV ESNGTLTLSH FGKC

Formula weight

5849.63 Da

Entity Connection

disulfide 4 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS6:SG	1:CYS36:SG
2	disulfide	sing	1:CYS12:SG	1:CYS37:SG
3	disulfide	sing	1:CYS14:SG	1:CYS33:SG
4	disulfide	sing	1:CYS22:SG	1:CYS54:SG

Source organism

Lophura nycthemera

Exptl. method

NMR

Refine. method

distance geometry, simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 95.6 %, Completeness (bb): 95.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	95.6 % (285 of 298)	95.6 % (285 of 298)
Backbone	95.5 % (105 of 110)	95.5 % (105 of 110)
Sidechain	95.7 % (180 of 188)	95.7 % (180 of 188)
Aromatic	91.7 % (22 of 24)	91.7 % (22 of 24)
Methyl	100.0 % (21 of 21)	100.0 % (21 of 21)

1. ovomucoid third domain from silver pheasant

AVSVDCSEYP KCACTMEYRP LCGSDNKTYG NKCNFCCAVV ESNGTLTLSH FGKC

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Sample

Temperature 298 (±1) K, pH 4.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	ovomucoid third domain from silver pheasant			4.0 mM

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Protein Blocks Logo

Calculated from 15 models in PDB: 1IY6, Strand ID: A Detail

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Release date

2002-07-22

Citation

Inhibitory specificity change of the ovomucoid third domain of the silver pheasant upon introduction of an engineered Cys14-Cys39 bond
Hemmi, H., Kumazaki, T., Yamazaki, T., Kojima, S., Yoshida, T., Kyogoku, Y., Katsu, M., Shinohara, F., Yokosawa, H., Miura, K., Kobayashi, Y.
Biochemistry (2003), 42, 2524-2534, PubMed 12614146 , DOI 10.1021/bi026727c , Abstract

Related entities 1. p14c/n39c monomer, : 1 : 34 : 226 entities Detail

Interaction partners 1. p14c/n39c monomer, : 3 interactors Detail

Experiments performed 3 experiments Detail