BMRB: 5481

Backbone and side chain 1H, 13C, and 15N chemical shift assignments for S. cerevisae Hub1

Authors

Ramelot, T.A., Kennedy, M.A.

Assembly

Hub1

Entity

1. Hub1 (polymer, Thiol state: all free), 93 monomers, 10434.77 Da Detail

MGSSHHHHHH SSGLVPRGSH MIEVVVNDRL GKKVRVKCLA EDSVGDFKKV LSLQIGTQPN KIVLQKGGSV LKDHISLEDY EVHDQTNLEL YYL

Formula weight

10434.77 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

NMR

Refine. method

distance geometry and simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 82.8 %, Completeness: 78.2 %, Completeness (bb): 80.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	78.2 % (845 of 1081)	77.0 % (432 of 561)	78.9 % (333 of 422)	81.6 % (80 of 98)
Backbone	80.0 % (443 of 554)	78.1 % (150 of 192)	80.4 % (218 of 271)	82.4 % (75 of 91)
Sidechain	77.3 % (473 of 612)	76.4 % (282 of 369)	78.8 % (186 of 236)	71.4 % (5 of 7)
Aromatic	28.6 % (20 of 70)	28.6 % (10 of 35)	28.6 % (10 of 35)
Methyl	92.7 % (102 of 110)	92.7 % (51 of 55)	92.7 % (51 of 55)

1. Hub1

MGSSHHHHHH SSGLVPRGSH MIEVVVNDRL GKKVRVKCLA EDSVGDFKKV LSLQIGTQPN KIVLQKGGSV LKDHISLEDY EVHDQTNLEL YYL

Show sample condition

Sample #1

Temperature 298 (±1) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Hub1	[U-15N; U-13C]		1.0 ~ 3.0 mM
2	acetate buffer			10 mM
3	NaCl			300 mM

Sample #2

Temperature 298 (±1) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	Hub1	[U-15N]		1.0 ~ 3.0 mM
5	acetate buffer			10 mM
6	NaCl			300 mM

Sample #3

Temperature 298 (±1) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
7	Hub1	[U-10% 13C; U-15N]		1.0 ~ 3.0 mM
8	acetate buffer			10 mM
9	NaCl			300 mM

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LACS Plot; CA

Referencing offset: -0.02 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CB

Referencing offset: -0.02 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; HA

Referencing offset: -0.1 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CO

Referencing offset: 0.29 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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Protein Blocks Logo

Calculated from 20 models in PDB: 1M94, Strand ID: A Detail

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Release date

2003-07-29

Citation

Solution structure of the yeast ubiquitin-like modifier protein Hub1
Ramelot, T.A., Cort, R.J., Yee, A.A., Semesi, A., Edwards, A.M., Arrowsmith, C.H., Kennedy, M.A.
J. Struct. Funct. Genomics (2003), 4, 25-30, PubMed 12943364 , Abstract

Related entities 1. Hub1, : 1 : 2 : 352 entities Detail

Experiments performed 2 experiments Detail